Pod resistance of cocoa clones to black pod disease and antifungal properties of phytoextracts against Phytophthora megakarya
Paper Details
Pod resistance of cocoa clones to black pod disease and antifungal properties of phytoextracts against Phytophthora megakarya
Abstract
In Cameroon, cocoa production is limited by constraints amongst which diseases such as black pod caused by Phytophthora megakarya which is the most aggressive in the country (80% losses). This study was aimed at determining the clonal resistance of six cocoa clones (IMC67, SCA12, TAFO79/501, TAFO79/467 ICS40, SNK13) both in field and Laboratory conditions, and to evaluate the antifungal activity of extracts from three plant species (Allium sativum, Zingiber officinale, Thymus algeriensis) and a product of the African traditional pharmacopoeia (African Panaxia) on the growth of Phytophthora megakarya. The pathogen was isolated on V8 medium and inoculated on pods. Disease incidence was evaluated in the cocoa plantation of the Mengang Production and Experimentation Center. Results show that, clones SCA12 and TAFO79/467 were the least susceptible to black pod disease in field and Lab conditions. African Panaxia, aqueous and ethanolic extracts of T. algeriensis induced total inhibition of P. megakarya at 62.50; 125.0 and 30.0 mg.ml-1, respectively. African Panaxia and ethanolic extracts of T. algeriensis had a fungicidal effect starting at 62.50 and 30.0 mg.ml-1, respectively while the aqueous extract of T. algeriensis had a fungistatic effect at 125.0 mg.ml-1. The study shows that clones SCA12 and TAFO79/467 are more tolerant to cocoa black pod. Moreover, due to their fungicidal effect against P. megakarya, African Panaxia and T. algeriensis extracts could be integrated into a management program against cocoa black pod disease.
André D, Barbara S. 2001. Practical guide to detection and identification of Phytophthora. Version 1.0, 42 p.
Bessedik ML. 2015. Etude de l’activité antifongique des huiles essentielles d’Eucalyptus globulus et Thymus algeriensis contre quelques champignons phytopathogènes des palmes du palmier dattier (Phoenix dactylifera L). Mémoire de Master académique, Université Kasdi Merbah Ouargla, Algérie, 93 p.
Ciftci M, Guler T, Simsek UG, Ertas ON, Dalkilic B, Bicer Z. 2009. growth promoter in broilers. Indian Veterinary Journal 86(9), 930-932.
Cilas C, Despréaux D. 2004. Improvement of Cocoa Tree Resistance to Phytophthora Diseases. Editions Quae, Technology and Engineering, 171 p.
Djeugap FJ, Akoula NC, Kyalo M, Njukeng AP, Galani YJH, Kuiate J-R, Ghimire S. 2017. Morphological and molecular identification of pathogenic fungi of Calabash nutmeg (Monodora myristica Dunal) kernels and their response to different phytoextracts. International Journal of Advanced Agricultural Research 5, 66 – 75.
Djocgoue PF. 1998. Analysis of variations in biochemical factors in development of infection caused by Phytophthora megakarya in Theobroma cacao L. Ph.D Thesis, University of Yaounde I, Yaounde, Cameroon.
Dohou N, Yamani K, Badoc A, Douira A. 2004. Activité antifongique d’extraits de Thymelaea lythroides sur trois champignons pathogènes du riz. Bull. Soc. Pharm. Bordeaux 143, 31-38.
Dooh PJ, Ambang Z, Bekolo N, Heu A, Tueguem KW. 2014. Effect of extracts of Thevetia peruviana (Pers.) K.Schum on development of Phytophthora megakarya causal agent of black pod disease of cocoa. Journal of Applied Biosciences 77, 6564 – 6574.
Dooh JP, Ambang Z, Ndongo B, Tueguem KW, Heu A, Ntsomboh-Ntsefong G. 2015. Development of cocoa black pod disease (caused by Phytophthora megakarya) in Cameroon when treated with extracts of Thevetia peruviana or Ridomil. International Journal of Current Research in Biosciences and Plant Biology 2(3), 47-59.
El-Wakeil NE. 2013. Botanical Pesticides and Their Mode of Action. Gesunde Pflanzen 65(4), 125–149.
Gallegly ME, Hong C. 2008. Phytophthora: Identifying Species by Morphology and DNA Fingerprints. American Phytopathological Society, 158 p.
Giweli AA, Džamić AM, Soković MD, Ristić MS, Marin PD. 2013. Central European Journal of Biology 8(5), 504–511.
Griffiths E. 1981. Iatrogenic plant diseases. Annual Review of Phytopathology 19, 69–82.
ICCO. 2013. International Cocoa Organization. The World Cocoa Economy: Past and present. London.
Imtiaj A, Rahman SA, Alam S, Parvin R, Farhana KM, Kim SB, Lee TS. 2005. Effect of fungicides and plant extracts on the conidial germination of Colletotrichum gloeosporioides causing mango anthracnose. Mycobiology 33(4), 200–205.
Kendrick B. 1981. Biology of Conidial Fungi. Cole G.T. Edition, Vol. 2, Academic Press, London, UK, 680 p.
Kim D-H. 2012. Chemical Diversity of Panax ginseng, Panax quinquifolium, and Panax notoginseng. J Ginseng Res. 36(1), 1–15.
Lee YS, Ju HK, Kim YJ, Lim TG, Uddin MR, Kim YB, Baek JH, Kwon SW, Lee KW, Seo HS, Park SU, Yang TJ. 2013. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation. PLoS One 8(12), e82479.
Losch B, Daviron B, Freud C, Gergely M. 1992. Relance régionalisée de la production paysanne de cacao et de café au Cameroun. Phase I. Cadrage général de la relance. MINAGRI/CIRAD/SOFRECO 2, 21 p.
Mahob RJ, Ndoumbè-Nkeng M, Ten Hoopen GM, Dibog L, Nyassé S, Rutherford M, Mbenoun M, Babin R, Amang J, Mbang A, Yede and Bilong Bilong CF. 2014. Pesticides use in cocoa sector in Cameroon: characterization of supply source, nature of actives ingredients, fashion and reasons for their utilization. International Journal of Biological and Chemical Science 8(5), 1976-1989.
MINADER (Mistere de l’Agriculture et du Developpement Rural). 2017. Government’s ban on Metalaxyl chemicals: farmers, reluctant to change. The Green Vision News Paper.
Mboussi SB, Ambang Z, Ndogho A, Dooh JPN, Manga EF. 2016. In vitro Antifungal Potential of Aqueous Seeds Extracts of Azadirachta indica and Thevetia peruviana against Phytophthora megakarya in Cameroon. Journal of Applied Life Sciences International 4(4), 1-12.
Mfegue CV. 2012. Origine et mécanismes de dispersion des populations de Phytophthora megakarya, pathogène du cacaoyer au Cameroun. PhD thesis, SupAgro, Montpellier, France, 185 p.
Ndoumbè-Nkeng M. 2002. Incidence des facteurs agro-écologiques sur l’épidémiologie de la pourriture brune des fruits du cacaoyer au Cameroun: contribution à la mise en place d’un modèle d’avertissements agricoles. Thèse de doctorat, Institut National Agronomique, France, Paris-Grignon, 151p.
Omokolo ND, Nankeu DJ, Niemenak N, Djocgoue PF. 2002. Analysis of amino acids and carbohydrates in the cortex of nine clones of Theobroma cacao L. in relation to their susceptibility to Phytophthora megakarya Bra. and Grif. Crop Protection 21, 395-402.
Raouf A, Khalil M. 2001. Phytofungitoxic properties in the aqueous extracts of some plants. Pakistan Journal of Biological Science 4(4), 392-394.
Serferbe S, Tsopmbeng NG, Yaouba A, Djeugap FJ, Keuete KE. 2015. Efficacy of three local plant extracts as seed treatment on the germination, infection and vigour index of two cotton seed varieties from Tchad. International Journal of Applied Biology and Pharmaceutical Technology 6(2), 39-44.
Slusarenko AJ, Patel A, Portz D. 2008. Control of plant diseases by natural products: Allicin from garlic as a case study. European Journal of Plant Pathology 121, 313-322.
Spotts RA, Cervantes LA. 1986. Population, pathogenicity and benomyl resistance of Botrytis spp., Penicillium spp., and Mucor piriformis in packinghouses. Plant Disease 70, 106–108.
Suleman MN, Emua SA. 2009. Efficacy of four plant extracts in the control of root rot disease of cowpea. African Journal of Biotechnology 8(16), 3806-3808.
Triki MA, Krichen W, Mallouli H, Samira K, Cheffi M, Aouissaoui H, Ikram J, Drira N, Hassaïri A. 2012. Activité antifongique de l’extrait d’ail vis-à-vis de quelques champignons isolés d’oliviers en dépérissement. Revue Ezzaitouna 13, 1-11.
Zhu J, Zhang Z, Yang Z. 2001. General research methods on pathogen of potato late blight (Phytophthora infestans). Journal of Agricultural Sciences 24, 112-114.
Joseph Djeugap Fovo, Villavienne Mezok Ntongo, Eric Bertrand Kouam, Fidèle Romuald Amougou Nsouga, Godswill Ntsomboh-Ntsefong (2018), Pod resistance of cocoa clones to black pod disease and antifungal properties of phytoextracts against Phytophthora megakarya; IJB, V13, N3, September, P94-104
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