Phytochemical screening and larvicidal efficacy of Eupatorium capillifolium (Dog-fennel) against Aedes aegypti and Culex quinquefasciatus

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Research Paper 01/11/2017
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Phytochemical screening and larvicidal efficacy of Eupatorium capillifolium (Dog-fennel) against Aedes aegypti and Culex quinquefasciatus

Reverie R. Barrios, Glaiza Sofia B. Garcia, Milton Norman D. Medina, Normeliza Morales
J. Bio. Env. Sci.11( 5), 385-396, November 2017.
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The primary purpose of this study is to evaluate the larvicidal efficacy of Eupatorium capillifolium (Dog-fennel) crude extract on Aedes aegypti and Culex quinquefasciatus. Phytochemical constituents were identified using qualitative screening. All tests were done at the Microbiology Laboratory of the University of Mindanao, Matina, Davao City. One-way ANOVA was use in analyzing the data. Phytochemical screening of the crude extract revealed the presence of tannins, alkaloids, tri-terpenoids, coumarins, saponins, steroid and phytosteroid, flavanones, flavones, carbohydrates and amino acids. A total of 1,200 3rd and 4th instars larvae of A. aegypti and C. quinquefasciatus were exposed to different concentrations of control treated with acetone and plant extracts ranging from 25%-100%. Larval mortality was observed after 1, 2, 6, 12, and 24 hours exposures. Highest susceptibility and toxicity with 99% was recorded after 1 hour exposure in 50%, 75% and 100 % concentrations of E. capillifolium. The larvae of A. aegypti revealed 86.68 % mortality rate in the plant extract while 48.7 % in control. Meanwhile, C. quinquefascatus showed 91% mortality rate in the extract and 33.3% in control. This indicates that crude leaf extract of E. capillifolium is very effective in exterminating A. aegypti and C. quinquefasciatus larvaes. This finding may lead to a possible new low cost alternative and environmentally friendly method for mosquito control programs.


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