Phytotoxic, antioxidant and antifungal activity of crude methanolic extract of Equisetum debile

Paper Details

Research Paper 01/08/2013
Views (349) Download (8)

Phytotoxic, antioxidant and antifungal activity of crude methanolic extract of Equisetum debile

Muhammad Imran Khan, Mushtaq Ahmad, Rahmat Ali Khan, Amin Ullah, Syed Rehman, Bakhtar Ullah
Int. J. Biosci.3( 8), 130-135, August 2013.
Certificate: IJB 2013 [Generate Certificate]


Natural Products, especially plants, have been used for the treatment of various diseases for thousands of years. Terrestrial plants have been used as medicines in Egypt, China, India and Greece from ancient time and an impressive number of modern drugs have been developed from them. They are good and cheap sources of many useful bioactive chemical compounds which can be utilized as anitimirobial , anticancer and anti oxidant agents. In case of Equisetum debile , it have been used for the treatment of different ailments like infections, rheumatism and inflammation etc. The present study indicate that Equisetum debile methanolic extract (100 & 1000 µg/ml) significantly inhibit the growth of shoots (hypocotyls) and roots (radicals) of rice when compared to control after three and seven days treatment. Furthermore, the Equisetum debile methanolic extract (50 to 1000 μg/ml) exhibit antioxidant properties and scavenge the free radical in dose dependent manner when compared with standard antioxidant (ascorbic acid). Equisetum debile methanolic extract also have antifungal property that inhibit 42.26% and 53.84% growth of Aspergillus flavous and Aspergillus niger respectively, while using the extract 200 μg/ml.


Kokate CK(a), Purohit AP. “Text book of pharmacognosy” 2004, 29, p. 542.

Kokate CK(b), Purohit AP. “Text book of pharmacognosy”, 2004, 29, p.317-18, 336-37.7

Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E (2008). Antifungal, pytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technol 99, 8788-8795.

Kumar K, Nene YL. 1968. Anti-fungal properties of Cleome isocandra L. extracts. Indian Phytopath. 21, 445-449.

Loo AY, Jain K, Darah I (2007). Antioxidant and radical scavenging activities of pyroligeneous acid from a mangrove plant, Rhizophora apiculata. Journal of Food Chemistry 104, 300-307.

Molish H (1937). Der einfluss Ciner pflanze auf die andere- Allelopathic. The role of chemical inhibition in vegetation. Journal of Food Chemistery 1, 99-106.

Naczk M, Shahidi F. (2006). Phenolics in cereals fruits and vegetables: Occurrence, extraction, and analysis. Journal of Pharmaceutical and Biochemical Analysis.41, 1523–1542.

Nene YL, Thapliyal PN. 1965. Antifungal properties of Anagallis arvensis L. extracts. Nature 52, 89-90

Netzel M, NetzelG, Tian Q, Schwartz S, Konczak I. (2007). Native austraian foods–a novel source of antioxidants for food. Innovative Food Science and Emerging Technologies 8, 339–346.

Rice EL (1984) Allelopathy. Orlando/FL: Academic Press

Rice EL (1984). Allelopathy, 2nd edn. Academic press, New york.

Singh N. 2001. Trends in the epidemiology of opportunistic fungal infections: predisposing factors and the impact of antimicrobial use practices. Clinical Infect Diseases 33, (10), 1692-1696.

Steer P, Millgard J, Sarabi DM, Wessby B, Kahan T. (2002). Cardiac and vascular structure and function  are  related  to  lipid  peroxidation  and metabolism. Lipids 37, 231–236.

Valeria  FM,  Preve  L,  Tullio  V,  1996.  Fungi responsible for skin mycoses in Turin (Italy). Mycoses39, 141 150.

Sara  V,  Franca  T,  Gelsomina  F.  (2009). Traditional  uses  of  medicinal  Plants  in  Valvestino (Italy). Journal of Ethnopharmacol 121, 106–116.

Dubick AM. 1986. Historical perspectives on the use of Herbal preparations to promote health. Journal of Nutrition 116, 1348-1354.