Phytochemical screening and larvicidal efficacy of Eupatorium capillifolium (Dog-fennel) against Aedes aegypti and Culex quinquefasciatus

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Research Paper 01/11/2017
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Phytochemical screening and larvicidal efficacy of Eupatorium capillifolium (Dog-fennel) against Aedes aegypti and Culex quinquefasciatus

Reverie R. Barrios, Glaiza Sofia B. Garcia, Milton Norman D. Medina, Normeliza Morales
J. Bio. Env. Sci.11( 5), 385-396, November 2017.
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Abstract

The primary purpose of this study is to evaluate the larvicidal efficacy of Eupatorium capillifolium (Dog-fennel) crude extract on Aedes aegypti and Culex quinquefasciatus. Phytochemical constituents were identified using qualitative screening. All tests were done at the Microbiology Laboratory of the University of Mindanao, Matina, Davao City. One-way ANOVA was use in analyzing the data. Phytochemical screening of the crude extract revealed the presence of tannins, alkaloids, tri-terpenoids, coumarins, saponins, steroid and phytosteroid, flavanones, flavones, carbohydrates and amino acids. A total of 1,200 3rd and 4th instars larvae of A. aegypti and C. quinquefasciatus were exposed to different concentrations of control treated with acetone and plant extracts ranging from 25%-100%. Larval mortality was observed after 1, 2, 6, 12, and 24 hours exposures. Highest susceptibility and toxicity with 99% was recorded after 1 hour exposure in 50%, 75% and 100 % concentrations of E. capillifolium. The larvae of A. aegypti revealed 86.68 % mortality rate in the plant extract while 48.7 % in control. Meanwhile, C. quinquefascatus showed 91% mortality rate in the extract and 33.3% in control. This indicates that crude leaf extract of E. capillifolium is very effective in exterminating A. aegypti and C. quinquefasciatus larvaes. This finding may lead to a possible new low cost alternative and environmentally friendly method for mosquito control programs.

VIEWS 8

Abayomi S. 1993. Historical review of traditional medicine in Africa. Ibadan, Nigeria: Spectrum Limited, 925.

Bisht SS, Kamal R. 1994. Garlic extract: An antifungal treatment for the control of storage of apple. Proceedings of the National Academy of Sciences 64, 233-234.

Cho JY. 2006. Sesquiterpene lactones as a potent class of NF-KB activation inhibitors. Current Enzyme Inhibition 2(4), 329-341.

Conner WE, Boada R, Schroeder FC, Gonzalez A, Meinwald J, Eisne, T. 2000. Chemical defense: Bestowal of a nuptial alkaloidal garment by a male moth on its mate. Department of Biology, Wake Forest University, P. O. Box 7325, Winston-Salem, NC 27109; and Departments of †Chemistry and Chemical Biology and Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.

Dharmananda S. 2002. Safety issues affecting herbs: Pyrrolizidine alkaloids. Traditional Medicine 3, 217-218.

Geiss F. 1987. Fundamentals of thin layer chromatography. Heidelberg, Germany: VCH Publishing.

Guevara BQ. 2004. A guidebook to plant screening: Phytochemical and biological. Manila: UST Publishing House.

Haddad LM, Winchester JF. 1990. Clinical management of poisoning and overdose. Philadelphia, PA: W.B. Saunders Company.

Hamid HK, Kadhi EJ. 2016. Extraction, isolation and characterization of pyrrolizidine alkaloids present in Senecio vulgaris Linn. grown in Iraq. Journal of Pharmacognosy and Phytochemistry 5(6), 28-37.

Harborne AJ. 2005. Phytochemical methods a guide to modern techniques of plant analysis. The Netherlands: Springer 412-572.

Hill S, Connelly CR. 2009. Southern House Mosquito Culex quinquefasciatus Say. EENY-457. University of Florida, IFAS Extension, 1-5.

Kellerman TS, Fourie N, Naude TW. 1996. Plant poisonings and mycotoxicoses of livestock in Southern Africa. Cape Town: Oxford University Press.

King RM, Robinson H. 1987. The genera of Eupatorieae (Asteraceae). Monographs in systematic botany. Missouri Botanical Garden 22, 1-581.

Manimegalai K, Sukanya S. 2014. Biology of the vector Culex quinquefasciatus Diptera: Culicidae). International Journal of Current Microbiology and Applied Sciences 3(4), 718-724.

Modzelewska A, Sur S, Kumar SK, Khan SR. 2005. Sesquiterpenes: Natural products that decrease cancer growth. Current Medicinal Chemistry and Anti-Cancer Agents Journal 5(5), 477-99.

Ober D, Hartmann T. 1999. Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase. Institute of Pharmaceutical Biology 1, 38106.

Rathy MC, Sajith U, Harilal CC. 2015. Larvicidal efficacy of medicinal plant extracts against the vector mosquito Aedes albopictus. International Journal of Mosquito Research 2(2), 80-82.

Schmidt GJ, Schilling EE. 2000. Phylogeny and biogeography of Eupatorium (Asteraceae: Eupatorieae) based on nuclear ITS sequence data. American Journal of Botany 87, 716-726.

Shaalan EAS, Canyonb D, Younesc MWF, Abdel-Wahaba H, Mansoura AH. 2005. A review of Botanical Phytochemicals with Mosquitocidal Potential. Environment International Journal 3, 1149–66.

Sugumar N, Karthikeya S, Gowdhami T. 2014. Chemical Composition and Antimicrobial Activity of Essential Oil from Eupatorium triplinerve Vahl. Aerial Parts. International Letters of Natural Sciences 31, 14-21.

Sugumar N, Karthikeyan S, Gowdhami T. 2015. Phytochemical analysis and antimicrobial activity of Eupatorium triplinerve Vahl. International Journal of Applied Research 1(12), 108-112.

Sukumar K, Perich MJ, Boobar LR. 1991. Botanical derivatives in mosquito control: A review. Journal of Mosquito Control 7, 210–37.

Tabanca N, Bernier UR, Tsikolia M, Becnel JJ, Sampson B, Werle C, Demirci B, Husnu K, Blythes EK, Pounders C, Wedge DE. 2010. Eupatorium capillifolium Essential Oil: Chemical Composition, Antifungal Activity, and Insecticidal Activity. Natural Product Communications 5(9), 1409-1415.

Vinogradova EB, Fedorova MV, Shaikevich EV, Zakharov IA. 2003. Endosymbiotic bacteria Wolbachia pipientis in synanthropic populations of mosqioto Culex pipiens L. (Diptera, Culicidae). Journal of Russian Academic Science 389 (6), 1-5.

World Health Organization. 1992. WHO commission on health and environment. Report of the panel on urbanization.

World Health Organization. 1996. Report of the WHO Information Consultation on the Evaluation and Testing of the Insecticidal Control of Tropical Diseases Division, WHO Publication. CTD/WHOPES/I.C Geneva pp. 69.

World Health Organization. 1998. The WHO recommended classification of pesticides by hazard and guidelines for classification 1998–1999. Geneva, International Programme on Chemical Safety/World Health Organization.

World Health Organization. 2005. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. WHO/CDS/ WHOPES/GCDPP/2005.13, pp. 10–11.