Preliminary yield and growth performance of rice (var nsic rc222) applied with Bacillus spp. based bio-fertilizer

Paper Details

Research Paper 06/03/2026
Views (10)
current_issue_feature_image
publication_file

Preliminary yield and growth performance of rice (var nsic rc222) applied with Bacillus spp. based bio-fertilizer

Ronneil B. Alminar*, Analyn V. Sagun, Angelina T. Gonzales
J. Biodiv. & Environ. Sci. 28(3), 39-48, March 2026.
Copyright Statement: Copyright 2026; The Author(s).
License: CC BY-NC 4.0

Abstract

This study aimed to find out and document the performance of NSIC Rc 222 rice variety as influence by application of Bio-fertilizer.  Particularly, it aimed to find out the determine the growth and yield performance of the rice applied with  bio-fertilizer at 10 days after first application,  and to determine the profitability of NSIC Rc 222 rice variety . The study was conducted from July to December 2025 in Barangay Antonino, Balaoan, La Union to evaluate the performance and profitability of NSIC Rc 222 rice applied with a Bacillus-based biofertilizer at 10 days after transplanting (DAT). Using a Randomized Complete Block Design (RCBD) with three replications and following the PalayCheck system, standard soil analysis, fertilizer recommendations, crop establishment, irrigation, and integrated pest management practices were implemented to ensure reliable results. Biofertilizer application at 10 DAT significantly increased panicle length and the number of filled grains, indicating improved grain filling and overall yield performance. Although reductions in unfilled grains and slight differences in 1000-grain weight were observed, these were not statistically significant. The treatment also enhanced vegetative growth and tiller productivity, suggesting that early application supports better crop establishment and development. Economically, despite higher production costs, the 10 DAT treatments generated higher gross and net income, resulting in the highest return on investment among the treatments. Early application of Bacillus-based biofertilizer at 10 DAT proved to be the most agronomically effective and financially viable strategy for improving the productivity and profitability of NSIC Rc 222 rice.

Abao DC, Aquino JC, Guzman AC de, Francisco AM, Rafa AA, Cruz JB. 2022. Philippine farmers adaptation of hybrid rice technology to increase profitability and contribute to rice sufficiency. International Journal of Advanced Engineering, Management and Science 8(3).

Ammar EE, Aioub AAA, Elesawy AE, Karkour AM, Mouhamed MS, Amer AA, EL-Shershaby NA. 2022. Algae as bio-fertilizers: between current situation and future prospective: the role of algae as a bio-fertilizer in serving of ecosystem. Saudi Journal of Biological Sciences 29(5). https://doi.org/10.1016/j.sjbs.2022.03.020

Author C. 2022. Effect of different nitrogen levels and bio-fertilizers along with banana pseudostem sap on summer hybrid rice (Oryza sativa L.) under south Gujarat condition. The Pharma Innovation Journal 11(3), 485-490.

Bai Y, Chang Y, Hussain M, Lu B, Zhang J, Song X, Lei X, Pei D. 2020. Soil chemical and microbiological properties are changed by long-term chemical fertilizers that limit ecosystem functioning. Microorganisms 8(5), 694.

Chen Y, Li X, Tian H, Zhang J, Liu X. 2020. Genetic regulation of grain weight and its response to nutrient management in rice (Oryza sativa L.). Field Crops Research 250, 107763. https://doi.org/10.1016/j.fcr.2020.107763

Dait JMG. 2023. A panel data study on factors affecting rice production in the Philippines.

Department of Agriculture. 2018. The Philippine rice industry roadmap 2030: Toward rice secure Philippines.

Fahmid I, Jamil A, Wahyudi, Agustian A, Hatta M, Aldillah R, Yofa R, Sumedi, Sumaryanto, Susilowati S. 2022. Study of the impact of increasing the highest retail price of subsidized fertilizer on rice production in Indonesia. Open Agriculture 7(1), 348–359. https://doi.org/10.1515/opag-2022-0087

Fukagawa NK, Ziska LH. 2019. Rice: importance for global nutrition. Journal of Nutritional Science and Vitaminology 65(Supplement), S2–S3.

Gao C, El-Sawah AM, Ismail Ali DF, Hamoud YA, Shaghaleh H, Sheteiwy MS. 2020. The integration of bio and organic fertilizers improve plant growth, grain yield, quality and metabolism of hybrid maize (Zea mays L.). Agronomy 10(3). https://doi.org/10.3390/agronomy10030319

Gu Y, Wang X, Yang T, Friman VP, Geisen S, Wei Z, Shen Q. 2020. Chemical structure predicts the effect of plant-derived low-molecular weight compounds on soil microbiome structure and pathogen suppression. Functional Ecology 34(10), 2158–2169.

Hashem A, Tabassum B, Abd Allah EF. 2019. Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences 26(6), 1291–1297. https://doi.org/10.1016/j.sjbs.2019.05.004

International Rice Research Institute (IRRI). 2013. Standard evaluation system for rice (5th Ed.). International Rice Research Institute.

Jane-Atilano-Sabalberino A. 2023. Forecasting value of production of palay and retail price of rice in the Philippines using ARIMA modelling. World Journal of Advanced Research and Reviews 17(3), 035–054.

Jin N, Jin L, Wang S, Li J, Liu F, Liu Z, Luo S, Wu Y, Lyu J, Yu J. 2022. Reduced chemical fertilizer combined with bio-organic fertilizer affects the soil microbial community and yield and quality of lettuce. Frontiers in Microbiology 13. https://doi.org/10.3389/fmicb.2022.863325

Kartika K, Lakitan B, Kadir S, Wijaya A, Siaga E, Widuri LI, Lindiana L, Meihana M. 2023. Timing of fertilizer applications and rice cropping systems at riparian wetland in South Sumatra, Indonesia. AIP Conference Proceedings 2972(1). https://doi.org/10.1063/5.0182831

Kumar GN, Singh PK, Naresh RK. 2024. Influence of planting techniques and integrated nutrient management on soil health in rice (Oryza sativa L.). International Journal of Environment and Climate Change 14(1). https://doi.org/10.9734/ijecc/2024/v14i13859

Kumawat KC, Kumari N. 2024. Biofertilizer’s application strategies for agricultural sustainability development. Indian Farming 74(6), 10–14.

Liu Q, Pang Z, Yang Z, Nyumah F, Hu C, Lin W, Yuan Z. 2022. Bio-fertilizer affects structural dynamics, function, and network patterns of the sugarcane rhizospheric microbiota. Microbial Ecology 84(4). https://doi.org/10.1007/s00248-021-01932-3

Lojo K. 2021. IRRI news: Philippine rice farmers receive 46k bags of two IRRI-developed inbreds from government program.

Lu H, Qi X, Li P, Qiao D, ur Rahman S, Bai F, Cui J. 2022. Effects of Bacillus subtilis and Saccharomyces cerevisiae inoculation on soil bacterial community and rice yield under combined irrigation with reclaimed and fresh water. International Journal of Agricultural and Biological Engineering 15(3), 33–46.

Mosisa W, Dechassa N, Kibret K, Zeleke H, Bekeko Z. 2022. Effects of timing and nitrogen fertilizer application rates on maize yield components and yield in eastern Ethiopia. Agrosystems, Geosciences and Environment 5(4). https://doi.org/10.1002/agg2.20322

Parthasarathi R, Elango R, Srimannarayanan J, Akash K, Kavinilavu N. 2021. Effects of bio-fertilizer on growth and yield of rice (Oryza sativa) AU1GSR cultivar. International Journal of Botany Studies 6(5), 673–676.

Patil S, Diwan JR, Mahantashivayogayya K, Swamy M, Pramesh D. 2025. Effect of Bacillus subtilis on rice seedling growth in Hoagland’s nutrient solution: A study in plant-microbe interactions. International Journal of Advanced Biochemistry Research 9(Special Issue 9), 841–843. https://doi.org/10.33545/26174693.2025.v9.i9Sk.5608

Patriyawaty NR, Agustina K. 2022. Growth and yield response of lowland rice to form and dosage of bio-fertilizer at different plant spacing. IOP Conference Series: Earth and Environmental Science 995(1), 012008.

Pratiwi GR, Nurrahma AHI, Pratiwi E, Yuniarti E, Ikhwani I. 2023. The effect of biofertilizer on growth and yield of lowland rice at alluvial soil. Agrosains: Jurnal Penelitian Agronomi 25(2), 56–63.

Rahman MM, Islam MS, Uddin S, Hasan MM. 2021. Panicle development and yield of rice as affected by bio-fertilizer and nitrogen management. International Journal of Agronomy 2021, 8894213. https://doi.org/10.1155/2021/8894213

Ren X, Chen F, Ma T, Hu Y. 2020. Soil quality characteristics as affected by continuous rice cultivation and changes in cropping systems in South China. Agriculture 10(10), 443.

Shrestha J, Kandel M, Subedi S, Shah KK. 2020. Role of nutrients in rice (Oryza sativa L.): a review. Agrica 9(1), 53–62.

Singh R, Verma P, Singh S, Yadav RK. 2021. Timing and frequency of bio-fertilizer application influence vegetative growth and nutrient uptake in rice. Indian Journal of Agronomy 66(4), 456–462.

Suleiman AKA, Lourenço KS, Clark C, Luz RL, da Silva GHR, Vet LE, Kuramae EE. 2020. From toilet to agriculture: Fertilization with microalgal biomass from wastewater impacts the soil and rhizosphere active microbiomes, greenhouse gas emissions and plant growth. Resources, Conservation and Recycling 161, 104924.

Sun Y, Xing M, He Z, Sun Y, Deng Y, Luo Y, Ma J. 2024. Effects of urea topdressing time on yield, nitrogen utilization, and quality of mechanical direct-seeding hybrid indica rice under slow-mixed fertilizer base application. Frontiers in Plant Science 15, 1400146.

Sunarpi H, Nikmatullah A, Sunarwidhi A, Sapitri I, Ilhami BK, Widyastuti S. 2020. Growth and yield of rice plants (Oryza sativa) grown in soil media containing several doses of inorganic fertilizers and sprayed with Lombok brown algae extracts. IOP Conference Series: Earth and Environmental Science 594.

Tiwari SK. 2023. Preparation of bio-fertilizer by using kitchen waste and biomass and effect of their application on crops. Pollution Research 42(1). https://doi.org/10.53550/pr.2023.v42i01.004

Ye L, Zhao X, Bao E, Li J, Zou Z, Cao K. 2020. Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality. Scientific Reports 10(1). https://doi.org/10.1038/s41598-019-56954-2

Yu B. 2023. Study on the effects of different fertilization times on crop yield and soil fertility changes. E3S Web of Conferences 416. https://doi.org/10.1051/e3sconf/202341601009

Zhang K, Khan Z, Khan MN, Luo T, Luo L, Bi J, Hu L. 2024. The application of biochar improves the nutrient supply efficiency of organic fertilizer, sustains soil quality and promotes sustainable crop production. Food and Energy Security 13(1), e520.

Zhou Y, Gao S, Wei J, Chen X, Zhu S, Zhou X. 2023. Systematical construction of rice flavor types based on HS-SPME-GC–MS and sensory evaluation. Food Chemistry 413, 135604.

Zhu F, Wang J, Jia Y, Tian C, Zhao D, Wu X, Li Y. 2021. Bacillus subtilis GB519 promotes rice growth and reduces the damages caused by rice blast fungus Magnaporthe oryzae. PhytoFrontiers 1(4), 330–338.

Zhu H, Zhou H, Ren Z, Liu E. 2022. Control of Magnaporthe oryzae and rice growth promotion by Bacillus subtilis JN005. Journal of Plant Growth Regulation 41(6), 2319–232.

Related Articles

Preliminary floral and faunal species diversity in Maluyo River in Santol, La Union, Philippines

Judith M. Morales*, Analyn V. Sagun, Angelina T. Gonzales, J. Biodiv. & Environ. Sci. 28(4), 26-39, April 2026.

Challenges and impact of the farmer-scientists training program on community development in Bohol, Philippines

Jeffrey O. Awas*, Anabel J. Intong, Aida T. Salingay, Manolito C. Macalolot, J. Biodiv. & Environ. Sci. 28(4), 8-25, April 2026.

Morphometric and biochemical responses of rice seedlings to heavy metal stress mitigated by Bacillus subtilis

J. Sujatha, V. Vinotha, R. Rajakumar*, J. Biodiv. & Environ. Sci. 28(3), 28-38, March 2026.

The mangrove of Tablas Island, Romblon, Philippines: Composition, conservation status, distribution and threats

Jeric B Gonzalez*, Xyrra Jeremiah C. Mazo-Gonzalez, John Benedic M. Natata, J. Biodiv. & Environ. Sci. 28(3), 8-27, March 2026.

Microplastics in Indian squid (Uroteuthis duvaucelii ) in region 1, Philippines

John Dave B. Orpilla*, J. Biodiv. & Environ. Sci. 28(3), 1-7, March 2026.

Using chitosan made from modified chitosan (Crab shells) for dye adsorption, equilibrium, kinetic, and response surface methods

M. Priyanga, V. Gomathi Priya, P. Bhuvaneswari, T. Shanmuga Vadivu, S. Viswanathan, G. Annadurai, R. Soranam*, J. Biodiv. & Environ. Sci. 28(2), 85-98, February 2026.

Effects of logging regimes on woody species diversity and stand structure in community forests adjacent to the Dja biosphere reserve, Cameroon

Nanga Charnelle Prudence*, Angoni Hyacinthe, Menyene Etoundi Laurent Florent, Ifo Averti Suspense, Nkemnkeng Francoline Jong, Mbolo Marie Marguerite, J. Biodiv. & Environ. Sci. 28(2), 76-84, February 2026.