Prevalence of multidrug resistant bacteria in Rawal lake and its catchment area-an alarming situation for Rawalpindi city

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Research Paper 01/06/2017
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Prevalence of multidrug resistant bacteria in Rawal lake and its catchment area-an alarming situation for Rawalpindi city

Maqsood Ahmad, Muhammad Kamran, Muhammad Farhan, Zahid Ali Butt, Naima Raza, Muhammad Ashraf, Abdul Wahid
Int. J. Biosci.10( 6), 216-222, June 2017.
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Water samples were taken from Rawal Lake and its catchment area during January and August to study seasonal impact on prevalence of antibiotic resistant bacteria namely Escherichia coli (E. coli), Salmonella typhi (S. typhi) and Klebsiellap neumonia (Klebsiella) in the aquatic environment. The Rawal Lake and its whole catchment area were contaminated with the pathogenic bacteria except the two tributaries away from human population and activities. Results of the investigation revealed that isolates of E. coli, S. typhi and Klebsiella collected during August and January were 45 to 100% and 40 to 90% found antibiotic resistant respectively. This difference may be due season depended human activities and favorable temperature. Selected isolates of the three bacteria were examined for Erythromycin (Ert), Kanamycin (Kan), Ciprofloxacin (Cip), Norfloxacin (Nor), Ofloxacin (Oxl), Ampicillin (Amp), Vancomycin (Van), Amoxicillin (Amx). No isolate was resistant to less than four antibiotics understudy, highest resistance was shown by isolate of E. coli named as EC6 which was resistant to all the eight antibiotics. Untreated wastewater from recreational sites, hotels and hospital entering into the catchment area was mainly observed responsible for the resistant bacteria.


Ahmad M, Khan AU, Wahid A, Ali AS, Ahmad F. 2013. Role of untreated waste water in spread of antibiotics and antibiotic resistant bacteria in River. Pakistan Journal of Science 65(1), 10-15.

Alanis AJ. 2005. Resistance to Antibiotics: Are We in the Post-Antibiotic Era? Archives of Medical Research 36, 697-705.

Ash RJ, Brena M, Melissa M. 2002. Antibiotic Resistance of Gram-Negative Bacteria in Rivers, United States. Emerging Infectious Diseases 8(7), 116-122.

Blasco MD, Esteve C, Alcaide E. 2008. Multiresistant waterborne pathogens isolated from water reservoirs and cooling systems. Journal of Applied Microbiology 105, 469-475.

Clark HE, Geldrich EF, Kabler PW, Huff CB. 1958. Applied microbiology. International book company, New York pp. 27-33.

Fumie A, Atsushi Y, Koh-Ichi T, Ryuji K. 2013. Occurrence of fluoroquinolones and fluoroquinolone-resistance genes in the aquatic environment. Science of the Total Environment 444, 508-514.

Gunnar A, Charlotta E, Andreas H. 2011. The global need for effective antibiotics – A summary of plenary presentations. Drug Resistance Updates 14, 70–76.

Hocquet D, Muller A, Bertrand X. 2016. What happens in hospitals does not stay in hospitals: antibiotic-resistant bacteria in hospital wastewater systems. Journal of Hospital Infection 93, 395-402.

Hori S, Ohshita Y, Utsui Y. Hiramatsu K. 1993. Sequential acquisition of norfloxacin and ofloxacin resistance by methicillin-resistant and -susceptible Staphylococcus aureus. Antimicrobial agents chemotherapy 37, 2278-2284.

Huang JJ, Lu SQ, Li Y, Tang F, Lu Y, Wei B. 2012. Monitoring and evaluation of antibiotic-resistant bacteria at a municipal wastewater treatment plant in China. Environment International 42, 31-36.

Jones-Lepp TL, Sanchez C, Alvarez DA, Wilson DC, Taniguchi-Fu RL. 2012. Point sources of emerging contaminants along the Colorado River Basin: Source water for the arid Southwestern United States. Science of the Total Environment 430, 237-245.

Koczura R, Mokracka J, Jablonska L, Gozdecka E, Kubek M, Kaznowski A. 2012. Antimicrobial resistance of integron-harboring Escherichia coli isolates from clinical samples, wastewater treatment plant and river water. Science of the Total Environment 414, 680-685.

Kummerer K. 2009. Antibiotics in the aquatic environment. A review-Part-I. Chemosphere 75, 417-434.

Larsson  DG, Pedro C, Paxeus N. 2007. Effluent from drug manufactures contains extremely high levels of pharmaceuticals. Journal of Hazardous Materials 148, 751-755.

Lucia B, Tomas M, Igor B, Jozef R, Jaroslav S. 2014. Pilot study of seasonal occurrence and distribution of antibiotics and drug resistant bacteria in wastewater treatment plants in Slovakia. Science of the Total Environment 490, 440-444.

Maqsood A, Amin UK, Abdul W, Farhan M, Zahid AB, Farooq A. 2014. Urban Wastewater as Hotspot for Antibiotic and Antibiotic Resistant Bacteria Spread into the Aquatic Environment. Asian Journal of Chemistry 26(2), 579-582.

Medinet. 2013. php?option=com_content&view=article&id=81&Itemid=60.

Munir M,  Wong K, Xagoraraki I. 2011. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan. Water Research 45, 681-693.

Roychoudhury S, Catrenich EC, Mcintosh EJ, Mckeever HD, Makin KM, Koenigs PM, Ledoussal B. 2001. Quinolone resistance in staphylococci: Activities of new nonfluorinated quinolones against molecular targets in whole cells and clinical isolates. Antimicrobial Agents and Chemotherapy 45 (4), 1115-1120.

Schmieder R, Edwards R. 2012. Insights into antibiotic resistance through metagenomic approaches. Future Microbiology, 7(1), 73-89.

Scott B, Raj B, Rajkumar N, Angie C, Gary L. 2015. Presence of antibiotic resistant bacteria and antibiotic resistance genes in raw source water and treated drinking water. International  Biodeterioration & Biodegradation 102, 370–374.

Shoaib MH. 2007. Pharmacokinetic and physico-chemical studies on oral cephradine preparations. Ph.D Thesis, Department of Pharmaceuticals, University of Karachi.

Sim WJ, Lee J, Lee ES, Shin SK, Hwang SR, Oh JE. 2011. Occurrence and distribution of pharmaceuticals in wastewater from households, livestock farms, hospitals and pharmaceutical manufactures. Chemosphere 82, 179-186.

Stepanauskas R, Glenn TC, Jagoe CH, Tuckfield RC, Lindell AH, King CJ. 2006. Coselection for microbial resistance to metals and antibiotics in freshwater microcosms. Environmental Microbiology 8, 1510-1514.

Zhang YJ, Geiben SU, Gal C. 2008. Carbamazepine and diclofenac: removal in wastewater treatment plants and occurrence in water bodies. Chemosphere 73, 1151-1161.

Zheng S, Qiu X, Chen B, Yu X, Liu Z, Zhong G, Li H, Chen M, Sun G, Huang H, Yu W, Freestone D. 2011. Antibiotics pollution in Jiulong River estuary: Source, distribution and bacterial resistance. Chemosphere 84, 1677-1685.

Zheng S, Qiu X, Chen B, Yu X, Liu Z, Zhong G, Li H, Chen M, Sun G, Huang H, Yu W, Freestone D. 2011. Antibiotics pollution in Jiulong River estuary: Source, distribution and bacterial resistance. Chemosphere 84, 1677-1685.