Preliminary phyto-chemical screening and comparative antioxidant potential of Alstonia scholaris leaves extracts
Paper Details
Preliminary phyto-chemical screening and comparative antioxidant potential of Alstonia scholaris leaves extracts
Abstract
Yet to now natural products, or its’ derivatives are in service to humankind as the best source of molecules to control the pest and disease problems of human beings. The present work investigated total phenolic content, total flavonoid content, reducing power capacity, total antioxidant capacity, DPPH free radial scavenging activity of four different extracts of the plant A. scholaris which belongs to the family Apocynaceae. Total phenolic content (21.92± 0.13 mg GAE/g) and total flavonoid content (16.61± 0.06 mg CatE/g) were found to be highest in Dia-ion resin adsorbed fraction, and the same fraction showed the highest total antioxidant activity with absorbance 1.049±0.014 at 100 mg/mL as well as highest DPPH radical scavenging activity with IC50 value 24.90 mg/mL. The Iron reducing power of the different extractives and standard exhibited the following order: Ascorbic acid> DRAF > CLF > EAF > PEF and the total antioxidant activity of different extractives and standard exhibited the following order: Ascorbic acid> DRAF > PEF > EAF > CLF.
Akinpelu DA, Onakoya TM. 2006. Antimicrobial activities of medicinal plants used in folklore remedies in south-western. African journal of Biotechnology 5(11).
Antony M, Menon DB, Joel J, Lipin D, Arun K, Thankamani V. 2011. Phytochemical analysis and antioxidant activity of Alstonia scholaris. Pharmacognosy Journal 3(26), 13-8.
Asolkaret LV, Kakkar KK, Chakra OJ. 1992. Second supplement to glossary of Indian medicinal plants with active principles, part 1(A-K).XIVII+414, Pub. And Inf. Div. (CSIR), New Delhi.
Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. 2001. Antioxidant principles from Bauhinia terapotensis. Journal of Natural Product 64, 892–895.
Butler MS. 2004. The role of natural product chemistry in drug discovery. Journal of Natural Product, 67, 2141–2153.
Cherian S, Augusti KT. 1995. Insulin sparing action of leucopelargonin derivative isolated from Ficus bengalensis Linn. Indian Journal of Experimental Biology 33, 608-11.
Dahlia IA, Islam MB, Zaman S, Islam MA, Jalil MA, Ahmed NU, Rahim MA, Mondol MM, Muzahid AA, Sarkar MH. 2017. In-vitro DPPH Free Radical Scavenging Activity of the Plant Murraya koenigii Linn (Curry Leaf) in Rajshahi, Bangladesh. Journal of Complementary and Alternative Medical Research 15, 1-9.
Farnsworth NR. 1988. Sereening plants for new medicines. Wilson EO(Ed.). Biodiversity, pp.83-97, National Academy Press, Washington.
Harborne JB. 1973. Phytochemical methods. Chapman and hall Ltd., London, UK. 49-188.
Ikram M, Inamul H. 1984. Screening of medicinal plants for antimicrobial activities, Fitoterapia 55, 62-64.
Karthishwaran K, Mirunalini S, Dhamodharan G, Arulmozhi V. 2010. Phytochemical investigation of methanolic extract of the leaves of Pergularia daemia. Journal of biological sciences 10(3), 242-246.
Kaushik P, Kaushik D, Sharma N, Rana AC. 2011. Alstonia scholaris: It’s Phytochemistry and pharmacology. Chronicles of Young Scientists 2, 71-78.
Khan AV, Parveen G, Alam MM, Singh VK. 2002. Ethnomedicinal uses of Neem in rural areas of Uttar Pradesh, India. Ethnomed and Pharmacog. II Rec. Prog. Med. Plants, p7.
Middleton E, Jr. 1996. Biological properties of plant flavonoids: an overview. Int Pharmacognosy, 34, 344-348.
Misra CS, Pratyush K, Sagadevan LD, James J, Veettil AK, Thankamani V. 2011. A comparative study on phytochemical screening and antibacterial activity of roots of Alstonia scholaris with the roots, leaves and stem bark. International Journal of Research in Phytochemistry and Pharmacology, 1(2), 77-82.
Ogawa S, Kimura H, Niimi A, Katsube T, Jisaka M, Yokota K. 2008. Fractionation and structural characterization of polyphenolic antioxidants from seed shells of Japanese horse chestnut (Aesculus turbinata BLUME). Journal of Agricultural and Food Chemistry 56(24), 12046-51.
Oyaizu M. 1986. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition 44, 307-315.
Perry LM, Metzer J. 1998. Medicinal plants of east and south Asia, attributed properties and uses. Massachisetts and London: MIT press, Cambridge.
Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E. Analytical Biochemistry 269, 337-341.
Rastogi R, Mehrotra BN. 1991. Compendium of Indian Medicinal Plants, Vol. III 1: 497, CSIR Publication, India.
Ray PG, Majumdar SK. 1976. Antimicrobial activity of some Indian Plants. Economic Botany, 30(4), 317-329.
Reddy DS. 2016. Phytochemical Analysis of Active Constituents of Alstonia scholaris and their Cytotoxicity in vitro. International Journal of Pharmaceutical Sciences and Research 7(8), 3262.
Shah AJ, Gowani SA, Zuberi AJ, Ghayur MN, Gill AH. 2010. Antidiarrhoeal and Spasmolytic Activities of the methanolic Crude Extract of Alstonia scholaris L. are mediated through Calcium Channel Blockade. Phytotherapy Research 24, 28-32.
Shang JH, Cai XH, Feng T, Zhao YL, Wang JK, Zhang LY, Yan M, Luo XD. 2010. Pharmacological evaluation of Alstonia scholaris: Anti-inflammatory and analgesic effects. Journal of Ethnopharmacology 29(2), 174-81.
Singh SK, Singh A. 2003. Molluscicidial and Anti-cholinerstase Activity of Alstonia scholaris plant against Freshwater Snail Lymnaea acuminate. Pakistan Journal of Biological Science 6(16), 1442-1446.
Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture 16(3), 144-58.
Sofowara A. 1993. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria 289-300.
Soto ML, Conde E, González-López N, Conde MJ, Moure A, Sineiro J, Falqué E, Domínguez H, Núñez MJ, Parajó JC. 2012. Recovery and Concentration of Antioxidants from Winery Wastes. Molecules 17(3), 3008-3024.
Stauth D. 2007. Studies Force New View on Biology of Flavanoids. Oregon State University, USA.
Teixeira RO, Camparoto ML, Mantovani MS. 2003. Assessment of two medicinal plants Psidium guajava L. and Achilleamille folium L. In vitro and in vivo assays. Genetics and Molecular Biology 26(4), 551-555.
Yamauchi T, Abe F, Padolina WG, Dayrit FM. 1990. Alkaloids from leaves and bark of Alstonia scholaris in the Philippines. Phytochemistry, 29(10), 3321-3325.
Yoshida T, Mori K, Hatano T, Okomura T, Uehara I, Komagoe K, Fujita Y, Okuda T. 1989. Studies on inhibition mechanism of autoxidation by tannins and flavonoids. Radical-scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrlhydrazyl radical. Chemical and Pharmaceutical Bulletin 37(7), 1919-1921.
Zhao S, Liu JY, Chen SY, Shi LL, Liu YJ, Ma C. 2011. Antioxidant Potential of Polyphenols and Tannins from Burs of Castaneamollissima Blume. Molecules 16, 8590-8600.
Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64, 555-559.
Md. Tohidur Rahman, Md. Asadul Islam, Md. Abdul Jalil, Nazim Uddin Ahmed, Md. Abdurrahim, Md. Mahmudul Hassan Mondol, Ali Ahsan Muzahid, Md. Badrul Islam, ABM Hamidul Haque, 2019. Preliminary phyto-chemical screening and comparative antioxidant potential of Alstonia scholaris leaves extracts. Int. J. Biosci., 15(4), 161-171.
Copyright © 2019 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.