Prevalence and their possible risk factors of hepatitis B Virus Infection in Bannu District of Khyber Pakhtunkhwa, Pakistan

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Research Paper 01/11/2018
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Prevalence and their possible risk factors of hepatitis B Virus Infection in Bannu District of Khyber Pakhtunkhwa, Pakistan

Adil Hassan, Irfan Ullah, Ahmad Ud Din, Muhammad Naveed, Zaheer ud Din, Abdul Majeed Khan, Azam Hayat
Int. J. Biosci.13( 5), 351-357, November 2018.
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Hepatitis B infection is the leading health problem throughout the globe. It is estimated that worldwide, the prevalence of HBV is approximately 5 percent, but the percentage varies globally. During this study 300 blood sample were collected from district Bannu and analyzed in District Headquarter Hospital, Bannu. The entire blood samples were initially tested by Immunochromatographic (ICT) kits, and the ICT positive samples were further assayed by polymerase chain reaction (PCR). Out of total three hundred blood samples, three major areas were selected and analyzed for HBV infection; the highest prevalence was recorded in Bannu city on ICT kits was 14.44% followed by Kakki 10.78% and Domail 8.88%. By PCR, the greater percentage i.e. 9.44% of HBV Infection was reported in Bannu followed by other study areas, and as for as the age wise distribution was concerned, the highest percentage was 8.49% in age between 21-40. In general population of Bannu, the male community showed the highest prevalence (8.8%) and lowest percentage was recorded in females. The evaluation and examination of the Hepatitis B infection from Bannu revealed that the proportion of the disease increases with the increase in age.


Alam M, Naeem MA. 2007. Frequency of hepatitis B surface antigen and anti-hepatitis Cantibodies in apparently healthy blood donors in Northern areas. Pakistan Journal of Pathology 18, 11–14.

Afzal MS, Khan MY, Ammar M, Anjum S, Zaidi NUSSS. 2014. Diagnostically untypable hepatitis C virus variants: It is time to resolve the problem. World Journal of Gastroenterology 20, 17690-17692.

Ali M, Idrees M, Ali L, Hussain A, Ur Rehman I, Saleem S, Afzal S, Butt S. 2011. Hepatitis B virus in Pakistan: A systematic review of prevalence, risk factors, awareness status and genotypes. Virology Journal 8, 102.

Bowyer S, Sim J. 2000. Relationships within and between genotypes of hepatitis B virus at points across the genome: footprints of recombination in certain isolates. Journal of General Virology 81, 379-392.

Bukhari SM, Khatoon N, Iqbal A, Naeem S, Shafqat S, Lone A. 1999. Prevalence of Hepatitis “B” Antigenemia in Mayo Hospital, Lahore. Biomedica 15, 88- 91.

Choudhary I, Khan S. 2005. Should we do Hepatitis-B and C screening on each patient before surgery. Pakistan Journal of Medical Science 21, 278-80.

Hollinger FB, Lau DTY. 2006. Hepatitis B: the pathway to recovery through treatment. Gastroenterology Clinics of North America 35, 425-461.

Heermann KH, Gerlich WH, Chudy M, Schaefer S, Thomssen R. 1999. Quantitative Detection of Hepatitis B Virus DNA in Two International Reference Plasma Preparations. Journal of Clinical Microbiology 37, 68-73.

Li G, Li W, Guo F, Xuc S, Zhaod N, Chena S, Liu L. 2010. A novel real-time PCR assay for determination of viral loads in person infected with hepatitis B virus. Journal of Virological Methods 165, 9–14.

Lai CL, Yuen MF. 2007. The natural history and treatment of chronic hepatitis B: a critical evaluation of standard treatment criteria and end points. Annals of Internal Medicine 147, 58-61.

Mahoney FJ, Kane M. 1999. Hepatitis B vaccine. In: Plotkin SA, Orenstein WA, editors. Vaccines. 3rd ed. Philadelphia: W.B. Saunders Company 158–82.

Mata MJA, Arroyo ACI, Calderón GM, Cazares RS, Fuentes AJL, Arias FR, Gaytán MJ. 2012. Prevalence and resistance pattern of genotype G and H in chronic hepatitis BandHIV co-infected patients in Mexico. Annals of Hepatology 11, 47-51.

Paraskevis D, Beloukas A, Haida C, Katsoulidou A, Moschidis Z, Hatzitheodorou H, Varaklioti A, Sypsa V, Hatzakis A. 2010. Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA. Virology Journal 7, 57.

MRC. National Survey on Prevalence of Hepatitis B & C in General Population of Pakistan Pakistan Medical Research Council, Shahra h-e-Jamhuriat, Sector G- 5/2, Islamabad. 2009.

PRauf A, Nadeem MS, Ali A, Iqbal M, Mustafa M, Latif MM, Latif MZ, Ahmed N, Shakoori AR. 2011. Prevalence of hepatitis B and C in internally displaced persons of war against terrorism in Swat, Pakistan. European Journal of Public Health 21, 638–642.

Workowski KA, Berman SM. 2002. CDC sexually transmitted diseases treatment guidelines, Oxford University Press.

Workowski KA. 2006. Sexually transmitted diseases treatment guidelines, 2006. MMWR recomm Rep 55, 1-94.

Zhu CT, Dong C. 2009. Characteristics of general distribution of hepatitis B virus genotypes in China. Hepatobiliary & Pancreatic Diseases International 8, 397-401.