Physiological and biochemical changes observed in alternative cellar model: Paramecuim tetraurelia treated with paracetamol
Paper Details
Physiological and biochemical changes observed in alternative cellar model: Paramecuim tetraurelia treated with paracetamol
Abstract
During their passage into the human or animal body, most of xenobiotics are metabolized. The metabolites formed are eliminated primarily through the kidneys and therefore are found in wastewater. Presence of synthetic molecules even at trace concentrations is an extremely pernicious pollution may lead to their accumulation in aquatic ecosystems. In this work, we focused particularly on the toxic potential of an antipyretic analgesic wide use: Paracetamol on an alternative cellular model: the protist ciliate Paramecium tetraurelia. The results show that the IC50 after 2h of exposure to xenobiotic is more 0.53μM; paramecia are sensitive to paracetamol and exhibit morphological and physiological changes and a strong disturbance of respiratory metabolism. In addition we have demonstrated a significant increase in GSH and MDA to confirm the toxicity of the tested compounds.
Acker V, Koymans LMH, Bast A.1993. Molecular pharmacological importance of vitamin E, structural aspects of NADP. Free radical biology & medicine 5, 311-328. http://dx.doi.org/10.1016/08915849(93)
Amanchi NR, Hussain MM. 2010. Cytotoxicity assessement of monocrotophos in Paramecium caudatum and Oxytricha fallax. Journal of Environmental Biology 31(5), 603-607.
Azzouz ZH. Berrebbah MR. Djebar. 2011. Optimization of Paramecium tetraurelia growth kinetics and its sensitivity to combined effects of azoxystrobin and cyproconazole. African Journal of Microbiology Research 5(20), 3243-3250. http://dx.doi.org/10.5897/AJMR11.322
Barry SR, Bernal J. 1993. Antimalarial drugs inhibit calcium-dependent backward swimming and calcium currents in Paramecium calkinsi. Journal of Comparative Physiology A 172, 457-466.DOI: 10.1007/s003590050064
Benbouzid H, Berrebah H, Berredjem M Djebar MR. 2012. Toxic effects of phosphoramidate on Paramecuim sp. With special emphasis on respiratory metabolism, growth, and generation time. Toxicological and Environmental Chemistry 94(3), 557-565. http://dx.doi.org/10.1080/02772248.2012.655696
Binelli A, Pedriali A, Riva C, Parolini M. 2012. Illicit drugs as new environmental pollutants: cyto- genotoxic effects of cocaine on the biological model Dreissena polymorpha. Chemosphere 86(9), 906–911. http://dx.doi.org/10.1016/j.chemosphere.2011.10.056
Bridger S, Henderson K, Glucksman E, Ellis AJ, Henry JA, Williams R.1998. Lesson of the week: Deaths from low dose paracetamol poisoning. British Medical Journal 316, (7146) 1724 – 1725. http://dx.doi.org/10.1136/bmj.316.7146.1724
Cadenas E, Boveris A, Ragan C.I. and Stoppani AOM. 1977. Production of superoxide radicals and hydrogen peroxide by NADH-ubiquinone reductase and ubiquinol cytochrome c reduction from beef heart mitochondria. Archives of Biochemistry and Biophysics 180, 248-257. http://dx.doi.org/10.1016/0003-9861(77)90035-2
Chagra A, Djebar MR, Rouabhi R, Berrebbah H. 2009. Calcium induced changes in Metabolic Function of Mitochondrial isolated from Potato Tissu (Solanum tubersome L.). American Journal of Biochemistry and Biotechnology 5(1), 35-39. http://dx.doi.org/10.3844/ajbbsp.2009.35.39
Coetsier C. 2009. Discharge of pharmaceutical products (PPs) through a conventional biological sewage treatment plant: MECs vs PECs. Environment International 35(5), 787–792. http://dx.doi.org/10.1016/j.envint.2009.01.008
Cohn J, Macphail RC. 1996. Ethological and experimental approaches to 1behavior analysis: implication for ecotoxicology, Environmental Health Perspectives. 104, 299-304.
Draper HH, Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 186, 241-431
Dybing E, Holme JA, Gordon WP, Soderlund EJ, Dahlin DD. and Nelson SD. 1984. Genotoxicity studies with paracetamol. Mutation Research 138, 21-32. http://dx.doi.org/10.1016/0165-1218(84)90081-8
Einicker-Lamas M, Mezian GA, Fernandes TB, -Silva FLS, Miranda K, Attia M, Oliveira MM. 2002. Euglena gracilis as a model for the study of Cu2+ and Zn2+ toxicity and accumulation in eukaryotic cells. Environment Pollution 120, 779-78. http://dx.doi.org/10.1016/S0269-7491(02)00170-7
Epstein SS, Burroughs M, Small M. 1963. The photodynamic effect of the carcinogen, 3,4-Benzpyrene, on Paramecium caudatum. Cancer Research 23 ,35-44.
Finney DJ. 1971. Probit Analysis, second ed., Cambridge University Press, Cambridge, London.
Friedl P, Wolf K. 2003. Tumour-cell invasion and migration: diversity and escape mechanisms, Nature Reviews Cancer 3, 362-374. http://dx.doi.org/10.1038/nrc1075
Grist EPM, O’Hagan A, Crane M, Sorokin N, Sims I, Whitehouse P. 2006. Bayesian and time-idependent species sensitivity distributions for risk assessment of chemicals. Environmental Science & Technology 40, 395-401. http://dx.doi.org/10.1021/es050871e
Hongslo JK, Bjorge C, Schwarze PE, Brogger A, Mann G, The lander L, Holme JA.1990. Paracetamol inhibits replicative DNA synthesis and induces sister chromatid exchange and chromosome aberrations by inhibition of ribonucleotide reductase. Mutagenesis 5, (5) 475-480. http://dx.doi.org/10.1093/mutage/5.5.475
Lahouel M, Ouadi O, Khiari N. 1996. Effets hépatique et hématologique du paracétamol. Saidal .7, 60–6.
McCormack K. 1994. Non-steroidal anti-inflammatory drugs and spinal nociceptive processing. Pain 59, 9-43. http://dx.doi.org/10.1016/0304-3959(94)90045-0
Mills JC, Stone NL, Erhardt J, Pittman RN. 1998. Apoptotic membrane blebbing is regulated by myosin light chain phosphorylation, The Journal of Cell Biology 140(3), 627-636. http://dx.doi.org/10.1083/jcb.140.3.627
Mofredj A, Cadranel JF, Darchy B, Barbare JC, Cazier A, PrasV, BM. 1999. Toxicité hépatique du paracétamol à dose thérapeutique chez le sujet éthylique chronique (à propos de deux cas d’hépatite mortelle chez des patients cirrhotiques). Annales de Médecine Interne 150, 507–11.
Mohd M, Hussain. 2008. Low cost microbioassay test for assessing cytopathological and physiological responses of ciliate model Paramecium caudatum to carbofuran .Pesticide Biochemistry and Physiology (2), 66–70. http://dx.doi.org/10.1016/j.pestbp.2007.07.006
O’Grady JG. 1997. Paracetamol-induced acute liver failure: prevention and management. Clinical journal of gastroenterology 26 suppl1:41–6.
Rouabhi R, Djebar H and Djebar MR. 2006. Effect of diflubenzuron on the cellular model, Paramecium sp. African Journal of Biotechnology, 5(1), 045-048.
Sako F, Taniguchi N, Kobayashi E, Takakuwa. 1977. Effects of food days on Paramecium caudatum: toxicity and inhibitory effects on leucine aminopeptidase activity, Toxicology and Applied Pharmacology 39,111-117. http://dx.doi.org/10.1016/0041-008X(77)90183-1
Sauvant NP, Pepin D, and Piccinni E . 1999. Tetrahymena pyriformis: A tool for toxicological studies. Chemosphere, 38(7), 1631-1669. http://dx.doi.org/10.1016/S0045-6535(98)00381-6
Sbartai I, Berrebah H, Rouabhi R, Sbartai H, Guy S and Djebar MR. 2009. Behavior of Paramecium sp., Treated with Bifenazote with Special emphasis on Respiratory Métabolism, Protein and Generation Time. American-Eurasian Journal of Toxicological Science 1(1), 13-18.
Scherrer E. 1992. Behavioral responses as indicator of environmental alterations: approaches, results, developments, Journal of Applied Ichthyology 8, 122-131. http://dx.doi.org/10.1111/j.1439-0426.1992.tb00674.x
Settaf M, Zahidy A, Elimadi R, Sapena I, Abd A, Tillement J-P, et al. 2000. S-15176 reduces the hepatic injury in rats subjected to experimental ischemia and reperfusion. European Journal of Pharmacology, 406, 281–292. http://dx.doi.org/10.1016/S0014-2999(00)00599-9
Venkateswara JR, Gunda VG, srikanth K, Arepalli Sk. 2007. Acute toxicity bioassay using Paramecium Caudatum, a key member to study the effects of monocrotophos on swimming behavior, morphology and reproduction. Toxicological & Environmental Chemistry 89, 307-317. http://dx.doi.org/10.1080/02772240601010071
Venkateswara JR, Srikanth K, Arepalli SK, Gunda VG. 2006. Toxic effects of acephate on paramecium caudatum with special emphasis on morphology, behaviour and generation time. Pesticide Biochemistry and Physiology 86, 131-137. http://dx.doi.org/10.1080/02772248
Venkateswara JR., Arepalli SK Gunda VG. Kumar BJR. 2008. Assessment of cytoskeletal damage in paramecium caudatum: An early warning system for apoptotic studies. Pesticide Biochemistry and Physiology 91, 75-85 http://dx.doi.org/10.1016/j.pestbp.2008.01.004
Weckberker G, Cory JG. 1988. Ribonucléotide reductase activity abd growth of glutathione depleted mouse leukemial 1210 cells in vitro. Cancer letters 40, 257-264. http://dx.doi.org/10.1016/0304-3835(88)90084-5
Wong CK, Cheung Ming-Ho Yo. 1999. Toxicological assessement of coastal sediments in Hong Kong using a flagellate Dunalliella tertiolecta. Environmental pollution 105, 175-183. http://dx.doi.org/10.1016/S0269-7491(99)00027-5
Boulassel Amina, Djebar Mohamed Réda, Rouabhi Rachid, Djebar Houria (2013), Physiological and biochemical changes observed in alternative cellar model: Paramecuim tetraurelia treated with paracetamol; IJB, V3, N9, September, P132-141
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