Phosphate removing using bacteria isolated from activated sludge for wastewater remediation

Paper Details

Research Paper 01/05/2015
Views (258) Download (9)

Phosphate removing using bacteria isolated from activated sludge for wastewater remediation

Oumaima Naili, Messaoud Benounis, Leyla Benammar
J. Bio. Env. Sci.6( 5), 34-41, May 2015.
Certificate: JBES 2015 [Generate Certificate]


The objective of the current study is to isolate phosphate removing microbes from activated sludge for wastewater remediation in the area of Khenchela (Eastern Algeria) . Phosphate rate was determined using colorimetric method and batch tests were developed to evaluate the biomass composition of the sludge. Four efficient pure strains isolated from activated sludge samples and identified as Acinetobacter junii, Pseudomonas aeruginosa, Moraxella lacunata, Alcaligenes denitrificans were tested for their capacities of phosphate reduction. The best rate of phosphate removal by pure cultures is observed with Acinetobacterjunii, 76%, followed by Alcaligenes denitrificans, 70.42%, Pseudomonas aeruginosa, 61.78%, and Moraxella lacunata, 50.6%. Our results show that applying of mixed bacterial culture containing mostly isolated strains for bioremediation purpose can be used successfully for the elimination of phosphate from activated sludge of wastewater treatment plants.


Anderson DM, Glibert PM, Burkholder JM. 2002. Harmful algal blooms and eutrophication: nutrient sources, composition, and consequences. Estuaries 25, 704–726.

Bao L, Li D, Li X. 2007. Phosphorus accumulation by bacteria isolated from a continuous-flow two-sludge system. J. Environ. Sci 19, 391–395.

itton G. 2005. Wastewater Microbiology. 3ed Edition. John Wiley & Sons.

Cohen GN. 2011. Microbial Biochemistry. 2nd Edition. Springer.

Florentz M, Hartemann P. 1984. Screening for phosphate accumulating bacteria isolated from activated sludge. Env. Tech. Letters 5, 457-463.

Greaves J, Hobbs P, Chadwick D. 1999. Prospect for the recovery of phosphorus from animal manures, A review. Environ. Technol 20, 697-708.

Krishnaswamy U, Muthusamy M, Perumal-samy L. 2009. Studies on the Efficiency of the Removal of Phosphate Using Bacterial Consortium for the Biotreatment of Phosphate Wastewater. European Journal of Applied Sciences 1, 06-15.

Kuba T, Van Loosdrecht MCM, Heijnen JJ. 1997. Biological dephosphatation by activated sludge under denitrifying conditions pH influence and occurrence of denitrifying. dephosphatation in a full-scale waste water treatment plant, Water Science and Technology 36 ,75-82.

Lee H, Yun Z. 2014. Comparison of biochemical characteristics between PAO and DPAO sludges. Journal of Environmental Sciences 26, 1340-1347.

Merzouki M, Delgenès JP, Bernet N. 1999. Polyphosphate Accumulating and Denitrifying Bacteria Isolated from Anaerobic-Anoxic and Anaerobic-Aerobic  Sequencing  Batch  Reactors. Cur. Microbiol 38, 9-17.

Mielcarek A, Rodziewicz J, Janczukowicz W. 2015. The feasibility of citric acid asexternal carbon source for biological phosphorus removal in a sequencing batch biofilm reactor (SBBR). Biochemical Engineering Journal 93, 102-107.

Momba MNB, Cloete TE. 1996. The relationship of biomass to phosphate uptake by Acinetobacterjunii activated sludge mixed liquor. Water Res 30(2), 364–370.

Morohoshi T, Yamashita T, Kato J. 2003. A method for screening polyphosphate accumulating mutants which remove phosphate efficiently from synthetic wastewater. J. Biosci. Bioeng 95(6), 637-40.

Mubyana-John T, Letsamao MC. 2007. Polyphosphate bacteria associated with the glen valley wastewater treatment plant; Gaborone. 8th Water NET/WARFSA/GWP-SA Symposium.…/Mubyana-John.pdf.

Pauli AS, Kaitala S. 1997. Phosphate uptake kinetics by Acinetobacter isolates. Biotechnol. Bioeng 53, 304-309.

Saito T, Brdjanovic D, van Loosdrecht MCM. 2004. Effect of nitrite on phosphate uptake by phosphate accumulating organisms. Water Research 38, 3760-3768.

Serafim LS, Lemos OC, Levantesi C. 2002. Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms. J. Microbiol. Meth 51, 1-18.

Sarioglu M. 2005. Biological phosphorus removal in a sequencing batch reactor by using pure cultures. Proces. Biochem 40, 1599–1603.

Sidat M, Bux F, Kasan H. 1999. Polyphosphate accumulation by bacteria isolated from activated sludge Water SA 25,175-179.

Wrigley  TJ,   Webb   KM,   Venkitachalm    HA. 1992. Laboratory study of struvite precipitation after digestion of piggery wastes. Bioresour. technol 41, 117- 121.

Zafiri C, Kornaros M, Lyberatos G. 1999. Kinetic modelling of biological phosphorus removal with a pure culture of Acinetobacter sp. under aerobic, anaerobic and transient operating conditions. Water Research 33(12), 2769-2788.