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Preservation of epididymal spermatozoa of garut ram in soybean lechitin-based extender

Research Paper | July 1, 2017

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Muhammad Rizal, Muhammad Thahir, Yulnawati Yusnizar

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Int. J. Biosci.11( 1), 232-239, July 2017

DOI: http://dx.doi.org/10.12692/ijb/11.1.232-239


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Objective of the research was to obtain the best ratio between Andomed® and bidestilled water in maintaining quality of garut ram epididymal spermatozoa, preserved at 5 oC. Spermatozoa was collected by the combination of slicing, flushing, and pressure of cauda epididymis tissue using physiological saline (0.9% NaCl). The collected-spermatozoa was divided in equal volume into four tubes and centrifuged at 3,000 rpm for 20 min. Spermatozoa pellet in each tubes were diluted up to 200.106 cells/ml with Tris-egg yolk (control extender), 15% of Andromed® (Andro15), 20% of Andromed® (Andro20), and 25% of Andromed® (Andro25), respectively. The diluted-spermatozoa was placed in the refrigerator (5 °C) for three days. The quality of diluted-spermatozoa including percentages of progressive motility (PM), viability (VB), and intact plasma membrane (IPM) were evaluated every day for three days. The results showed that the PM, VB, and IPM of spermatozoa were reduced significantly during storage. At day-4 of storage, mean percentages of PM, VB, and IPM for Andro20 (26%, 42%, and 37,8%) was significantly (P<0.05) higher than control (21%, 34.8%, and 33.6%), Andro15 (11%, 25.8%, and 24.2%), and Andro25 (10%, 23.4%, and 26.4%). In conclusion, 20% Andromed® concentration in 80% bidestilled water is the best option of extender in term of the ability in maintain the quality of garut ram epididymal spermatozoa during storage at 5 °C.


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Preservation of epididymal spermatozoa of garut ram in soybean lechitin-based extender

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