Phytochemical screening and antitumor potential of Punica granatum peel extract

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Research Paper 01/10/2015
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Phytochemical screening and antitumor potential of Punica granatum peel extract

Wasim Sajjad, Nikhat ilahi, Muhammad Hayat, Faisal Ahmad, Zia Ur Rahman
Int. J. Biosci.7( 4), 102-110, October 2015.
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The constant investigation on medicines are vital to resolve growing complication in medical conditions and microbes resistance. Medicinal plants such as Punica granatum are storehouse of certain valuable phytochemical substances that might be helpful in medical complications like tumor. The current research was aimed to study the phytochemistry of P. granatum peel extract and explore In vitro antitumor activity of extracts prepared in different solvents including ethanolic, methanolic, aqueous, ethyl acetate and petroleum ether. Qualitative study for the existence of certain phytochemical compounds like steroids, saponins, alkaloids, carbohydrates, phlobatanins, anthraquinones, glycosides and tannins was accomplished for each extract using standard methods. Most of the phytochemical molecules were present in methanolic, ethanolic and ethyl acetate extracts while least number of molecules were found in aqueous solvent. The antitumor activity was examined by using Agrobacterium tumefaciens At 10 strain and potato disc tumor assay technique and assessment were established on the basis of tumor inhibition of several concentration of extracts (10 ppm, 100 ppm, 1000 ppm). Results discovered that different concentrations of P. granatum peel extract showed different percentage of inhibition. Increase in percentage inhibition of tumor were observed with increase of peel extract concentration. At 1000 ppm of crude ethanolic extract (CEE) showed maximum inhibition 65%. All the extracts significantly inhibited tumor formation. The obtained results showed antitumor efficacy of P. granatum peel extracts.


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