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Production of eleutherosides, total phenolics and total favonoids from somatic embryos of Siberian ginseng affected by different aeration volume in bioreactor

Research Paper | April 1, 2013

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Abdullah Mohammad Shohael, Kee Yoeup Paek

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Int. J. Biosci.3( 4), 213-221, April 2013

DOI: http://dx.doi.org/10.12692/ijb/3.4.213-221


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Physical culture condition plays crucial roles in mass production of somatic embryos in bioreactor. The effects of supplied aeration volume in a balloon type bubble bioreactor (BTBB) to produce somatic embryos were investigated in this study. Best response in case of fresh weight (FW), dry weight (DW) and growth ratio (GR) were observed when input aeration volume were changed every 10 days as follows 0.05/0.1/0.2/0.3 vvm rather than constant aeration volume. Maximum FW 99.28 g/l and DW 11.35 g/l were found in above aeration volume although the highest growth ratio was noticed when 0.05 vvm constant aeration volume was applied. Highest amount of total eleutherosides (108.51 µg/g DW) were produced while aeration volume was changed in every 10 days but higher chlorogenic acid was found while constant aeration volume was 0.01 vvm (1.05 mg/g DW). Pattern of total phenolics and total flavonoids production also revealed that, maximum amount of those metabolities were produced when input air was changed every 10 days. The results indicated that input aeration volume has a potential role on somatic embryos production and secondary metabolites accumulation in bioreactor.


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Production of eleutherosides, total phenolics and total favonoids from somatic embryos of Siberian ginseng affected by different aeration volume in bioreactor

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