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Phytase supplementation improves growth performance and crude protein digestibility in Labeo rohita fingerlings fed barley meal based diet

Research Paper | June 1, 2017

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Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah, Syed Makhdoom Hussain

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J. Bio. Env. Sci.10( 6), 124-132, June 2017


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Barley is a staple cereal grain, full of digestible proteins and energy, can be effectively used to replace fishmeal. Phytase is an enzyme used to hydrolyze phytate (anti-nutrient) present in plant proteins. Therefore, this study was designed to evaluate the optimum level of phytase supplementation for maximum growth and nutrient digestibility in Labeo rohita fingerlings fed barley meal based diet. Fish (average body weight 14.28±0.14 g) were fed on 8 experimental diets including 1 reference and 7 test diets. Reference diet was used as standard diet and formulated to provide all necessary nutrients required for normal fish growth. Test diets were consisted of 70% reference and 30% barley meal and were supplemented with graded levels of phytase (0, 250, 500, 750, 1000, 1250, 1500 FTUkg-1). Chromic oxide was used as inert marker to assess nutrient digestibility. Fingerlings having 750 FTU kg-1 phytase supplemented diet showed significantly (p<0.05) improved growth and feed performance as compared to reference and other test diets. Similarly, digestibility data also showed maximum absorption of crude protein and gross energy at 750 FTUkg-1 phytase level. In conclusion, phytase supplementation at the level of 750 FTUkg-1diet can improve growth performance and nutrient digestibility of L. rohita fingerlings to its maximum level in barley meal based diet. However, higher levels of its supplementation were not helpful to further improve the growth and digestibility performance.


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