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Plant and microbes interaction: the potential of Calotropis gigantea extract and latex towards bacterial strains

Research Paper | December 1, 2018

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Muhammad Numan, Iqbal Hussain, Ubaid Ahmad, Rahim Dost Khan, Mehmand Khan, Abdul Rehman, Warda Komal, Wali Muhammad, Syed Abdul Hadi

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Int. J. Biosci.13( 6), 95-101, December 2018

DOI: http://dx.doi.org/10.12692/ijb/13.6.95-101


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The microbial resistance has prominently diminished the efficacy of antibiotics and also the major cause of failure to treat bacterial infections. In the backdrop of this phenomenon, phytochemical or herbal medicines are gaining attention as the alternative source of natural antibiotics. In the present study, leaves extract and milk (latex) of Calotropis gigantea were tested for antibacterial activity. For this purpose, well diffusion method was evaluated fortwo-Gram positive (Staphylococcus aureus, Streptococcus pyogenes) and two-Gram negative (Escherichia coli, Klebsiella pneumonia) bacterial strains. Results revealed significant antimicrobial activities of the tested samples. The inhibition zones ofbacterial strains were observed in the range of 9 to 32 mm. At a concentration of 5 mg/ml leaves,activity was found good against S. pyogenes and K. pneumoniae while 16 days old milk (latex) against S. aureus. Excellent zones of inhibition were showed by latex against E. coli at a concentration of 10 mg/ml. E. coli and K. pneumoniae showed resistant to leaves extract and fresh milk (latex) at 15 mg/ml concentration. Leaves extract at a concentration of 20 mg/ml showed excellent inhibitory zones against S. aureus. Moreover, extract showed excellent inhibitory zones to S. pyogenes as compare to 5 days old milk (latex) at a concentration of 50 mg/ml. Furthermore, it was concluded that leaves extract and milk (latex) of C. gigantea could be used as an alternative to antibiotics against different diseases because of their substantial antibacterial activities.


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Plant and microbes interaction: the potential of Calotropis gigantea extract and latex towards bacterial strains

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