Progressive impact of alternaria blight on antioxidants enzyme of mustard leaves after infection of Alternaria brassicae to induce resistance

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Research Paper 01/10/2018
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Progressive impact of alternaria blight on antioxidants enzyme of mustard leaves after infection of Alternaria brassicae to induce resistance

Ahmed Subhani, Muhammad Asif, Muhammad Atiq, Muhammad Imran, Akhtar Hameed, Abdul Qadus, Shafqat Ali, Adeel Sultan, Muhammad Akmal, Muhammad Hafeez ul Haq, Umar Farooq, Nasir Ahmed Rajput
Int. J. Biosci.13( 4), 417-426, October 2018.
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Mustard (brassica) is second most important oil seed crop which belongs to family brassicaeae. It contributes 17% in domestic oil production. Alternaria is a very lethal disease which is caused by Alternaria brassicaeae. It causes 47% yield losses. Brassica napus is infected by Alternaria blight which is responsible to cause variations in its biomolecules and antioxidant profile. The research was undertaken to study variation in biochemistry of resistance associated enzymatic profile of brassica germplasm. Pot experiment was designed on such fifteen varieties in greenhouse. Inoculum was prepared to inoculate these varieties and healthy leaves were collected along with inoculated showing symptoms of disease. Collected samples were analyzed using spectrophotometer. Results showed that Rapid decrease or reduction in catalase and SOD activity was observed in inoculated leaves of moderately susceptible varieties Punjab canola (0.0373) and Faisal canola (0.069), (0.211 and 0.097) to (0.077 and 0.082) respectively. Overall varieties depicted increasing trend but commonly H2O2 and POD in moderately susceptible cultivars was slightly decreased from (0.0587, 0.2350) to (0.0530, 0.1973). In contrast drastic increase was found in highly susceptible varieties while it was obvious that behavior of susceptible to highly susceptible lines was almost same. Highly susceptible lines showed slight decrease comparable to susceptible one. Protein activity was found to increase in inoculated leaves of all varieties. Phenolic activity in moderately Susceptible lines (Punjab Canola and Faisal Canola) rapidly and suddenly climbed up followed by susceptible cultivars. Data showed the smooth change protein and phenolic activity. In highly susceptible varieties (DGL, BSA and Toria selection A) phenolic level was (1.451, 1.344, 1.142) as compared to uninoculated leaves having (0.961, 0.816, 2.273 and 1.344) protein. It can be concluded that bio- antioxidant profile variations are useful tool to serve as effective marker for resistant germplasm documentation.


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