Phytoconstituents of Cassia auriculata Linn inhibits the inflammatory enzymes (5-LOX, COX-1 and COX-2): An insilico study to identify anti-inflammatory drug candidates

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Research Paper 03/04/2024
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Phytoconstituents of Cassia auriculata Linn inhibits the inflammatory enzymes (5-LOX, COX-1 and COX-2): An insilico study to identify anti-inflammatory drug candidates

Tejaswini Hipparagi, Shivaleela Biradar, Babu R.L
Int. J. Biosci.24( 4), 24-36, April 2024.
Certificate: IJB 2024 [Generate Certificate]

Abstract

Inflammation is the most essential part of body’s immune system and responsible for diseases manifestation in asthma, rheumatoid arthritis, allergy, aging, autoimmune diseases and etc.  During of these conditions, many cytokines were regulated. The pro-inflammatory cytokines perform an essential function in development of an effective defense against disease infections and progression. Along with cytokines many enzymes play a critical role in regulation of inflammation. The enzymes such as 5-LOX, COX-1 and COX-2 are vital targets. Objective of study is to comprehensive screening of highly potential and precise phytoconstituents from ethnomedicinally important Cassia auriculata Linn which may have potential inhibitors for pro-inflammatory targets. Methanol extract of Cassia auriculata Linn leaf was subjected to HR-LCMS analysis. The phytochemical signature was analyzed and 85 phytochemicals are taken for the molecular docking studies with pro-inflammatory markers. After, through screening 10 compounds were showing good binding energy with hydrogen bond interaction with all three targets. Further, these compounds were subjected to ADME property prediction, wherein 6 compounds were showing the parameter values within acceptable range. Toxicity prediction reveals the 5 compounds are non-toxic. Insilico investigations revealed that 5 phytoconstituents namely Gallic acid; 2,6-Dihydroxybenzoic acid, Kaempferol; N-(3-Benzooxazol-2-yl-4- hydroxy-phenyl)-2-ptolyloxyacetamide; and Glycophymoline of Cassia auriculata Linn methanol leaf extract are potentially inhibiting the inflammatory enzymes. The study identified the precise phytoconstituents from Cassia auriculata Linn for anti-inflammatory activity.

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