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Potential of Clitoria ternatea L. flower extract as a safe and effective alternative to methylene blue stain

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Research Paper 13/06/2023
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Potential of Clitoria ternatea L. flower extract as a safe and effective alternative to methylene blue stain

Ma Chrischelle F. Bullecer
Int. J. Biosci.22( 6), 165-170, June 2023.
Certificate: IJB 2023 [Generate Certificate]
Key: Alternative stainingClitoria ternateaMethylene blueNatural dye

Abstract

The efficacy of Clitoria ternatea L. flower (Pukingan) aqueous extract for use in staining cheek cells was studied to obtain non-toxic, environmentally friendly and low-cost dyes for use in staining. C. ternatea L. has a sparsely pubescent stem that sub-erect and woody at the base and may be up to 5 m long. The flower extract of C. ternatea L. have anthocyanins which are categorized as ternatins. A number of histological techniques have been identified to be used to provide a nuclear stain consist of natural phenolic compounds, structurally related to anthocyanins. The objective of this study is to determine the efficacy of aqueous extract of Pukingan on cheek cells considering several parameters. Dye extracts from C. ternatea L. were used to stain cheek cells using the existing standard staining procedures with little modification. One Way Analysis of Variance was used to analyze differences among the mean scores. A significant difference was determined using a post hoc analysis which is Tukey’s test using SPSS and the level of significance was set at 0.05. From the results of the descriptive, parameters for all sample populations have almost similar interpretations. The nucleus was stained satisfactorily, and the sharpness and contrast were excellent. The cytoplasm of the cheek cells was stained intensely, and the stained areas are homogeneous. The prepared extracts had affinity for the cell membrane and nucleus. Therefore, this study shows that dye extracts from C. ternatea L. could be used for cheek cell staining as alternative to Methylene blue stain.

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