Phytochemical screening, evaluation of antioxidant and antimicrobial properties of Erythrophleum ivorense A. Chev (Leguminosae) and Megaphrynium macrostachyum Benth (Marantaceae), Medicinal plants from Gabon

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Phytochemical screening, evaluation of antioxidant and antimicrobial properties of Erythrophleum ivorense A. Chev (Leguminosae) and Megaphrynium macrostachyum Benth (Marantaceae), Medicinal plants from Gabon

Sima Obiang Cédric, Ondo Joseph-Privat, Obame Engonga Louis-Clément, Padzys Guy-Stéphane, Zongo Cheikna, BONGUI Jean-Bernard, Nsi Emvo Edouard, Traore Alfred
Int. J. Biosci.8( 6), 43-53, June 2016.
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Abstract

Medicinal plants are used worldwide as an alternative and/or a complementary medicine, thus the valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. The water, water-ethanol and ethanol extracts of Erythrophleum ivorense and Megaphrynium macrostachyum, were evaluated for antimicrobial and antioxidant activities. The powdered plants samples were analyzed for their phytochemical screening using standard laboratory methods. The total phenols, flavonoid and antioxidant activities were evaluated with methods of the Folin-Ciocalteu, aluminum chloride and Antioxidant Activity Index (AAI) assay, respectively. The antimicrobial activity was evaluated by analyses of diffusion and micro dilution. The phytochemical analysis highlights the presence of total polyphenols and the flavonoids in the extracts of Erythrophleum ivorense compared to the extracts of Megaphrynium macrostachyum. Water, water-ethanol and ethanol extracts of Erythropleum ivorense showed a strong antioxidant activity (AAIWE=1.58; AAIWEE = 2.87; AAIEE = 2.38). The extracts of Megaphrynium macrostachyum have antimicrobial and antioxidant activities weak, compared to the extracts of Erythrophleum ivorense, Vitamin C and BHA. The use of these plants in traditional medicine is justified and they constitute a source for other traditional investigations.

VIEWS 10

Adjanohoum JE, Aké-Assi L, Floret JJ, Guinko S, Koumaré M, Ahyi AM, Raynal J. 2001. Médicine traditionnelle et Pharmacopée Contribution aux études ethnobotaniques et floristiques au Mali. ACCT, Paris. p. 291.

Arvouet-Grant A, Vennat B, Pourrat A, Legret P. 1994. Standardisation d’un extrait de propolis et identification des principaux constituants. Journal de la Pharmacology Belge 49(6), 462-468.

Andzi Barhé T, Massala KK, Obame LC, Lebibi J. 2015. Phytochemical studies, total phenolic and flavonoids content and evaluation of antiradical activity of the extracts of the leaves from Dischistocalyx sp. (Acanthacées). Journal of Pharmacognosy and Phytochemistry 3(6), 174-178.

Bors W, Hester W, Michel C, Saran M. 1990. Flavonoids as antioxidants, determination of radical scavenging efficiencies. Methods in Enzymology 186, 343-355. http://dx.doi.org/10.1016/0076-6879(90)86128-1

Bruneton J. 2009. Pharmacognosie, Phytochimie, plantes médicinales. In: EM inter, ed. Tec & Doc, 4ème édition.

Burkill HM. 1994. Useful plants of West Tropical Africa. Vol. 2. Families E-I. Royal.

Cicerale S, Lucas L, Keast R. 2010. Biological activities of phenolic compounds present in virgin olive oil. International Journal of Molecular Sciences 11(2), 458-479. http://dx.doi.org/10.3390/ijms11020458

Culei I. 1982. Methodology for the analysis of vegetable drugs. Practical manual on the industrial utilization of medicinal and aromatic plants. Center Building, Romania, 67-81.

Daniyan SY, Oloruntimelehin JB, Ifeadi O. 2011. Antibacterial activity of Cassia occidentalis flower vegetable extract on selected bacteria. Asian Journal of Biomedical and Pharmaceutical Sciences 1(1), 23-27.

Harborne JB, Williams CA. 2000. Advances in flavonoid research since 1992. Phytochemistry 55(6), 481-504. http://dx.doi.org/10.1016/S0031-9422(00)00235-1.

Iwu MW, Duncan AR, Okunji CO. 1999. New Antimicrobials of Plant origin. In: J.Janick, ed. Perspectives on new crops and new uses. ASHS Press, Alexandria, VA, 457-462.

Koudou J, Obame LC, Edou P, Bassolé I, Eba F, Figueredo G, Traoré AS, Chalchat JC. 2008. Volatile constituents, antioxidant and antibacterial properties of Daniella Klainei Pierre ex A. Chev. essential oil. Scientific Research and Essays 3, 316-319.

Le Minor L, Véron M. 1982. Bactériologie médicale. Flammarion médecine-sciences.

Lennette EH, Truant JP, Spaulding CH, Abella C. 1981. Manual de microbiología clínica. Salvat Editores.

Mandalari G, Bennett RN, Bisignano G, Trombetta D, Saija A, Faulds CB, Gasson MJ, Nabad A. 2007. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry. Journal of Applied Microbiology 103(6), 2050-2064. http://dx.doi.org/10.1111/j.1365-2672.2007.03456.x

Marc T, Gerard W, Denis L. 2001. Classification des anti-inflammatoires en guide de pharmacologie. Etudiants et professionnels paramédicaux. 4ème Edition. p 426.

NCCLS (National Committee for Clinical Laboratory Standards). 1999. Performance standards for antimicrobial susceptibility test. Ninth International Supplement. M100-S9. Edited by Standards) NNCfCL. Wayne, PA:

NCCLS (National Committee for Clinical Laboratory Standards). 2000. Methods for dilution, antimicrobial susceptibility tests for bacteria that grow aerobically. 5th edition volume 17. Approuved standards-M7-A4. NCCLS document M7-A4.

Ondo JP, Obame LC, Andzi BT, Nsi Akoué G, Nsi Emvo E, Lebibi J. 2013. Phytochemical screening, total phenolic content and antiradical activity of Asplenium africanum (Aspleniaceae) and fruit of Megaphrinium macrostachyum (Marantaceae).  Journal  of  Applied  Pharmaceutical Science 3(8), 092-096. http://dx.doi.org/10.7324/JAPS.2013.3816

Obame LC, Koudou J, Chalchat JC, Bassolé I, Edou P, Ouattara AS, Traoré AS. 2007. Volatile components, antioxidant and antibacterial activities of Dacryodes buettneri HJ Lam. essential oil from Gabon. Scientific Research and Essays 2, 491-495.

Pérez FJ, Ormeño–Nuñez J. 1991. Root exudates of wild oats. allelopathic effect on spring wheat. Phytochemistry 30, 2199-2202. http://dx.doi.org/10.1016/0031-9422(91)83614-Q.

Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C. 2000. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology 72(1-2), 35-42. http://dx.doi.org/10.1016/S0378-8741(00)00196-3

Raponda-Walker A, Sillans R. 1961. Plantes utiles du Gabon. Edition Lechevalier, Sepia, p 614.

Richter HG, Dallwitz MJ. 2000. Commercial timbers: descriptions, illustrations, identification, and information retrieval. In English, French, German, Portuguese, and Spanish.

Sabu M, Kattan R. 2002. Antidiabetic activity of medicinal plants and its relationship with their antioxidant property. Journal of Ethnopharmacology 81, 155–60. http://dx.doi.org/10.1016/S0378-8741(02)00034-X

Scherer R, Godoy HT. 2009. Antioxidant activity index (AAI) by 2, 2-diphenyl-1-picrylhydrazyl method. Food Chemistry 112, 654-658. http://dx.doi.org/org/10.1016/j.foodchem.2008.06.0 26

Sima Obiang C, Obame Engonga LC, Ondo JP, Zongo C, Nsi Emvo E, Traoré SA. 2015. Ethnotherapy study, phytochemical screening and antioxidant activity of Antrocaryon klaineanum Pierre and Anthocleista nobilis G. Don. Medicinal plants from Gabon. International Journal of Advanced Research 3(5), 812-819.

Sofowora A. 1993. Medicinal plants and traditional medicine in Africa. 2nd Edn. Spectrum Books limited, Ibadan, Nigeria, 1-153.

Trease GE, Evans WC. 2002. Pharmacognosy. 15th Edn. Saunders, 214-393.

Vernon  LS,  Orthofer  R,  Lamuela-Raventos RM. 1999.  Analysis of  total  phenols  and  other oxidation substrates and antioxidants by means of Folin-Ciocalteu  reagent. Methods  in  Enzymology 299, 152-178. http://dx.doi.org/10.1016/S0076-6879(99)99017-1

White LJT, Abernethy K. 1996. Guide de la végétation de la réserve de la Lopé, Gabon. Ecofac-Gabon, Libreville p 224.

WHO. 1995. The World Health Report. Bridging the Gaps. WHO, Geneva. 1-118.

Xia EQ, Deng GF, Guo YJ, Li HB. 2010.  Biological activities of polyphenols from grapes. International Journal of Molecular Sciences 11(2), 622-646. http://dx.doi.org/10.3390/ijms11020622

Yokozawa T, Dong EB, Kawai Y, Gemba M, Shimizu M. 1999. Protective effects of some flavonoids on the renal cellular membrane. Experimental and Toxicologic Pathology 51(1), 9-14. http://dx.doi.org/10.1016/S0940-2993(99)80050-5

Zongo C, Savadogo A, Ouattara L, Bassolé IHN, Ouattara CAT, Ouattara AS, Barro N, Koudou J, Traoré AS. 2010. Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch. (Vitaceae), a medicinal plant from Burkina Faso. International Journal of Pharmacology 6(6), 880-887. http://dx.doi.org/10.3923/ijp.2010.880.887