Protection effects of basil on cardiac cells apoptosis after chronic exposure to electromagnetic field (EMF) in rats by TUNEL assay
Paper Details
Protection effects of basil on cardiac cells apoptosis after chronic exposure to electromagnetic field (EMF) in rats by TUNEL assay
Abstract
With developing in electronic means and increase in using these concern about the public health hazards of chronic exposure to electromagnetic field has gained more attention Medicinal use of basil belong to ancient medicine in asia such as Iran. Wistar male rat (n=40) were allocated into four groups, control (n=10) and test groups (n=30), that subdivided into groups of 3, the extract group were received of basil extract (1.5g/kg body), second extract group were received of basil extract (1.5g/kg body) and emf group that exposed to50 Hz for 40 consequence day. In end of study the heart tissue of rats in whole groups were removed and prepared for pathology analysis by tunel method. Serum MDA, percentage of apoptotic cells and artery hyperemia significantly were increased in experimental group that has exposed to 50Hz EMF(p<0.05).the level of TAC in groups which received 1.5g/kg body basil extract significantly were increased (p<0.05) in comparison to control group. Morphology of heart in both experimental and control group were similar. Results revealed that administration of 1.5g/kg body of basil extract significantly decreased the apoptotic rate and protects cells by presents its antioxidant role.
Andrzejakr R, Poreba R, Poreba M, Derkacz A, Robert S, Pawel G, Boguslaw B, Steinmetz-Beck A, Pilecki W. 2008. The Influence of the Call with a Mobile Phone on Heart Rate Variability Parameters in Healthy Volunteers. Industrial Health 46, 409–417.
Arora R, Gupta D, Chawla R, Sagar, Sharma A, Kumar R, Prasad J, Singh S, Samanta N, Kumar SR. 2005. Radioprotection by Plant Products: Present Status and Future Prospects. Phytother Res. 19, 1–22 .
Basilico FC. 1999. Cardiovascular disease in athletes. Am J Sports Med. 27, 108-121.
Bellieni CV, Acampa M, Maffei M, Maffei S, Perrone S, Pinto I, Stacchini N, Buonocore G. 2008. Electromagnetic fields produced by incubators influence heart rate variability in newborns. Arch Dis Child Fetal Neonatal Ed, 132738.
Blettner M, Schlehofer B, Breckenkamp J. 2009. Mobile phone base stations and adverse health effects: phase 1 of a population-based, cross-sectional study in Germany. Occupational and Environmental Medicine 66(2), 118-123.
Erogul O, Oztas E, Yildirim , Kir T, Aydur E, Komesli G,Irkilata H C,limak MK, Peker AF. 2006. Effects of electromagnetic radiation from cellular phone on human sperm motility: an in vitro study. Arc Med Res. 37, 840-3.
Frahm J, Lantow M, Lupke M, Weiss DG, Simko M. 2006. Alteration in cellular functions in mouse macrophages after exposure to 50 Hz magnetic fields. J Cell Biochem. 99, 168-177.
Gülçin I, Elias R, Gepdiremen A, Boyer L. 2006. Antioxidant activity of lignans rom fringe tree (Chionanthus virginicus L ). Eur. Food Res. Technol. 223, 759–767.
Gülçin I, Elmastat M, Y. Aboul-Enein H. 2007. Determination of Antioxidant and Radical Scavenging Activity of Basil (Ocimum basilicum L. Family Lamiaceae) Assayed by Different Methodologies”. PHYTOTHERAPY RESEARCH Phytother Res. 21, 354–361.
Hasani-Ranjbar SH, Larijani B, Abdollahi M. 2009. A Systematic Review of the Potential Herbal Sources of Future Effective Drugs in Oxidant-Related Diseases. Inflammation & Allergy – Drug Targets. 8, 2-10 .
Irmak MK, Fadillioglu E, Gule CM,Erdogan H, Yagmurca M, Akyol O. 2002. Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits. Cell Biochem. Funct. 20, 279—283.
Jeong JH, Kim JS, Lee BC, Min YS, Kim DS, Ryu JS, Soh KS, Seo KM , Sohn UD. 2004. Influence of exposure to electromagnetic field on the cardiovascular system”.Autonomic & Autacoid, Pharmacology 25, 23-17.
Jauchem JR. 2007. Effects of low-level radio-frequency (3 kHz to 300GHz) energy on human cardiovascular, reproductive, immune, and other systems: A review of the recent literature”.Int. J. Hyg. Environ. Health. 211, 1–29.
Jauchem JR, Ryan KL, Frei MR, Dusch SJ, Lehnert HM, Kovatch RM. 2001. Repeated exposure of C3H/HeJ mice to ultra-wideband electromagnetic pulses: lack of effects on mammary tumors. Radiat Res. 155,369-377.
Javanmardi J, Stushnoff C, Locke E, Vivanco JM. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry 83, 547–550.
Kaya I, Yiğit N, Benli M. 2008. Antimicrobial activity of various extracts of ocimum basilicum and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr. J. Trad. CAM 5(4), 363 – 369.
Khaki A, Heidari M, Ghaffari Novin M, Khaki AA. 2008. Adverse effects of ciprofloxacin on testis apoptosis and sperm parameters in rats. Iran. J. Reprod. Med. 6(2), 71-76.
Khaki A, Fathiazad F, Nouri M, Khaki AA. 2011. Effect of Ocimum basilicum on apoptosis in testis of rats after exposure to electromagnetic field. African Journal of Pharmacy and Pharmacology 5(12), 1534-1537.
Khaki A, Imani SAM, Farhad S. 2012. Effects of rosmarinic acid on male sex hormones (testosterone-FSH-LH) and testis tissue apoptosis after exposure to electromagnetic field (EMF) in rats. African Journal of Pharmacy and Pharmacology 6, 248 – 252.
Kruma Z, Andjelkovic M, Verhe R, Kreicbergs. 2008. Phenolic compounds in basil,oregano and thyme. Coupure Links 653, B-9000, Ghent, Belgium.
Lis-Balchin M, Buchbauer G, Ribisch K, Wenger MT. 1998. Comparative antibacterial effects of novel Pelargonium essential oils and solvent extracts. Lett. Appl. Microbiol. 27, 135-141.
Meral I, Mert H, Nihat M, Deger Y, Yorukc I, Yetkind A, Keskine S. 2007. Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs. Brain Research 1169, 120 – 124.
Mezei G, Cher D, Kelsh M, Edinboro CH, Chapman P, Kavet R. 2005. Occupational Magnetic Field Exposure, Cardiovascular Disease Mortality and Potential Confounding by Smoking. AEP. 15(8), 622–629.
Niwano Y, Saito K, Yoshizaki F, Kohno M, Ozawa T. 2011. Extensive screening for herbal extracts with potent antioxidant properties. J Clin Biochem Nutr. 48, 1 – 83.
Regula KM, Kirshenbaum LA. 2005. Apoptosis of ventricular myocytes: a means to an end. J Mol Cell Cardiol. 38, 3–13.
Supawan B, Chanida P, Nijsiri R. 2007. Chemical compositions and antioxidative activities of essential oils from four Ocimum species endemic to Thailand J Health Res. 21(3), 201-6.
Roshangar B, Soleimani-Rad J, Ansaree R, Roshangar L. 2012. Effect of low frequency Electromagnetic Field on cardiovascular system: An ultrastructural and immunohistochemical study.Annals of Biological Research 3 (1), 81-87.
Sgherri C, Pinzino C, Navari-Izzo F, Izzo R. 2011. Contribution ofmajor lipophilic antioxidants to the antioxidant activity of basil extracts: an EPR study. J Sci Food Agric 91, 1128–1134.
Sharifian A. 2009. Effect of extremely low frequency magnetic field on antioxidant activity in plasma and red blood cells in spot welders Int Arch Occup Environ Health 82(2), 259-66.
Sharma M, Kishore K, Gupta SK, Joshi S, Arya DH. 2001. Cardioprotective potential of Ocimum sanctum in isoproterenol induced myocardial infarction in rats. Molecular and Cellular Biochemistry 225, 75–83.
Tada H, Murakami Y, Shimomura K, Ishimaru K. 1996. Rosmarinic acid and related phenolics in hairy root cultures of Ocimum basilicum. Phytochemistry 42, 431-434.
Wencker D, Chandra M, Nguyen K, Miao W, Garantziotis S, Factor SM, Shirani J, Armstrong RC, Kitsis RN. 2003. A mechanistic role for cardiac myocyte apoptosis in heart failure. J Clin Invest. 111, 1497 –1504.
Amir Afshin Khaki, Arash Khaki, 2012. Protection effects of basil on cardiac cells apoptosis after chronic exposure to electromagnetic field (EMF) in rats by TUNEL assay. Int. J. Biosci., 2(12), 13-20.
Copyright © 2012 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.