Welcome to International Network for Natural Sciences | INNSpub

Prediction of Tertiary Structure Homology between Bactericidal/Permeability Increasing Protein of Innate Immune System and Hydrolase Enzymes

Research Paper | July 15, 2014

| Download 1

Ashraf Gholizadeh

Key Words: Bactericidal, Permeability increasing protein, Hydrolase, Immune system, Esterase, Lipolytic

Int. J. Biosci.5( 2), 1-6, July 2014

DOI: http://dx.doi.org/10.12692/ijb/5.2.1-6


IJB 2014 [Generate Certificate]


Bactericidal/permeability increasing protein (BPI) is known as the important arm of the innate immune system that provides the first line of defense against various gram-negative bacterial infections in mammalians. The critical aspect of the antibacterial action of BPI against these bacteria is the binding of BPI to the envelope lipopolysaccharides (LPS). However, there is very rare information about the molecular events following the interaction between BPI and bacterial compounds. As the first ever time report, by using the available bioinformatics and computational tools, BPI protein family was predicted to be structurally and functionally comparable to lipolytic hydrolases particularly including esterase-lipases. This computational identification helped us to propose a potential mode of action for BPI family in mammalian immune system, opposing to the LPS hydrolysis by bacterial phospholipases.


Copyright © 2014
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Prediction of Tertiary Structure Homology between Bactericidal/Permeability Increasing Protein of Innate Immune System and Hydrolase Enzymes

Beamer LJ, Carroll SF, Eisenberg D. 1998. The BPI/LBP family of proteins: a structural analysis of conserved regions. Protein Science 7, 906-914.

Domingues MM, Castanho MARB, Santos NC. 2009. rBPI21 promotes lipopolysaccharide aggregation and exerts its antimicrobial effects by (hemi)fusion of PG-containing membranes. Plos ONE 4, 1-8. http://dx.doi.org/10.1371/journal.pone.0008385

Elsbach P, Weiss J. 1998. Role of bactericidal permeability increasing protein in host defense. Current Opinion in Immunology 10, 45-49. http://dx.doi.org/10.1016/S0952-7915(98)80030-7

Elsbach P. 1998. The bactericidal/permeability-increasing protein (BPI) in antibacterial host defense. Journal of Leukocyte Biology 64, 14-18.

Gonzalez MY, Destoumieux-Garzon GD, Romestand B, Fievet J, Pugniere M, Roquet F, Escoubas JM, Vandenbulcke F, Levy O. 2007. Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas. Proceedings of the National Academic of Sciences USA 104, 17759-17764.

Gray PWG, Flaggs G, Leong SR, Gumina RJ, Weiss J, Ooi CE, Elsbach P. 1989. Cloning of the cDNA of a human neutrophil bactericidal protein: structural and functional correlations. Journal of Biological Chemistry 264, 9505-9509.

Horwitz AH, Carroll SF, Williams RE, Liu PS. 2000. Inclusion of S-sepharose beads in the culture medium significantly improves recovery of secreted rBPI21 from transfected CHO-K1 cells. Protein Expression and Purification 18, 77-85. http://dx.doi.org/10.1006/prep.1999.1163

Inagawa HT, Kohchi HC, Nishizawa T, Yoshiura Y, Nakanishi T, Yokomizo Y, Soma G. 2002. Cloning and characterization of the homologue of mammalian lipopolysaccharide binding protein and bactericidal permeability increasing protein in rainbow trout Oncorhynchus mykiss. Journal of Immunology 168, 5638-5644.

Iovine N, Eastvold J, Elsbach P, Weiss JP, Gioannini TL. 2002. The carboxyl-terminal domain of closely related endotoxin-binding proteins determines the target of protein-lipopolysaccharide complexes. Journal of Biological Chemistry 277, 7970-7978. http://dx.doi.org/10.1074/jbc.M109622200

Lennartsson A, Pieters K, Vidovic K, Gullberg U. 2005. A murine antibacterial ortholog to human bactericidal permeability increasing protein (BPI) is expressed in testis, epididymis, and bone marrow. Journal of Leukocyte Biology 77, 369-377. http://dx.doi.org/10.1189/jlb.0304159

Reddy KVR, Yedery RD, Aranha C. 2004. Antimicrobial peptides: premises and promises. International Journal of Antimicrobial Agents 24, 536-547. http://dx.doi.org/10.1016/j.ijantimicag.2004.09.005

Takahashi M, Horiuchi Y, Tezuka T. 2004. Presence of bactericidal permeability increasing protein in human and rat skin. Experimental Dermatology 13, 55-60. http://dx.doi.org/10.1111/j.0906-6705.2004.00116.x

Weiss J, Victor M, Cross AS, Elsbach P. 1982. Sensitivity of K1-encapsulated Escherichia coli to killing by the bactericidal permeability increasing protein of rabbit and human neutrophils. Infection and Immunity 38, 1149-1153.