Prevalence of tuberculosis in Khyber Pakhtunkhwa Pakistan using GeneXpert assay and light microscopy

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Research Paper 01/10/2019
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Prevalence of tuberculosis in Khyber Pakhtunkhwa Pakistan using GeneXpert assay and light microscopy

Kiran Farooq, Aurangzeb, Mohibullah Shah, Muhammad Sameem Javed, Adnan Amjad, Waliullah Khan, Rishma, Sahib Gul Afridi
Int. J. Biosci.15( 4), 223-229, October 2019.
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Tuberculosis (TB) is a common disease in developing countries including Pakistan which ranks sixth amongst highest tuberculosis reported countries. The present study aimed to determine the frequency of TB in Peshawar, Mardan and Swabi regions of Khyber Pakhtunkhwa province in Pakistan and also to evaluate the comparative performance of GeneXpert and microscopy analysis. In this cross-sectional study363 patients were included from Mardan (270), Nowshera (42) and Swabi (51). An early morning deep cough sputum of about 2ml was collected from each patient. Two aliquots of each sample were prepared one each for the analysis through GeneXpert and ZN staining based light microscopy. Among 363 cases 207 (57%) were males and 156 (43%) were females. The light microscopy method confirmed 198 (55%) while GeneXpert MTB/RIF assay confirmed 234 (64.4%) samples positive for Tuberculosis. The sensitivity of light microscopy was found 84.6% whereas for GeneXpert MTB/RIF it was 98%. Specificity of light microscopy and GeneXpert were found almost the same. Among the reported positive cases, Mardan constituted 74% of the cases while Nowshera and Swabi had 12% and 14% cases, respectively. Moreover, 83% of the cases had family history of tuberculosis. Maximum number of patients (117) was observed in the age group of 26-40 years. Frequency of TB was higher in Mardan than Nowshera and Swabi. GeneXpert method proved to be more efficient and sensitive.


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