Presence of seventeen genes potentially involved in cold tolerance in sugarcane and Saccharum spontaneum genotypes

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Research Paper 01/01/2019
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Presence of seventeen genes potentially involved in cold tolerance in sugarcane and Saccharum spontaneum genotypes

Shafee Ur Rehman, Khushi Muhammad, youxiong que, Atta Ur Rehman, Evandro Novaes, Sajjad Khan
Int. J. Biosci.14( 1), 346-355, January 2019.
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Cold is one of the most important stress which effect the growth and productivity of plants. Various pathways and mechanisms are affected by cold stress in plants. In the present work the Genomic DNA of three sugarcane genotypes and another from the wild species Saccharum spontaneum were tested for the presence of 17 candidate’s genes/miRNA involved in cold stress tolerance. Among these genotypes two sugarcane cultivars are cold tolerant, namely CP 85_1491 and SPSG 394, while the CP 77_400 is cold susceptible. Presence of these 17 gene/miRNA was confirmed by PCR and gel electrophoresis. In the genomic DNA of cultivar SPSG 394 all genes were amplified while the CP 85_1491 and S. spontaneum showed amplification for 96% of the genes/miRNAs and low results of these genes were studied in Genotype CP77_400. Therefore we concluded that cultivar SPSG 394 are more cold tolerant.


Bolle C. 2004. The role of GRAS proteins in plant signal transduction and development. Planta 218, 683–692.

Curaba J, Talbot M, Li ZY, Helliwell C. 2013. Over-expression of microRNA171 affects phase transitions and floral meristem determinancy in barley. BMC Plant Biology 13, 6.

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13-15.

Du YC, Nose A, Wasano K. 1999. Thermal characteristics of C4 photosynthetic enzymes from leaves of three sugarcane species differing in cold sensitivity. Plant Cell Physiology 40, 298–304.

Engstrom EM, Andersen CM, Gumulak-Smith J,  Hu J, Orlova E, Sozzani R. 2011. Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy. Plant Physiology 155(73), 5–750.

Janská A, Hodek J, Svoboda P, Zámˇcník J, Prášil IT,  Vlasáková E. 2013. The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress. Molecular Genetics Genomics 288, 639–649.

Lam E, Shine J, DaSilva J, Lawton M, Bonos S, Calvino M, Carrer H,  SilvaFilho MC, Glynn N, Helsel Z. 2009. Improving sugarcane for biofuel: engineering for an even better feedstock. Global change biology Bioenergy 1(3), 251–255.

Lee MH, Kim B, Song SK, Heo JO, Yu NI, Lee SA. 2008. Largescale analysis of the GRAS gene family in Arabidopsis thaliana. Plant Molecular Biology 67, 659–670.

Lynch DV. 1990. Chilling injury in plants: the relevance of membrane lipids. In: F. Katterman, Environmental Injury to Plants. Academic. Press. New York 17, 34.

Mikkelsen MD, Naur P, Halkier BA. 2004. Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3acetaldoxime in auxin homeostasis. Plant Journal 37, 770-777.

Moore PH. 1987. Breeding for stress resistance. In. Heinz. D. J. ed. Sugarcane Improvement through Breeding 503, 542.

Nogueira FTS, De Rosa Jr VE, Menossi M, Ulian EC, Arruda P. 2003. RNA expression profiles and data mining of sugarcane response to low temperature. Plant Physiology 132, 1811–1824.

Pearce RS. 1999. Molecular analysis of acclimation to cold. Plant Growth Regulation 29, 47–76.

Que Y, Su Y, Guo J, Wu Q, Xu L. 2014. A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNAseq. PLoS ONE. Journal pone 9(8), 106476.

Sakakibara H. 2006. Cytokinins: activity, biosynthesis, and translocation. Annual Review Plant Biology, 57, 431-449.

Tai PYP, Lentini RS. 1998. Freeze damage of Florida sugarcane. In D.L Anderson. Sugarcane Handbook, Ed 1. Florida. Cooperative. Extension. Service. University of Florida, Gainesville. FL. 1–3.

Thiebaut F, Rojas CA, Almeida KL, Grativol C, Domiciano GC, Lamb CR. 2012. Regulation of miR319 during cold stress in sugarcane. Plant Cell Environment 35, 502–512.

Thomashow MF.  2001. So what’s new in the field of plant cold acclimation? Lots. Plant Physiology 125, 89–93.

Uemura M, Joseph RA, Steponkus PL. 1995. Cold acclimation of Arabidopsis thaliana Effect on plasma membrane lipid composition and freeze-induced lesions. Plant Physiology 109, 15-30.

Vandesompele J, De-Preter K, Pattyn F, Poppe B, Van Roy N, De-Paepe A. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3, 0034.

Wang B, Sun YF, Song N, Wei JP, Wang XJ, Feng H. 2014. MicroRNAs involving in cold, wounding and salt stresses in Triticum aestivum L. Plant Physiology. Biochemistry 80, 90–96.

Xia J, Zhao H, Liu W, Li L, He Y. 2009. Role of cytokinin and salicylic acid in plant growth at low temperatures.  Plant Growth Regulation 57, 211-221.

Xin Z, Browse J. 2000. Cold comfort farm the acclimation of plants to freezing temperatures. Plant Cell Environment 23, 893-902.

Zhang J, Xu Y, Huan YY, Chong QK. 2009. Deep sequencing of Brachypodium small RNAs at the global genome level identifies microRNAs involved in cold stress response. BMC Genomics 10, 449.