Philippine ethnobotanicals inhibit virulence factors in Staphylococcus aureus

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Short Communications 01/11/2018
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Philippine ethnobotanicals inhibit virulence factors in Staphylococcus aureus

Gene Beniece B. Limos, Wilson R. Jacinto, Khristina G. Judan Cruz
Int. J. Biosci.13( 5), 178-187, November 2018.
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A new perspective in the application of natural compounds as therapeutic agents are presented by quorum sensing inhibitors to combat pathogenic diseases without developing antimicrobial resistance.  The study evaluated the quorum sensing inhibition (QSI) activities of ethanolic extracts of selected Philippine ethnobotanicals against Staphylococcus aureus PNCM 1582. Ethnobotanicals tested were Bidens pilosa, Cestrum nocturnum, Sarcandra glabra, Oreocnide trinervis, Pittosporum pentandrum, Lipang-daga (no known scientific name), Derris elliptica, Alstonia scholaris, Ageratina adenophora and Ayapana triplinervis Extracts were subjected to assays on the phenotypic expression of virulence factors of S. aureus: DNase and α-hemolysin. C. nocturnum, O. trinervis and A. triplinervis showed inhibition of the phenotypic expression of DNase in S. aureus indicating the presence of QSI.C. nocturnum, S. glabra, O. trinervis, Lipang-daga, D. elliptica, A. scholaris, A. adenophora  and A. triplinervis ethanolic plant extracts caused QSI resulting in the absence of α-hemolysin production in the plate culture.  Findings of both virulence assays confirm the QSI activities of the ethnobotanicals against the virulence factors in S. aureus.  Thus, these ethnobotanicals display potential for the development of anti-quorum sensing drugs to combat bacterial infections without developing resistance.


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