Physico-chemical and microbiological characterizations of an herbal toothpaste formulated with extracts of some selected medicinal plant harvested in Benin Republic
Paper Details
Physico-chemical and microbiological characterizations of an herbal toothpaste formulated with extracts of some selected medicinal plant harvested in Benin Republic
Abstract
Beninese flora is endowed with effective medicinal plants used locally for hygiene and oral care but very little valued. This work therefore aims to promote these medicinal plants through the formulation of an herbal toothpaste. For this purpose, the chemical composition and anti-radical activity of extracts of four plants (Syzygium aromaticum, Zingiber officinale, Vernonia amygdalina, Thevetia peruviana) were determined. Then an antimicrobial toothpaste was formulated by incorporating bioactive extracts from the plants studied. The physico-chemical and antimicrobial properties of the toothpaste formulated were determined. The results reveal the presence of a large number of secondary metabolites and a significant quantity of phenolic compounds in the four plants studied. Syzygium aromaticum and Thevetia peruviana presented the highest contents of total phenolic compounds, respectively 1258.85 and 616.12 µg EGA/g. Plant extracts show strong anti-radical activity with IC50 ranging from 0.1 to 45 µg/µL. Only the essential oils studied showed antioxidant activity close to that of the reference compounds, i.e. 0.029 µg/µL and 0.022 µg/µL respectively for quercetin and gallic acid. The formulated toothpaste has good physicochemical characteristics and acceptable hygienic quality. The antimicrobial activity shown by the toothpaste is interesting with a minimum inhibitory concentration of the toothpaste being 0.100g/mL on most strains tested. Subject to the toxicity results, the formulated product can be recommended for good oral hygiene.
Agbangnan PD, Tachon C, Bonin H, Chrostowka A, Fouquet E, Sohounhloue, DC 2012. Phytochemical study of a dye plant of Benin traditional pharmacopoeia: The red sorghum (sorghum caudatum) of Benin. Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry 13 (2), 121.
Agbankpe A, Bankolé S, Assogba M, Dougnon V, Yèhouénou B, Joachim Djimon G, Baba-Moussa L. 2015. Phytochemical Screening and Cytotoxic Analysis of Three Local Vegetables Used in the Treatment of Bacterial Diarrhea in Southern Benin (West Africa): A Comparative Study 9, 1 – 13. https://doi.org/10.9734/BBJ/2015/19123
Ahouanse, MM. 2015. Ethnobotanical study of plants for oral and dental uses in the commune of Kétou in Benin [Technical Report]. EPAC/UAC/CAP. http://biblionumeric.epac-uac.org:8080/jspui/handle/123456789/176
Ainsworth EA and Gillespie KM. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature protocols 2, 875-877.
Alara OR, Abdurahman NH, Olalere OA. 2018. Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool. Journal of Food Measurement and Characterization 12 (2), 1107 – 1122. https://doi.org/10.1007/s11694-018-9726-3
Alhashimi SKM, Rashid KI, Saleh GS, Abdulhadi AM, Taher TA. 2013. The antimicrobial activity of leaves and callus extracts of Thevetia peruviana In vitro. Journal of Biotechnology Research Center 7 (3), Art. 3. https://doi.org/10.24126/jobrc.2013.7.3.285.
Anyiam IV, Ariyo AB. 2021. Antibacterial Activity of Different Toothpastes and Chewing Sticks on Selected Bacteria Isolated from the Oral Cavity. African Journal of Environment and Natural Science Research 4(2), 27-38. DOI: 10.52589/AJENSR_S8TKVJNZ.
Barbelet S. 2015. The clove tree: History, description and uses of the plant and its essential oil (p. not provided) [Other, University of Lorraine]. https://hal.univ-lorraine.fr/hal-01732523
Bourai A, Azzouk A. 2018. Phytochemical study and antioxidant activity of Zingiber officinale [Thesis, University of Bouira]. http://dspace.univ-bouira.dz:8080/jspui/handle/123456789/3017
Brandy ML, Robert P. 2006. Toothpastes. The MIDIFABS 5, p87-96.
Dehina SR. 2021. Determination of phenolic compounds by HPLC-MS/MS and evaluation of the antioxidant activity of rhizome of “Zingiber Officinale Roscoe”-Article analysis.
Deshmukh P, Telrandhe R, Gunde M. 2017. Formulation and Evaluation of Herbal Toothpaste: Compared With Marketed Preparation. International Journal of Pharmaceutics and Drug Analysis 406 – 410.
Diatta BD. 2021. Oral tattoos in the cosmetopeia of the Peulh of Ferlo (Senegal): Practices, norms, meanings and risks. Body 19 (1) 393 – 412. https://doi.org/10.3917/corp1.019.0393
Djossou D, Kpozehouen A, Nancy J, Houinato D, Pilipili C. 2014. Fluoride and oral health in schools in the commune of Dassa in Benin. French-speaking Journal of Pediatric Odontology 9, 182.
Gbenou BOR. 2014. Ethnobotanical study of Vitex doniana (Sweet) (Verbenaceae) in the commune of Bonou in Benin. [Technical Report]. EPAC/UAC.http://biblionumeric.epac-uac.org:8080/jspui/handle/123456789/61
Girard G. 2010. The properties of essential oils in oral care from yesterday to today. Development of a preclinical model of mouth ulcer type lesions to test the therapeutic effects of essential oils. (p. not specified) [Other, UHP – Henri Poincaré University].https://hal.univ-lorraine.fr/hal-01732627
Guimard G. 2002. Dental fluorosis: Current data and evaluation [PhD Thesis]. UHP-Henri Poincaré University.
Habtamu A, Melaku Y. 2018. Antibacterial and Antioxidant Compounds from the Flower Extracts of Vernonia amygdalina. Advances in Pharmacological Sciences, 2018, e4083736. https://doi.org/10.1155/2018/4083736
Imam UA, Zahrau A, Obeagu EI. 2024. The Antibacterial Effect of some Selected Chewing
Kere M. 2017. Effect of storage time and temperature on the nutritional and microbiological quality of raw milk collected in Burkina Faso. International Journal of Innovation and Scientific Research 29, 23 – 30.
Kokutse EKM. 2016. Evaluation of the phytochemical and antimicrobial properties of five spices and aromatic herbs commonly used in Benin. https://biblionumeric.epac-uac.org:9443/jspui/handle/123456789/1152
Kolenbrander PE, London J. 1993. Adhere today, here tomorrow: oral bacterial
adherence. Bacteria 175, 3247-3252.
Koudoro YA, Agbangnan Dossa CP, Yèhouénou BB, Tchobo FP, Alitonou GA, Avlessi F, Koudoro YA, Awadji JM, Botezatu DA, Olayé T, Agbangnan Dossa CP, Alitonou GA, Avlessi F, Dinica RM and Sohounhloue CKD. 2021. Phytochemical analysis, antioxidant and anti-inflammatory activities of Chassalia kolly leaves extract, a plant used in Benin to treat skin illness ; GSC Biological and Pharmaceutical Sciences 15(03), 063–072.
Koudoro YA, Bogninou GSR, Bossou A FAD, Agbangnan Dossa CP, Olayé T, Bothon FTD, Alitonou GA, Avlessi F and Sohounhloue D. 2018. Secondary metabolites and biological activities of extracts from the trunk bark of Khaya senegalensis, a planta veterinary use harvested in Benin. International Journal of Innovation and Applied Studies 23 (4), 441-450.
Koudoro YA, Tamfu AN, Selcuk K, Ozgur C, Agbangnan Dossa CP, Avlessi F, Sohounhloue CKD, Mehmet ED, Rodica MD. 2022. Phenolic profiles, antioxidant, antiquorum sensing, antibiofilm and enzyme inhibitory activities of selected Acacia species collected from Benin. LWT 171, 114162.
Kpatinvoh B, Adjou ES, Dahouenon-Ahoussi E, Konfo TC, Atrevi B, Soumanou MM, Sohounhloue DC. 2017. Effectiveness of essential oils of three aromatic plants against spoilage mycoflora of cowpea (Vigna unguiculata L., Walp) collected in sales stores in South Benin. Journal of Applied Biosciences 109, 10680-10687.
Lachance M. 2015. The future of the chemical industry. Vector Environment 48(5), 32.
Lubis. 2022. Phytochemical Profile and Pharmacological Activity of Vernonia amygdalina Delile Stem Bark Extracts Using Different Solvent Extraction. Open Access Macedonian Journal of Medical Sciences.https://oamjms.eu/index.php/mjms/article/view/8921
Madagascar Standards Bureau. 2018. Oral medicine—toothpastes—requirements, testing methods and marking.
Makkar HPS. 2000. Quantification of tannins in tree foliage. A laboratory manual for the FAO/IAEA co-ordinated research project on ‘Use of Nuclear and Related Techniques to Develop Simple Tannin Assays for Predicting and Improving the Safety and Efficiency of Feeding Ruminants on Tanniniferous Tree foliage. FAO/IAEA working document IAEA, VIENA (Austria), 14p.
Marrelli M, Menichini F, Conforti F. 2015. A comparative study of Zingiber officinale Roscoe pulp and peel: phytochemical composition and evaluation of antitumor activity. Natural Product Research 29 (21), 2045-2049.
Mathuravalli K, Lakshmi RE. 2012. Analysis of Phytochemical Components and Anti-microbial Activity of the Toxic Plant—Thevetia peruviana. Indian Journal of Innovations and Developments, 1(2), Art. 2. http://ischolar.info/index.php/ijid/article/view/31600
Nesrine A. 2019. Study of the antioxidant and antibacterial activity of methanolic extracts of Curcuma longa L. and Zingiber officinale (Rosc.) marketed in the M’sila region. [Thesis, Mohamed BOUDIAF University of M’Sila]. http://localhost:8080/xmlui/handle/123456789/15334
Nurdjannah N, Bermawie N. 2012. Cloves. In KV Peter (Ed.), Handbook of Herbs and Spices (Second Edition) (pp. 197 – 215). Woodhead Publishing. https://doi.org/10.1533/9780857095671.197
Olayé T, Tchobo FP, Chabi N, Koudokpon H, Amoussa AMO, Lagnika L, Alitonou AG, Avlessi F, Sohounhloué D. 2020. Bioactive compounds and antimicrobial potential of the roots extract of Anogeissus leiocarpa, a chewing stick used for oral care in Benin Republic. Journal of Pharmacognosy and Phytotherapy 12(4), 71-80.
Olayé T. Tchobo FP and Chabi, NW. 2018. Phytochemical potential, antiradical and antimicrobial activity of crude extracts of Caesalpinia benthamiana roots used for oral hygiene in Benin republic. Journal of Pharmacognosy and Phytochemistry 7(5), 1939-1944.
Osabor V, Bassey F, Umoh U. 2015. Phytochemical Screening and Quantitative Evaluation of Nutritional Values of Zingiber officinale (Ginger). American Chemical Science Journal 8, 1 – 6. https://doi.org/10.9734/ACSJ/2015/16915
Petersen PE. 2003. The World Oral Health Report 2003: Continuing to improve oral health in the 21st century-the WHO Oral Health Program approach. World Health Organization.
Pundir RK, Jain P, Sharma C. 2010. Antimicrobial Activity of Ethanolic Extracts of Syzygium aromaticum and Allium sativum Against Food Associated Bacteria and Fungi . Ethnobotanical Leaflets (3), 11.
Qing F, Elumalai M, Akowuah G. 2014. Antimicrobial and Antioxidant Studies of Vernonia Amygdalina. Journal of Applied Pharmacy 6. https://doi.org/10.21065/19204159.6.4.332
Quenum LMG, Azokpota P, Dabade DS, Padonou W, Honfo F, Tchekessi, C. 2020. Microbiological and physicochemical characterization of akassa packaged in different leaf packaging during storage at 30°C. (Memory) http://biblionumeric.epac-uac.org:8080/jspui/handle/123456789/2832
Rajhans S, Mankad AU, Pandya HA. 2019. Screening Thevetia peruviana (Pers.) K. Schum. for its Bioactive Phytochemicals. International Journal of Research and Analytical Reviews 6, 932-35.
Ramzi A, Kamouche AMI. 2021. Evaluation of the anti-radical power of extracts of some spices (Cuminum cyminum, Curcuma longa and Syzygium aromaticum). http://dspace.univ-guelma.dz/jspui/handle/123456789/12035
Regional Committee for Africa. 2016. Regional strategy for oral health 2016-2025: Combating oral diseases as part of the fight against non-communicable diseases: Report from the Secretariat. https://apps.who.int/iris/handle/10665/250988
Rehab M. 2020. Plants used for oral diseases in the Sétif region (Algeria): Ethnobotanical aspects [Thesis, Mohamed Boudiaf University – M’SILA]. http://localhost:8080/xmlui/handle/123456789/20858
Sanou A, Tapsoba F, Zongo C, Savadogo A, Traore Y. 2017. Study of the nutritional and microbiological quality of infant flours from four production units: CMA Saint Camille de Nanoro, CSPS Saint Louis de Temnaoré, CM Saint Camille d’ Ouagadougou and Koudougou CHR. Nature & Technology 17.
Sekar M, Noor Jasmin SA. 2016. Formulation, evaluation and antibacterial properties of novel polyherbal toothpaste for oral care. International Journal of Pharmaceutical and Clinical Research 8(8), 1155-1158.
Selles SMA, Kouidri M, Belhamiti BT, Ait Amrane A. 2020. Chemical composition, in-vitro antibacterial and antioxidant activities of Syzygium aromaticum essential oil. Journal of Food Measurement and Characterization 14 (4), 2352 – 2358. https://doi.org/10.1007/s11694-020-00482-5
Sharma S, Agarwal SS, Prakash J, Pandey M, Singh A. 2014. Formulation Development and Quality Evaluation of Polyherbal Toothpaste” Oral S”. International Journal of Pharmaceutical Research & Allied Sciences 3(2).
Sohounhloué DCK. 2014. Phytochemistry, antimicrobial and antiradical activities evaluation of essential oils, ethanolic and hydroethanolic extracts of the leaves of Eucalyptus citriodora hook from benin. St. Cerc. St. CICBIA 15 (1), 059-073.
Srivastava N, Chauhan AS, Sharma B. 2012. Isolation and Characterization of Some Phytochemicals from Indian Traditional Plants. Biotechnology Research International, 1 – 8. https://doi.org/10.1155/2012/549850
Subramanian S, Appukuttan D, Tadepalli A, Gnana PPS, Victor DJ. 2017. The role of abrasives in toothpastes. Journal of Pharmaceutical Sciences and Research 9(2), 221.
Taroq A, Bakour M, El Atki Y, El Kamari F, Aouam I. Zahra F. 2021. Comparative Study of The Effects of Laurus nobilis and Syzygium aromaticum Aqueous Extracts on Urine Volume and Renal Function In Rats. International Journal of Pharmaceutical Sciences and Research 12 (2), 776-782.
Wang X, Shen Y, Thakur K, Han J, Zhang JG, Hu F, Wei ZJ. 2020. Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus. Molecules 25(17), Art. 17. https://doi.org/10.3390/molecules25173955
Watts BM, Ylimaki GL, Jeffery LE, Elias LG. 1991. Basic methods for the sensory evaluation of foods. IDRC, Ottawa, ON, CA.
Medoatinsa Seinde Esperance, Koudouro Yaya Alain, Olaye Theophile, Hounkanrin Fifame Judith, Kpatinvoh Brice, Bogninou G. Sophie Reine, Bothon F. T. Diane, Agbangnan Dossa Cokou Pascal (2024), Physico-chemical and microbiological characterizations of an herbal toothpaste formulated with extracts of some selected medicinal plant harvested in Benin Republic; IJB, V25, N1, July, P255-268
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