Paper Details

Phylogenetic relationship and in silico expression profile of PELPK1 of Arabidopsis thaliana (L.) Heynh

Abdur Rashid, Michael Deyholos

DOI: https://dx.doi.org/10.12692/ijb/6.2.93-99

Int. J. Biosci. 6(2), 93-99. January, 2015. (PDF)

Abstract:

Based on bioinformatics and computational analyses, the Arabidopsis thaliana (L.) (Heynh.) gene, At5g09530 was earlier annotated as PELPK1 and its putative paralog, At5g09520 as PELPK2. In the present report, further in silico analyses were carried out to determine the phylogenetic relationship of PELPK1 with the proteomes of A. thaliana (L.) (Heynh.) and other species of angiosperms; and the expression pattern and inducibility of PELPK1 in Arabidopsis plants. The data suggest that PELPK1 and PELPK2 are closely related and form a subgroup of hydroxyproline rich glycoprotein (HRGP)-family of proteins; PELPK and similar motifs are conserved to large evolutionary distances involving both monocot and dicot; PELPK1 is highly responsive to certain biotic factors and elicitors, moderately responsive to abiotic factors, unresponsive to common growth hormones, and is negatively responsive to natural phytosterols; and that it is predominantly expressed during early and late stages of Arabidopsis growth. The above in silico data correspond partially with our experimental observations reported earlier.