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Protection effects of basil on cardiac cells apoptosis after chronic exposure to electromagnetic field (EMF) in rats by TUNEL assay

Amir Afshin Khaki, Arash Khaki

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Int. J. Biosci.2(12), 13-20, December 2012


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With developing in electronic means and increase in using these concern about the public health hazards of chronic exposure to electromagnetic field has gained more attention Medicinal use of basil belong to ancient medicine in asia such as Iran. Wistar male rat (n=40) were allocated into four groups, control (n=10) and test groups (n=30), that subdivided into groups of 3, the extract group were received of basil extract (1.5g/kg body), second extract group were received of basil extract (1.5g/kg body) and emf group that exposed to50 Hz for 40 consequence day. In end of study the heart tissue of rats in whole groups were removed and prepared for pathology analysis by tunel method. Serum MDA, percentage of apoptotic cells and artery hyperemia significantly were increased in experimental group that has exposed to 50Hz EMF(p<0.05).the level of TAC in groups which received 1.5g/kg body basil extract significantly were increased (p<0.05) in comparison to control group. Morphology of heart in both experimental and control group were similar. Results revealed that administration of 1.5g/kg body of basil extract significantly decreased the apoptotic rate and protects cells by presents its antioxidant role.


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Protection effects of basil on cardiac cells apoptosis after chronic exposure to electromagnetic field (EMF) in rats by TUNEL assay

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