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Potential of marine macroalgae against halomonas species isolated from the epithelial surface of infected adult Hippocampus kuda (yellow seahorse)

Research Paper | September 1, 2019

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Carl Nico Oñate, Sharon Rose Tabugo

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Int. J. Biosci.15( 3), 137-150, September 2019

DOI: http://dx.doi.org/10.12692/ijb/15.3.137-150


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An impediment to successful rearing of seahorses Hippocampus kuda (yellow seahorse) are microorganisms that serve as causative agent of diseases leading to high mortality and low survival rates in tanks. Antibiotics are used to treat such diseases but improper usage of antibiotics may promote resistant pathogens. The aim of this study was to isolate and identify bacteria species from infected seahorses with signs of ulcerative dermatitis through DNA barcoding via 16s rDNA bidirectional gene sequencing and determine marine algae with potential antibacterial activity in order to find alternate source for treatment. Results show three (3) strains of Halomonas species based on morphological characterization and DNA barcoding. Ethanol extraction was used to produce varying concentrations of algal extracts and were tested against the Halomonas species using Kirby-Bauer disc diffusion method. The zones of inhibition exhibited by the three different extracts against strains of Halomonas were not comparable to broad spectrum commercialized antibiotic Tetracycline (positive control) but showed great potential if percent concentration of extracts were to be increased. Thus, marine algae used in this study: Ulva intestinalis and Sargassum crassifolium can be a potential natural source of antibacterial compounds against pathogenic micoorganisms that pose a threat to seahorse aquaculture in general.


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Potential of marine macroalgae against halomonas species isolated from the epithelial surface of infected adult Hippocampus kuda (yellow seahorse)

Abdel-Khaliq A, Hassan HM, Rateb ME, Hammouda O. 2014. Antimicrobial activity of three Ulva species collected from some Egyptian Mediterranean seashores. International Journal of Engineering Research and General Science 2, 648-669.

Alcaide E, GilSanz C, Sanjuan E, Esteve D, Amaro C, Silveira L. 2001. Vibrio harveyi causes disease in seahorse, Hippocampus sp. Journal of Fish Diseases 24, 311-313. https://doi.org/10.1046/j.1365-2761.2001.00297x

Balcázar JL, Loureiro S, Da Silva YJ, Pintado J, Planas M. 2010. Identification and characterization of bacteria with antibacterial activities isolated from seahorses (Hippocampus guttulatus). The Journal of Antibiotics 63, 271-274. https://doi.org/10.1038/ja.2010.27

Baleta FN, Bolaños JM, Ruma OC, Baleta AN, Cairel JD. 2017. Phytochemicals screening and antimicrobial properties of Sargassum oligocystum and Sargassum crassifolium Extracts. Journal of Medicinal Plants 5, 382-387.

Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology 45, 493-496.

Binh DT, Quyen VDH, Sang TQ, Oanh TT. 2016. Vibriosis in Cultured Seahorse (Hippocampus spp.) in Khanh Hoa Province, Vietnam. International Journal of Innovative Studies in Aquatic Biology and Fisheries 2, 43-50.

Bruckner AW, Field JD, Daves N. 2005. The proceedings of the international workshop on Cites implementation for seahorse conservation and trade. NOAA Techn. Mem. NMFS-PR-36, Silver Spring, EE. UU.

Cartagena AB. 2014. Rearing of the seahorse Hippocampus guttulatus: Key factors involved in growth and survival (Doctoral dissertation, Universitat de les IllesBalears). http://hdl.handle.net/11201/270.6

Cavallo R, Acquaviva M, Stabili L, Cecere E, Petrocelli A, Narracci M. 2013. Antibacterial activity of marine macroalgae against fish pathogenic Vibrio species. Open Life Sciences 8, 646-653. https://doi.org/10.2478/s11535-013-0181-6

El-deen N. 2011. Screening for antibacterial activities in some marine algae from the red sea (Hurghada, Egypt). African Journal of Microbiology Research 5, 2160-2167. https://doi.org/10.5897/AJMR11.390

El Shafay SM, Ali SS, El-Sheekh MM. 2016. Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria. The Egyptian Journal of Aquatic Research 42, 65-74. https://doi.org/10.1016/j.ejar.2015.11.00.6

Garritty GM, Bell JA, Lilbum TG. 2004. Taxonomic Outline of The Prokaryotes Bergey’s Manual of Systematic Bacteriology, (2nd edn.), Springer, Newyork.

Garritty GM, Bell JA, Lilburn III T. 2005. Class III. Gammaproteobacteria class. Bergey’s Manual of Systematic Bacteriology 2 (Part B).

Huval JH, Latta R, Wallace R, Kushner DJ, Vreeland RH. 1995. Description of two new species of Halomonas: Halomonas israelensis sp. nov. and Halomonas canadensis sp. nov. Canadian journal of microbiology 41, 1124-1131. https://doi.org/10.1139/m95-156

Hudzicki J. 2009. Kirby-Bauer disk diffusion susceptibility test protocol.

Kim KK, Lee JS, Stevens DA. 2013. Microbiology and epidemiology of Halomonas species. Future microbiology 8, 1559-1573. https://doi.org/10.2217/fmb.13.108.

LePage V. 2012. A study of syngnathid diseases and investigation of ulcerative dermatitis (Doctoral dissertation). http://hdl.handle.net/10214/39.83

Liao WR, Lin JY, Shieh WY, Jeng WL, Huang R. 2003. Antibiotic activity of lectins from marine algae against marine vibrios. Journal of Industrial Microbiology and Biotechnology 30, 433-439. https://doi.org/10.1007/s10295-003-0068-7

Lin Q, Gao Y, Sheng J, Chen Q, Zhang B, Lu J.  2007. The effects of food and the sum of effective temperature on the embryonic development of the seahorse, Hippocampus kuda  Bleeker. Aquaculture 262, 481-492. https://doi.org/10.1016/j.aquaculture.2006.11011

Lourie SA, Foster SJ, Cooper EW, Vincent AC. 2004. A guide to the identification of seahorses. Washington DC, USA: Project Seahorse and TRAFFIC North America, 114.

Lustigman B, Brown C. 1991. Antibiotic production by marine algae isolated from the New York/New Jersey coast. Bulletin of Environmental Contamination and toxicology 46, 329-335. https://doi.org/10.1007/BF01688.928

Manilal A, Sujith S, Kiran GS, Selvin J, Shakir C, Gandhimathi R, Lipton AP. 2009. Antimicrobial potential and seasonality of red algae collected from the southwest coast of India tested against shrimp, human and phytopathogens. Annals of Microbiology 59, 207-219. https://doi.org/10.1007/BF03178.319

Manivannan K. 2011. Antimicrobial potential of selected brown seaweeds from Vedalai coastal waters, Gulf of Mannar. Asian Pacific journal of tropical biomedicine 1, 114. https://doi.org/10.1016/S2221-1691(11)60007-5

Michalak I, Chojnacka K. 2015. Algae as production systems of bioactive compounds. Engineering in Life Sciences 15, 160-176. https://doi.org/10.1002/elsc.2014001.91

Ncube NS, Afolayan AJ, Okoh AI. 2008. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 7, 1797-1806.

Olson ME, Ceri H, Morck DW, Buret AG, Read RR. 2002. Biofilm bacteria: formation and comparative susceptibility to antibiotics. Canadian Journal of Veterinary Research 66, 86.

 Raj ST, Lipton AP, Chauhan GS. 2010. Characterization and infectivity evaluation of Vibrio harveyi causing white patch disease among captive reared seahorses, Hippocampus kuda. Indian Journal of Geo-Marine Sciences 39, 151-156. http://hdl.handle.net/123456789/85.57

Regunathan C, Wesley SG. 2004. Control of Vibrio spp. in shrimp hatcheries using the green algae Tetraselmis suecica. Asian Fisheries Science 17, 147-158.

Rojas R, Miranda CD, Amaro AM. 2009. Pathogenicity of a highly exopolysaccharide-producing Halomonas strain causing epizootics in larval cultures of the Chilean scallop Argopecten purpuratus (Lamarck, 1819). Microbial ecology 57, 129. https://doi.org/10.1007/s00248-008-940.1-z

Salem WM, Galal H, Nasr El-deen F. 2011. Screening for antibacterial activities in some marine algae from the red sea (Hurghada, Egypt). African Journal of Microbiology Research 5, 2160-2167.

Sheng J, Lin Q, Chen Q, Gao Y, Shen L, Lu J. 2006. Effects of food, temperature and light intensity on the feeding behavior of three-spot juvenile seahorses, Hippocampus trimaculatus Leach. Aquaculture 256, 596-607. https://doi.org/10.1016/j.aquaculture.2006.02.02.6

Smit AJ. 2004. Medicinal and pharmaceutical uses of seaweed natural products: a review. Journal of applied phycology 16, 245-262. https://doi.org/10.1023/B:JAPH.0000047783.36600.ef

Vatsos IN, Rebours C. 2015. Seaweed extracts as antimicrobial agents in aquaculture. Journal of applied phycology 27, 2017-2035. https://doi.org/10.1007/s10811-014-05060

Vincent AC, Clifton-Hadley RS. 1989. Parasitic infection of the seahorse (Hippocampus erectus)—a case report. Journal of Wildlife Diseases 25, 404-406.

Yahya NA, Attan N, Wahab RA. 2018. An overview of cosmeceutically relevant plant extracts and strategies for extraction of plant-based bioactive compounds. Food and Bioproducts Processing 112, 69-85.