Prevalence of Eimeria bovis in cattles of Cholistan desert, Pakistan

Paper Details

Research Paper 01/08/2016
Views (175) Download (6)

Prevalence of Eimeria bovis in cattles of Cholistan desert, Pakistan

Syeda Rida Zainab, Mirza Imran Shahzad, Muhammad Zahid Mustafa, Maryam Arshad, Tahira Ruby
J. Bio. Env. Sci.9( 2), 94-98, August 2016.
Certificate: JBES 2016 [Generate Certificate]


Protozoan parasites are responsible for a number of diseases in livestock thus affecting a country economy. The coccidiosis is very common parasitic disease in livestock of Pakistan especially in desert animals. A valid procedure was used to detect E. bovisin fecal samples of cholistanicow. PCR reaction was performed using species specific primer for amplification of ITS-1 region of rRNA gene. E. bovis was confirmed in fecal samples obtained cholistani cow while E. zuernii was absent. The PCR based detection of Eimeria parasites seemed highly sensitive when compared with traditional morphological examination of oocytes.


Akhtar M, Anwar MI, Iqbal Z, Muhammad F, Awais MM, Haq AU, Sawicka HE. 2012. Immunological evaluation of two local isolates of Eimeria tenella gametocytes against coccidiosis in poultry. Pakistan veterinary Journal 32, 77-80.

Ali I, Chauhdry MS, Farooq U. 2009. Camel rearing in Cholistan desert of Pakistan. Pakistan veterinary Journal.  29, 85-92.

Costa CA, Gomes RF, Melo MN, Ribeiro MF. 2001. Eimeria parasite of domestic fowl, genetic relationship of different isolates estimated from RAPD-DNA. Parasitology Research 87, 459-456.

Hoge CW, Shlim DR, Ghimire M. 1995. Placebo-controlled trial of cotrimoxazole for cyclospora infections among travellers and foreign residents in Nepal. Lancet 345, 691-693. › NCBI › Literature › PubMed Central (PMC).

Kausiluka LJM, Kambarage DM, Matthewman RW, Harrison LHS, Daborn CJ. 1996. Coccidiosis in small ruminants in Tanzania. Small Ruminant Research 21, 127-131.

Kawahara F, Taira K, Nagai S, Onaga H, Onuma M, Nunoya T. 2008. Detection of five avian Eimeria species by species-specific realtime polymerase chain reaction assay. Avian Diseases 52, 652-656. 19166058.

Kawaharaa F, Zhanga G, Mingalab CN, Tamurac Y, Koiwac M, Onumaa M, Nunoyaa T. 2010. Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites. Veterinary Parasitology 174, 49-57.

Khan IA, Khan A, Hussain A, Riaz A, Aziz A. 2011. Hemato-biochemical alterations in crossbred cattle affected with bovine theileriosis in semiarid zone. Pakistan Veterinary Journal 31, 137-140.

Levine ND, Ivens V. 1970. The Coccidian Parasites (Protozoa, Sporozoa) of Ruminants. Illinois Biology Monograph No. 44. University of Illinois Press, London.

Li G, Xiao S, Zhou R, Li W, Wadeh H. 2007. Molecular characterization of Cyclospora-like organism from dairy cattle. Parasitology Research 100, 955-961.

Martynova VA, Syvyk A, Teplova I, Hume M, Nalian A. 2010. Rapid detection of avian Eimeria species using denaturing gradient gel electrophoresis. Veterinary Parasitology 174, 49-57. www.

Nalbantoglu S, Sari B, Cicek H, Karaer Z. 2008. Prevalence of coccidian species in the water buffalo (Bubalusbubalis) in the Province of Afyon, Turkey. Acta Veterinaria Brno 77, 111-116.actavet. › Archive › 2008, Vol. 77 › Issue 1.

Olsen GJ, Woese CR. 1993. Ribosomal RNA: a key to phylogeny. FASEB J 7, 113-123. www.ncbi.nlm.nih. gov/pubmed/8422957.

Om H, Kumar S, Singh P. 2010. Prevalence of coccidia in Mathura region of Uttar Pradesh. Veterinary World 3, 503-505.

Raza MA, Younus M, Schlecht E. 2014. Prevalence of gastrointestinal helminths in pastoral sheep and goat flocks in the Cholistan desert of Pakistan. Journal of Animal and Plant Sciences 24, 127-134.

Schnitzler BE, Thebo PL, Mattsson JG, Tomley FM, Shirley MW. 1998. Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic Eimeria species of the chicken. Avian Pathology 27, 490-497.

Schnitzler BE, Thebo PL, Tomley FM, Uggla A, Shirley MW. 1999. PCR identification of chicken Eimeriaa simplified read-out. Avian Pathology 28, 89-93.

Woods WG, Whithear KG, Richard DG, Anderson GR, Jorjensen WK, Gasser RB. 2000. Single strand RFLP analysis of ITS-2 fragment for six species of Eimeria from chickens in Australia. International Journalof Parasitology 30, 1019-1023.