Probiotic behavior study of four Enterococcus faecium isolated from raw milk in Algeria

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Research Paper 01/01/2020
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Probiotic behavior study of four Enterococcus faecium isolated from raw milk in Algeria

Loumani Akil, Larbi Ahmed Amine, Bekada Ahmed Mohamed Ali, Bensoltane Ahmed
Int. J. Biosci. 16(1), 301-307, January 2020.
Copyright Statement: Copyright 2020; The Author(s).
License: CC BY-NC 4.0

Abstract

Lactic acid bacteria (LAB) were isolated from spontaneously fermented raw goat milk collected from Relizane, Algeria, and assessed for their probiotic properties. Four acid-tolerant strains were selected. By using 16S rDNA sequencing, these isolates were confirmed to be Enterococcus faecium. The strains exhibited good survivability in a highly acidic environment at pH 2 and in bile salts at concentrations of 0.3% and 2%. The strains were susceptible to antibiotics except colistin and were found to inhibit the growth of Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Salmonella Typhi. The strains were nonhemolytic and showed weak production of exopolysaccharides (EPSs). The study confirmed that all the identified strains have in vitro probiotic properties.

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