Prevalence, incidence, severity and morphological characterization among isolates of karnal bunt (Tilletia indica) in Punjab and Khyber Pakhtunkhwa (Pakistan)

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Prevalence, incidence, severity and morphological characterization among isolates of karnal bunt (Tilletia indica) in Punjab and Khyber Pakhtunkhwa (Pakistan)

Aasma, Shahzad Asad, Muhammad Fayyaz, Anjum Munir
Int. J. Biosci.15( 2), 106-117, August 2019.
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Karnal bunt is a disease of stern concern all over the world and causing economic losses in wheat-growing areas of Pakistan. The disease is known to occur since long and is the main constraint in exporting the wheat in Pakistan. Scanty work on this disease is done in the past. So keeping in view its significance a study was designed to ascertain the disease situation in wheat production zones of Punjab and Khyber Pakhtunkhwa. The disease has shown its increasing trend with few available reports and the pathogen became established in the places where previously it was not known. The prevalence was calculated from both physically observed grains and wash test while isolations of the isolates were made only from damaged grains. During 2013-14 wheat season the average Prevalence, incidence and severity of Karnal bunt in Punjab were 4.87%, 0.66, 0-2 and 14.87%, 1.19 %, 0-2 respectively whereas in Khyber Pakhtunkhwa, it was found to be 11.33%, 3.02.0-2  and 15.06%, 3.49%, 0-2 respectively. In the current study detailed work on the morphological characterization of Tilletia indica was done as the scientific information on pathogen morphology is lacking in Pakistan. Variation was observed in the morphology of 49 isolates on the basis of teliospores size, percentage germination, size of filliform and allantiod sporida (secondary sporidia), growth pattern and colony color. Size of teliospores ranged from 53.09µm (PB-55) to 35.23 µm (PB-68). The germination ranged from 78.53 % (PB-50) to 10.82 % (KPK-17). Continuous monitoring is needed to identify the areas which are free from Karnal bunt pathogen.


Aasma Zakria M, Asad S, Jamal A, Fayyaz M, Atiq-ur-Rehman R, Munir A, Iftikhar S, Ahmad Y. 2012. Morphological and physiological characterization of Tilletia indica isolates from Punjab and Khyber Pakhtunkhwa. Pakistan Journal of Phytopathology 24, 106-111.

Aujla SS, Sharma I, Gill KS. 1986. Effect of time and method of inoculation on Karnal bunt development. Indian Phytopathology 39, 230-33.

Aujla SS, Sharma I, Singh BB.1989. Rating scale for identifying of wheat varieties resistant to Neovossia indica. Indian Phytopathology 42, 161-162.

Bansal R, Singh DV, Joshi LM. 1984. Effect of Karnal-bunt pathogen [Neovossia indica (Mitra) Mundkur] on weight and viability of wheat seed. Indian Journal of agricultural sciences 54, 663-666.

Bedi SK, Sikka MR.1949. Transmission of wheat bunt due to Neovossia indica (Mitra) Mundkur. Indian Phytopathology 2, 20-6.

Begum S, Mathur SB. 1989. Karnal bunt and loose smut in wheat seed lots of Pakistan. FAO Plant Protect Bulletin 37, 165-173.

Bhatti MA, Ilyas MB. 1986. Wheat diseases in Pakistan.Problems and progress of wheat pathology in South Asia 401.

Bonde MR, Smilanick JL. 1998. Life cycle and environmental requirements of Tilletia indica. InProc. Bunts and Smuts of Wheat: An International Symposium 17-20.

Carris LM, Castlebury LA, Goates BJ. 2006. Nonsystemic bunt fungi—Tilletia indica and T. horrida: a review of history, systematics, and biology. Annu. Rev. Phytopathology 44, 113-33.

Gill KS, Indu S, Aujla SS. 1993. Karnal bunt and wheat production. Punjab Agricultural University, Ludhiana, India 1-153.

Government of Pakistan. 2018. Economic Survey, Ministry of National Food security and research, Pakistan Bureau of Statistics, Pakistan 17.

Ingold CT. 1997. The basidium of Tilletia and its evolution. Mycologist 11, 98-100.

Jones GD, Clifford BC. 1978. Cereal diseases their pathology and control. BASF United Kingdom Limited, Agrochemical Division, Ipswich, Suffolk 279.

Joshi LM. 1988. Plant pathological problems in India as exemplified by Karnal bunt of wheat.Indian Journal of Mycology and Plant Pathology 17, 11-21.

Joshi LM, Singh DV, Srivastava KD. 1980. Present status of karnal bunt in India. Indian Phytopathology 33, 147-148.

Khan JS, Jalaluddin M, Ghaffar. 1992. Major seed borne diseases of wheat in Pakistan. In: Status of Plant pathology in Pakistan. Proc. Nat. Sym.(Eds.) A. Ghaffar& S. Shahzad, Dept. of Botany, University of Karachi115.

Khan JS, Naseer N, Jalauddin M. 2005.Occurrence of major diseases of wheat under different agro climatic zones of Pakistan. Pakistan Journal of Biological Sciences 8, 356-60.

Khanna A, Payak MM, Mehta SC. 1968. Teliospore morphology of some smut fungi. I. Electron microscopy. Mycologia 58, 562-9.

Kumar J, Saharan MS, Sharma AK, SHARMA S, Nagarajan S. 2004.Pathogenic and molecular variation among Indian isolated of Tilletia indica causing Karnal bunt of wheat. Indian Phytopathology 57, 144-149.

Mathur SB, Cunfer BM. 1993.Seed-borne diseases and seed health testing of wheat.Danish Government Institute of Seed Pathology for Developing Countries 168.

Mirza JI. 2005.  Identification of sources of resistance to Karnal bunt disease of wheat ALP-Wheat Umbrella Component-IV. Final Progress Report of the. Crop Disease Research Program, Institut of Plant and Environmental Protection. National Agricultural Research Centre Islamabad 1-40.

Mitra M. 1935. Stinking smut (bunt) of wheat with special reference to Tilletia indica. Indian Journal of Agricultural Sciences 5, 51-74.

Mundkur BB.1943. Karnal bunt, an air-borne disease.Current Science 13, 230-1.

Pannu PP, Chahal SS. 2000.Variability in Tilletia indica, the incitant of Karnal bunt of wheat.Indian Phytopathology 53, 279-82.

Raees A, Riaz A, Zakria M, Naz F. 2013. Incidence of Karnal bunt (Tilletia indica Mitra) of wheat (Triticumaestivum L.) in two districts of Punjab (Pakistan) and identification of resistance sources. Pakistan Journal of Phytopathology 1, 1-6.

Sajjad M, Saeed A, Muhammad AH, Hafiz  M, Zia UG, Muhammad N, Muhammad F, Manzoor H. 2018. Incidence of Karnal bunt (Tilletia indica Mitra) of wheat in southern Punjab, Pakistan. International Journal of Biosciences 12, 280-285.

Saleem A, Akhtar KM. 1988. Karnal bunt of wheat in Pakistan.National Seminar on the role of plant health and care in Agricultural Production, 28-29.

Shakoor MA. 2009.  A disease predictive model for chemotherapy of Karnal bunt of wheat (Doctoral dissertation, University of agriculture, Faisalabad).

Sharma R, Kumar R. 2017. Karnal bunt disease of wheat study from Jhunjhunu, Rajasthan. International Journal of Advance Research, Ideas and Innovations in Technology 3, 834–835.

Sharma I, Nanda GS, Kaloty PK. 1998. Variability in Neovossia indica: based on pathogenecity and isozyme analysis. Tropical Agricultural Research and Extension 1, 159–161.

Shetty SA, Aruna K, Shetty HS. 1988. Investigations on kernel smut of paddy. Plant Disease Research 4, 172-6.

Singh DV, Srivastava KD, Aggarwal R, Jain SK. 1996. Factors associated with development and spread of Karnal bunt of wheat (Triticumaestivum) in north-western India. Indian Journal of Agricultural Sciences 66, 374-83.

Singh DV, Joshi LM, Srivastava KD. 1983. Karnal bunt–a new threat to wheat in India. Recent advances in plant pathology: Prof. HK Saksena Festschrift/[edited by]Hussain A, Singh K, Singh B P and Agnihotri V P (eds.)Print House, Lukhnow, India, 121-135.

Singh S, Gill KS, Dhaliwal HS, Singh H, Gill BS. 1994. Towards molecular tagging of Karnal bunt resistance gene (s) in wheat. Journal of Plant Biochemistry and Biotechnology 3, 79-83.

Singh M, Singh A.1998.Variability of Tilletia indica, the causal fungus of karnal bunt of wheat.Acta phytopathologica et entomologica hungarica 33, 323-33.

Singh PP, Bedi PS. 1985. Effect of’Karnalbunt infection on gluten characteristics and protein fractionation of wheat grains.Annals of Biology 1, 223-5.

Warham EJ, Mujeeb-Kazi A, Rosas V. 1986. Karnal bunt (Tilletia indica) resistance screening of Aegilops species and their practical utilization for Triticumaestivum improvement. Canadian Journal of Plant Pathology 8, 65-70.

Wright D, Murray G, Brennan J, Tan MK. 2013. Draft National contingency plan for Karnal Bunt of Wheat. Part IV Diagnostic Protocols.Plant Health Australia.