Potential of Clitoria ternatea L. flower extract as a safe and effective alternative to methylene blue stain
Paper Details
Potential of Clitoria ternatea L. flower extract as a safe and effective alternative to methylene blue stain
Abstract
The efficacy of Clitoria ternatea L. flower (Pukingan) aqueous extract for use in staining cheek cells was studied to obtain non-toxic, environmentally friendly and low-cost dyes for use in staining. C. ternatea L. has a sparsely pubescent stem that sub-erect and woody at the base and may be up to 5 m long. The flower extract of C. ternatea L. have anthocyanins which are categorized as ternatins. A number of histological techniques have been identified to be used to provide a nuclear stain consist of natural phenolic compounds, structurally related to anthocyanins. The objective of this study is to determine the efficacy of aqueous extract of Pukingan on cheek cells considering several parameters. Dye extracts from C. ternatea L. were used to stain cheek cells using the existing standard staining procedures with little modification. One Way Analysis of Variance was used to analyze differences among the mean scores. A significant difference was determined using a post hoc analysis which is Tukey’s test using SPSS and the level of significance was set at 0.05. From the results of the descriptive, parameters for all sample populations have almost similar interpretations. The nucleus was stained satisfactorily, and the sharpness and contrast were excellent. The cytoplasm of the cheek cells was stained intensely, and the stained areas are homogeneous. The prepared extracts had affinity for the cell membrane and nucleus. Therefore, this study shows that dye extracts from C. ternatea L. could be used for cheek cell staining as alternative to Methylene blue stain.
Chew Weng C, Abdullah R, Saad S. 2014. Alternative staining using extracts of hibiscus (Hibiscus rosa-sinensis L.) and red beet (Beta vulgaris L.) in diagnosing ova of intestinal nematodes (Trichuris trichiura and Ascaris lumbricoides) 1, 14-18
Cook BG, Pengelly BC, Brown SD, Donnelly JI, Eagles DA, Franco MA, Hanson J, Mullen BF, Partridge IJ, Peters M, Schultze-Kraft R. 2005. Tropical forages: an interactive tool [CD-ROM]. CSIRO, DPI & F (Qld), CIAT and ILRI, Brisbane, Australia
Eom S, Shin D, Yoon K, Indan J. 2001. Fibre Text 26(4), 425-421.
Mistry TV, Cai Y, Lilley TH, Haslam E. 1991. Polyphenol interactions. Part 5 Anthocyanin co-pigmentation. J Chem Soc, Perkin Trans 2(8), 1287-1296.
Nguyen D. 2017. What Is the Advantage of Using Stains to Look at Cells? Sciencing.com. Copyright 2017 Leaf Group Ltd. All Rights Reserved. Leaf Group Education
Onslow MW. 2013. The Anthocyanin Pigment of Plants. Google Books. Page 32. Retrieved from: www.books.google.com.ph/books
Staples IP. 1992. Clitoria ternatea L. In. T Mannetje L and Jones RM. Plant Resources of South-East Asia. 4. Forages Pudoc Scientific Publishers, Wagening. The Netherlands pp. 94-96
Vidana Gamage GC, Lim YY, Choo WS. 2021. Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications. Front. Plant Sci. 12, 792303. DOI: 10.3389/fpls.2021.792303
Webb MR, Min K, Ebeler SE. 2008. Anthocyanin Interactions with DNA: Intercalation, Topoisomerase I Inhibition and Oxidative Reactions. J Food Biochem 23, 32(5), 576-596. DOI: 10.1111/j.1745-4514.2008.00181.x. PMID: 19924259; PMCID: PMC2778027.
Ma Chrischelle F. Bullecer, 2023. Potential of Clitoria ternatea L. flower extract as a safe and effective alternative to methylene blue stain. Int. J. Biosci., 22(6), 165-170.
Copyright © 2023 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.