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Research Paper | April 10, 2022

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Prevalence of extended spectrum beta lactamases and AmpC producing gram negative bacilli among surgical site infections at a Tertiary Care Hospital

A. Divya, Pramod N. Sambrani, Uma Chikkaraddi

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Int. J. Micro. Myco.14(4), 1-9, April 2022

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Abstract

Infection control professionals, Clinicians, Microbiologists are concerned about Gram negative bacteria producing Extended spectrum Beta lactamases (ESBL) and AmpC because they pose a challenge of effective antimicrobial therapy resulting in adverse patient outcomes. The objective of the present study is to know the prevalence of ESBL and AmpC thereby making appropriate changes in the choice of antimicrobial therapy and minimizing treatment failures. To know the prevalence of Extended Spectrum Beta Lactamases and AmpC producers among Gram Negative isolates from pus samples suspected of Surgical-Site Infections (SSI). This study is a prospective cross-sectional study. A total of 100 samples from wounds in General Surgery, Obstetrics Obstetrics-Gynaecology, Orthopaedic, ENT and Ophthalmology departments, which were suspected of surgical site infection submitted to the Microbiology Laboratory of Karnataka Institute of Medical Sciences, were included in the study. Out of the total 100 cases, 17 (21.3%) were ESBL producers and Escherichia coli was the most common ESBL producing bacteria. 31 (38.8%) were AmpC producers and Klebsiella species were the most common AmpC producing bacteria. 27 (33.8%) were both ESBL and AmpC producers/ Co-producers. In the present study, we found an increasing number (21.3%) of ESBL and (38.8%) AmpC producing Gram negative isolates. ESBL and AmpC producing strains were found to show higher rates of resistance to various class of antibiotics when compared to non ESBL and non AmpC producers. The indiscriminate use of cephalosporins should be limited, which helps to minimize the emergence of resistance.

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Prevalence of extended spectrum beta lactamases and AmpC producing gram negative bacilli among surgical site infections at a Tertiary Care Hospital

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