Propagation protocol of Pteris vittata L. using spores for phytoremediation

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Research Paper 01/06/2016
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Propagation protocol of Pteris vittata L. using spores for phytoremediation

Dennis A. Apuan, Mary Jean B. Apuan, Teresita R. Perez, Ruby Ellaine Perez, Rene Juna R. Claveria, Augustine Doronila, Mars Tan
Int. J. Biosci. 8(6), 14-21, June 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

Pteris vittata has been reported to be a hyperaccumulator of arsenic, and therefore useful in phytoremediation to clean-up arsenic-contaminated soils. Although factors for successful germination of spores have been identified, but information concerning the whole range of steps involved in mass propagating the fern is lacking. Thus, the study was aimed to develop a propagation protocol using spores to produce large number of seedlings. We implemented descriptive research methodologies such as observation and documentation of fern propagation activities in the nursery and conducted experiment following completely randomized designed with three replications. Here, we show that seedlings of Pteris vittata were successfully produced and protocol for its propagation is described.

Ayrapetov A, Ganger MT. 2009. Nutrient levels do not affect male gametophyte induction by antheridiogen  in  Ceratopteris  richardii. American Fern Journal 99, 273-278. http://dx.doi.org/10.1640/0002-8444-99.4.273

Brum FR, Randi AM. 2002.  High irradiance and temperature inhibit the germination of spores of the fern Rumohra adiantiformis (Forst.) Ching (Dryopteridaceae). Revista Brasilian Botany 25, 391-396. http://dx.doi.org/10.1590/S010084042002012000002

Chen T, Wei C, Huang Z, Huang Q, Lu Q, Fan Z. 2002. Arsenic hyperaccumulator Pteris vittata L. and its arsenic hyperaccumulation. Chinese Science Bulletin 47, 902-905. http://dx.doi.org/10.1360/02tb9202

Gonçalves dos Santos EP, Lehmann DRM, Santos M, Randi ÁM. 2010. Spore germination of Gleichenella pectinata (Willd.) Ching (Polypodiopsida-Gleicheniaceae) at different temperatures, levels of light and pH. Brazilian Archive of Biology and Technology 53, 1309-1318. http://dx.doi.org/10.1590/S15168913201000060000 7

Harvey R. 1993. The Fern Life Cycle. http://www.cpbr.gov.au/ferns/fern.spore.prop.html.

Kertulis-Tartar GM, Ma LQ, Tu C, Chirenje T. 2006. Phytoremediation of an arsenic-contaminated site using Pteris vittata L.:A Two-year study. International  Journal  of  Phytoremediation  8, 311-322. http://dx.doi.org/10.1080/15226510600992873

Lombi E, Zhao FJ, Fuhrmann M, Ma LQ, McGrath SP. 2002. Arsenic distribution and speciation in the fronds of the hyperaccumulator Pteris vittata. New Phytologist 156, 195-203. http://dx.doi.org/10.1046/j.1469-8137.2002.00512

Ma LQ, Komar KM, Tu C, Zhang W, Cai Y, Kennelley ED. 2001. A fern that hyperaccumulates arsenic. Nature 409, 579. http://dx.doi.org/10.1038/35054664

Nester J, Coolbaugh RC. 1986. Factors influencing spore germination and early gametophyte development in Anemia mexicana and Anemia phyllitidis. Journal of Plant Physiology 82, 230-235. http://dx.doi.org/10.1104/pp.82.1.230

Niazi NK, Singh B, Zwieten LV, Kachenko AG. 2011. Phytoremediation potential of Pityrogramma calomelanos var. Austroamericana and Pteris vittata L. grown at highly variable arsenic contaminated site. International Journal of Phytoremediation 13, 912-932. http://dx.doi.org/10.1080/15226514.2011.8023

Sakamaki Y, Ino Y. 1999.  Contribution of fern gametophytes to the growth of produced sporophytes on the basis of carbon gain. Ecological Research 14, 59-69. http://dx.doi.org/10.1046/j.1440-1703.1999.141277

Tu C, Ma LQ. 2002. Effects of arsenic concentration and forms on arsenic uptake by the hyperaccumulator ladder brake. Journal of Environmental Quality 31, 641-647. http://dx.doi.org/10.2134/jeq2002.0641

Tu C, Ma LQ, Bondada B. 2002. Arsenic accumulation in the hyperaccumulator Chinese brake and its utilization potential for phytoremediation. Journal of Environmental Quality 31, 1671-1675. http://dx.doi.org/10.2134/jeq2002.1671

Wan XM, Lei M, Huang ZC, Chen TB, Liu YR. 2010. Sexual propagation of Pteris vittata L. influenced by pH, calcium and temperature. International Journal of Phytoremediation, 12, 85-95. http://dx.doi.org/10.1080/15226510902767148

Wei CY, Sun X, Wang C, Wang WY. 2006. Factors influencing arsenic accumulation by Pteris vittata: a comparative field study at two sites. Environmental Pollution 141, 488-493. http://dx.doi.org/10.1016/j.envpol.2005. 08.060

Zhang W, Cai Y, Tu C, Ma LQ. 2002. Arsenic speciation and distribution in an arsenic hyperaccumulating plant. The Science of the Total Environment. 300, 167-177. http://dx.doi.org/10.1016/s0048-9697(02)00165-1

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