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Phytochemical Identification and Comparative Antifungal Efficacy of Dodonaea viscosa L. Fractions against Ceratocystis manginecans

Research Paper | December 1, 2021

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Kokab Jabeen, Shahzad Asad

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Int. J. Biosci.19( 6), 110-118, December 2021

DOI: http://dx.doi.org/10.12692/ijb/19.6.110-118


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Mango Sudden Death (MSD) is caused by Ceratocystis manginecans. It is an important disease distressing mango production in major mango growing countries of the world. Synthetic fungicide applications help to control MSD but biological control of MSD can be eco-friendly and economically efficient approach. Current study was intended to assess antifungal potential of botanical fractions of Dodonaea viscosa L. against Ceratocystis manginecans. Liquid extraction of crude ethanol extracts of D. viscosa was performed using four solvents with an increasing polarity order i.e. n-Hexane, Dichloromethane (DCM), Ethyl Acetate (EA) and n-Butanol. In-vitro Antifungal potential assessment by poisoned food technique revealed that Ethyl Acetate fraction (40mg/ml) exhibited highest potential (75.178%) to control growth of C. manginecans. MIC value of Ethyl Acetate fraction was recorded 8mg/ml. Phytochemical analysis of Ethyl Acetate fraction through GCMS identified presence of Oleoyl Chloride (C18H33ClO), Octadecanoic Acid (C155H106O6), Oleic Acid (C24H44O4), [I-(+)-Ascorbic Acid 2,6 Hexadecanoate] (C38H68O8), [(9E)-9-Octadecanoic Acid] (C18H34O2), [9,12-Octadecadienoic Acid (Z,Z)] (C18H32O2), [1,2-Oxathiane, 6-Dodecyl-, 2,2-Dioxide] (C16H32O3S), and 1,2-Butanediol (C19H14O3). These phytochemical compounds of Ethyl Acetate fraction potentially contributed to control C. manginecans growth. According to our knowledge this is first study to explore fractions of D. viscosa ethanol crude extract for the control of C. manginecans.


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Phytochemical Identification and Comparative Antifungal Efficacy of Dodonaea viscosa L. Fractions against Ceratocystis manginecans

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