Welcome to International Network for Natural Sciences I INNSpub

Paper Details

Research Paper | May 1, 2020

| Download 1

Physiological and biochemical changes in Vigna unguiculata (L.) Walp. due to nickel stress

Atia Arzoo, Arpita Behera, Bhagyeswari Behera, Ashirbad Mohapatra, Kunja Bihari Satapathy

Key Words:

Int. J. Biosci.16(5), 70-76, May 2020

DOI: http://dx.doi.org/10.12692/ijb/16.5.70-76


IJB 2020 [Generate Certificate]


Environmental pollution due to industrial, mining and agricultural activity as well as transportation, leads to production of high amounts of contaminants like heavy metals into surface water and soils which ultimately leaches to ground water and also affects the biosphere. In the present investigation, germination study was conducted in cowpea [Vigna unguiculata (L.) Walp.] In order to find out the changes in germination, different developmental stages in terms of its growth, physiological and biochemical alteration due to nickel stress. The seeds of cow pea were germinated in variable nickel concentrations ranging from 0-100 mg/l of nickel. In another experiment conducted in pot culture revealed that, parameters the growth parameters and different physiological and biochemical parameters were decreased whereas only free proline content were increased with increase in concentration of nickel. It was also observed that seeds of cow pea showed better result in terms of growth and different physiological and biochemical parameters in 20 ppm of nickel at different interval of days like 10th, 20th and 30th day of growth of seedling thereby indicating that nickel within 20mg/kg could facilitate the plants growth and it is subsequently affect the seedling when it exceeds from 20 mg/kg of nickel in soil.


Copyright © 2020
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Physiological and biochemical changes in Vigna unguiculata (L.) Walp. due to nickel stress

Arnon DI. 1949. Copper enzymes in isolated chloroplasts polyphenol oxidase in Beta vulgaris. Plant Physiologist 24, 1-15. http://dx.doi.org/10.1104/pp.24.1.1

Arzoo A, Nayak SK, Mohapatra A, Satapathy KB. 2014. Impact of nickel on germination, seedling growth and biochemical changes of Macrotyloma uniflorum (Lam) Verdc. International Journal of Biosciences 5(9), 321-331.

Arzoo A, Satapathy KB. 2015. Physiological and biochemical responses induced by nickel to Arachis hypogea L. International Research Journal of Environmental Science 4(11), 19-24.

Baki AA, Anderson JD. 1973. Vigour determination in soybean seed by multiple criteria. Crop Science 3, 630-633. http://dx.doi.org/10.2135/cropsci1973.0011183X001300060013x

Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39, 205-208. http://dx.doi.org/10.1007/BF00018060

Biswal SK, Pradhan TM, Arzoo A. 2019. Response of Waste Egg Shells to Soil Fertility and Its Impacts on the Growth of Vigna mungo L. Seedling. Clay Research 38(1), 29-34.

Brown PH, Welch RM, Carry E. 1987. Nickel is a micronutrient essential for higher plants. Plant Physiolog 85, 801-803.

Chand S, Pandey A, Patra DD. 2012. Influence of nickel and lead applied in combination with vermicompost on growth and accumulation of heavy metals by Mentha arvensis Linn. cv Kosi. Indian Journal Natural Product Resource 3, 256-261.

Corradi MG, Blanchi A, Albasini A. 1993. Chromium toxicity in Salvia sclarea. Effects of hexavalent chromium on seed germination and seedling development. Environmental and Experimental Botany 33(3), 405-413. http://dx.doi.org/10.1016/0098-8472(93)90043-F

Duffus JH. 2002. Heavy metals a meaningless term? (IUPAC Technical Report), Pure and Applied Chemistry 74, 793-807. http//dx.doi.org/10.1351/pac.200274040793

Hall JL. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany 53, 1-11.

IARC. (International Agency for Research on Cancer). 1990. IARC Monograph on the evaluation of carcinogenic risks to humans 49, Lyans, France IARC 318-411.

Kalita MC, Devi P, Bhattacharya I. 1993. Effect of cadmium on seed germination, early seedling growth and chlorophyll content of Triticum aestivum. Indian Journal of Plant Physiology 36(3), 189-190.

Lowry OH, Rosenbrough NJ, Farr AL, Randall 1951. Protein measurement with Folin-Phenol reagent. Journal of Biological Chemistry 193, 265-275.

Mahalakshmi G, Vijayarengan P. 2003. Effects of zinc on germinating seeds of three plant species. Nature Environment and Pollution Technology 2(1), 117-119.

Pati S, Ghadei A, Arzoo A, Nayak SK, Mohapatra A, Satapathy KB. 2014. Physiological responses induced by chromium +6 toxicity to Cucumis sativus L. and Macrotyloma uniflorum Lam. IOSR Journal of Environmental Science, Toxicology and Food Technology 8 (12), 58-63.

Sadon FN, Ibrahem AS, Ismail KN. 2012. An overview of rice husk applications and modification techniques in wastewater treatment. Journal Purity Utility Reaction Environment 1, 308-334.

Sahoo RK. Nickel Ore. 1998. In: B.K. Mohanty (Ed.) Geology and Mineral Resources of Orissa. Society of Geoscientists and Allied Technologist, 323-338.

Singh BK, Walker A, Wright DJ. 2006. Bioremedial potential of fenamiphos and chlorpyriferous degrading isolates: Influence of different environment conditions. Soil Biology and Biochemistry 38, 2682-2693.

Wang GP. 2005. Study on impact and restoration of ecological environment of mining subsidence in Chongqing region. Journal of Chongqing University 10, 3-8.

Zhang LL. 2013. A certain mining area of Chongqing the inclined coal-seam mined causes surface movement deformation mechanism and geological environment problems. Chengdu University of Technology 6, 1-5.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background