Phytochemical screening and antibacterial activity of green synthesis of nanoparticles of Punica granatum

Paper Details

Research Paper 01/07/2020
Views (767)
current_issue_feature_image
publication_file

Phytochemical screening and antibacterial activity of green synthesis of nanoparticles of Punica granatum

Taufiq Nawaz, Hamid Ali, Muhammad Imran, Sheraz Ahmed, Sana Noreen, Bahisht Rizwan, Muhammad Qumar, Faiza Iftikhar, Hamna Ahmed, Sana Farooq, Fatima Syed
Int. J. Biosci. 17(1), 160-166, July 2020.
Copyright Statement: Copyright 2020; The Author(s).
License: CC BY-NC 4.0

Abstract

The main objective of this work was to explore Punica granatum phytochemicaly and synthesize nanoparticles from its polar extract. The phytochemical screening test of ethanolic and aqueous extract of P. granatum indicated the presence of tannins, flavonoides, emodins, terpenoids, cardiac glycosides, coumarine, and soluble starch. The aqueous extract showed the presence of tannins, flavonoides, terpenoids, emodins, cardiac glycosides, caumarine, carbohydrates and soluble starch. The extracts were subjected to synthesize gold nanoparticles (Au-NPs) Synthesized Au-NPs were characterized by using Uv-visible, Fourier transform (FT-IR) spectroscopy and AFM (atomic force microscope) analysis. The active phytochemical present in extract are responsible for synthesizes gold nanoparticles (Au-NPs). In addition, synthesized Au-NPs and the methanolic extracts of Punica granatum were screened for their in-vitro antioxidant activity. Au-NPs showed excellent antioxidant potentials. It is concluded that Punica granatum extracts is an outstanding bioreducant for the rapid and green synthesis of Au-NPs, which in turn showed various antioxidant activity.

Chidambara Murthy KN, Jayaprakasha GK, Singh RP. 2002. Studies on antioxidant activity of pomegranate (Punica granatum) peel extract using in vivo models. Journal of Agricultural and Food Chemistry 50(17), 4791-4795.

Daniel MC, Astruc D. 2004. Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chemical reviews 104(1), 293-346.

Hossain Z, Yasmeen F, Komatsu S. 2020. Nanoparticles: Synthesis, Morphophysiological Effects, and Proteomic Responses of Crop Plants. International Journal of Molecular Sciences 21(9), 3056.

Lee MJ, Jang M, Choi J, Chang BS, Kim SH, Kwak YS, Cho IH. 2016. Korean red ginseng and ginsenoside-Rb1/-Rg1 alleviate experimental autoimmune encephalomyelitis by suppressing Th1 and Th17 cells and upregulating regulatory T cells. Molecular neurobiology 53(3), 1977-2002.

Murad W, Ahmad A, Ishaq G, Saleem Khan M, Muhammad Khan A, Ullah I, Khan I. 2012. Ethnobotanical studies on plant resources of Hazar Nao forest, district Malakand, Pakistan. Pakistan Journal of Weed Science Research 18(4).

Philip D, Kaleena PK, Valivittan K, Kumar CG. 2011. Phytochemical screening and antimicrobial activity of Sansevieria roxburghiana Schult. and Schult. F. Middle-East Journal of Scientific Research 10(4), 512-8.

Rafieian-Kopaei M. 2013. Medicinal plants and the human needs. Journal of Herb Med Pharmacology 1(1).

Rauf A, Muhammad N, Khan A, Uddin N, Atif M. 2012. Antibacterial and phytotoxic profile of selected Pakistani medicinal plants. World Applied Sciences Journal 20(4), 540-544.

Rose EA, Gelijns AC, Moskowitz AJ, Heitjan DF, Stevenson LW, Dembitsky W, Watson JT. 2001. Long-term use of a left ventricular assist device for end-stage heart failure. New England Journal of Medicine 345(20), 1435-1443.

Schippmann U, Leaman DJ, Cunningham AB. 2002. Impact of cultivation and gathering of medicinal plants on biodiversity: global trends and issues. Biodiversity and the ecosystem approach in agriculture, forestry and fisheries.

Seebacher W, Simic N, Weis R, Saf R, Kunert O. 2003 Complete assignments of 1H and 13CnmR resonances of oleanolic acid, 18α‐oleanolic acid, ursolic acid and their 11‐oxo derivatives. Magnetic Resonance in Chemistry 41(8), 636-638.

Seniya C, Trivedia SS, Verma SK. 2011. Antibacterial efficacy and phytochemical analysis of organic solvent extracts of Calotropis gigantea. Journal of Chemical and Pharmaceutical Research 3(6), 330-336.

Shahrajabian MH, Sun W, Cheng Q. 2019. Traditional Chinese medicine and agriculture; organic life and sustainability for future. GSC Biological and Pharmaceutical Sciences 7(1), 091-095.

Sultana ARSHIYA, Rahman KHALEEQUR. 2013. Portulaca oleracea Linn. A global Panacea with ethno-medicinal and pharmacological potential. International Journal of Pharmacy and Pharmaceutical Sciences 5, 33-39.

Uddin G, Rauf A, Rehman TU, Qaisar M. 2011. Phytochemical screening of Pistacia chinensis var. integerrima. Middle-East Journal of Scientific Research 7(5), 707-711.

Related Articles

Muscle type and meat quality of local chickens according to preslaughter transport conditions and sex in Benin

Assouan Gabriel Bonou*, Finagnon Josée Bernice Houéssionon, Kocou Aimé Edenakpo, Serge Gbênagnon Ahounou, Chakirath Folakè Arikè Salifou, Issaka Abdou Karim Youssao, Int. J. Biosci. 27(6), 241-250, December 2025.

Effects of micronutrients and timing of application on the agronomic and yield characteristics of cucumber (Cucumis sativus)

Princess Anne C. Lagcao, Marissa C. Hitalia*, Int. J. Biosci. 27(6), 214-240, December 2025.

Response of different soybean varieties to phosphorus fertilizer microdosing and rhizobium inoculation in the sub-humid zone of Northern Benin

Pierre G. Tovihoudji*, Kamarou-Dine Seydou, Lionel Zadji, Sissou Zakari, Valerien A. Zinsou, Int. J. Biosci. 27(6), 201-213, December 2025.

On-farm validation of black soldier fly larvae meal as a sustainable replacement for shrimp meal in rainbow trout diets in the mid hills of Nepal

Ishori Singh Mahato, Krishna Paudel*, Sunita Chand, Anshuka Bhattarai, Int. J. Biosci. 27(6), 189-200, December 2025.

Insect fauna associated with Cucumis sativus (Cucurbitales: Cucurbitaceae) in Parakou, A cotton-growing area of central Benin

Lionel Zadji*, Mohamed Yaya, Roland Bocco, Prudencia M. Tovignahoua, Abdou-Abou-Bakari Lassissi, Raphael Okounou Toko, Hugues Baimey, Leonard Afouda, Int. J. Biosci. 27(6), 175-188, December 2025.

First record of two hymenopteran species, Brachymeria excarinata Gahan (Chalcididae) and Pteromalus sp. (Pteromalidae), as hyperparasitoids of Diadegma insulare in Senegal

Babacar Labou*, Etienne Tendeng, Mamadou Diatte, El hadji Sérigne Sylla, Karamoko Diarra, Int. J. Biosci. 27(6), 167-174, December 2025.

Hepatoprotective and antinociceptive effects of terpinolene in streptozotocin-induced diabetic peripheral neuropathic rats

Ravishankar Sarumathi, Muthukumaran Preethi, Chandrasekaran Sankaranarayanan*, Int. J. Biosci. 27(6), 156-166, December 2025.