Prevalence and antimicrobial susceptibility profile of MRSA and VRSA strains isolated from out – door patients of Swabi region, Pakistan

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Prevalence and antimicrobial susceptibility profile of MRSA and VRSA strains isolated from out – door patients of Swabi region, Pakistan

Johar Jamil, Khuzaifa Zaman, Iftikhar Ali, Saad Ullah, Faheem Ahmad Khan, Kalsoom, Noor Ullah, Zakir Ullah
Int. J. Biosci.14( 1), 156-161, January 2019.
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Staphylococcus aureus is one of the nosocomial pathogen, causes life threatening infection include urinary tract infection (UTI), blood stream infection and soft tissue infection. The purpose of this study was to detect Methicillin resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Staphylococcus aureus (VRSA) in outdoor patients based on antibiotic susceptibility profiling. Total of hundred different specimens i.e. pus, urine, blood and sputum were collected and analyzed in Microbiological laboratory for presence of Staphylococcus aureus. All specimens were inoculated on different media like MacConkey and MSA and incubated on 37°C. Disc diffusion method was used to check antimicrobial susceptible profile. Out of 100 samples Staphylococcus aureus was detected in 17 samples. Among these 17 positive samples four were MSSA, nine were both MRSA and VRSA and 4 isolates were VRSA. Among all isolates 64.71% were resistant of gentamycine, 23.53% to cefoxitin, 23.53% to vancomycin, 58.82% to erythromycin, 100% to pencilline and 52.94% to levofloxicin. Whereas all isolated strains were sensitive to linezolid only. It is concluded that MRSA and VRSA are prevalent in outdoor patients and linezolid is only antibiotic which is susceptible to all isolates.


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