Potential allelopathic inhibitory effect of Salix nigra on growth of Triticum aestivum

Paper Details

Research Paper 01/03/2016
Views (224) Download (4)

Potential allelopathic inhibitory effect of Salix nigra on growth of Triticum aestivum

Wasim Ahmed, Mushtaq Ahmad, Rahmat Ali Khan
Int. J. Biosci.8( 3), 29-34, March 2016.
Certificate: IJB 2016 [Generate Certificate]


The importance of the role of allelopathy in agro-eco system cannot be denied. Molisch, in 1937, put forwarded the concept of allelopathy to explain how a plant can impart its harmful/beneficial effects upon the other with the release of certain molecules in their surroundings. The present work was carried out as a preliminary study to investigate any possible herbicidal activity of the plant’s extract on growth of Triticum aestivum. Modified protocol of McLaughlin and Rogers was used to carry out petri plates study. Similarly, open field study was also carried out to get more precise results. Our results showed that the crude extract of our plant significantly (P > 0.05) inhibited the shoot (hypocotyls) growth of wheat compared to control after 3rd & 5th days. The data also indicated that the extract significantly inhibited roots growth as compared to non-treated water (control) group. After complete treatment with sample solutions, fresh and dry weight of all replicates was calculated and this exposed that our plant extract extensively (P < 0.05) controlled the fresh as well dry weight. Similarly, in field study, results indicated that it markedly (P<0.05) inhibited shoot growth compared to control. After complete treatment, fresh and dry weight of all replicates were measured which revealed that the extract significantly (P<0.05) decreased fresh as well dry weight of wheat.


Aziz M, Fujii Y. 2005. Allelopathic effect of some medicinal plant substances on germination of Amaranthus retroflexus and Portulaca. InProceeding, International Symposium on Improving the Performance of Supply Chains in the Transitional Economies. ISHS Acta Horticulturae 699.

Barkatullah M, Ibrar N, Muhammad. 2011. Evaluation of Zanthoxylum armatum DC for invitro and invivo pharmacological screening. African journal of pharmacy and pharmacology 5(14), 1718-1723. http://dx.doi.org/10.5897/AJPP11.405

Callaway RM, Aschehoug ET. 2000. Invasive plants versus their new and old neighbors: a mechanism for exotic invasion. Science 290, 521– 523.

Callaway RM. 2007. Positive interactions and interdependence in plant communities. Springer, Dordrecht.

Casper BB, Jackson RB. 1997. Plant competition underground. Annual Review of Ecological System. 28, 545–570.

Daniel P, John NK. 2006. Invasive plant suppresses the growth of native tree seedlings by disrupting belowground mutualisms. PLoS Biology. 4,727–731. http://dx.doi.org/10.1371/journal.pbio.0040140

Fujii Y, Hiradate S. 2007. Allelopathy : New concepts and methodology, Science publishers. 382 p.

Grime JP. 2001. Plant strategies, vegetation processes, and ecosystem properties. John Wiley & Sons, Chichester.

Huston M, Smith T. 1987. Plant succession: life history and competition. American Naturalist 130, 168–198.

Inderjit. 2011. Plant phenolics in allelopathy. Bot. Rev. 62, 186–202.

Jung EH, Lee IJ. 2009. Assessment of allelopathic potential of selected medicinal plants of Pakistan. African Journal of Biotechnology 8(6), 1024-1029.

Khan Al, Hamayun M, Hussain J, Khan H, Gillani SA, Kikuchi A, Watanab KN, Kristina AS, Stuart AC, Jeff RP, Benjamin EW, Ragan MC, Giles CT, Steven GH, Mangla S, Inderjit, Callaway RM. 2008. Exotic invasive plant accumulates native soil pathogens which inhibit native plants. Journal of Ecology. 96, 58–67.

McLaughlin JL, Rogers LL. 1998. The use of biological assays to evaluate botanicals. Drug Information Journal 32, 513-524.

Nazir T, Uniyal AK, Todaria NP. 2007. Allelopathic behaviour of three medicinal plant species on traditional agriculture crops of Garhwal Himalaya, India. Agroforestry systems 69(3), 183-7.

Prati D, Bossdorf O. 2004. Allelopathic inhibition of germination by Alliaria petiolata (Brassicaceae). American Journal of Botany. 91, 285–288.

Tilman D. 1988. Plant strategies and the dynamics and structure of plant communities. Princeton University Press, Princeton.

Wardle DA, Nilsson MC, Gallet C, Zackrisson 1998. An ecosystem‐level perspective of allelopathy. Biological Reviews. 73(3), 305-319. http://dx.doi.org/10.1111/j.1469185X.1998.tb00033.x

Wasim A, Mushtaq A, Nadia M, Abdul R, Zakir K. 2014. Phytotoxic and leishmanicidal activity of Salix nigra. International Journal of Biosciences. 5(7), 75-79. http://dx.doi.org/10.12692/ijb/5.7.75-79

Wasim A, Mushtaq A, Nadia M. 2014-2015. Phytochemistry and Anti-Acetyl Cholinesterase Activity of Salix nigra. International Journal of Pharmacognosy and Phytochemical Research 6(4), 942-944.