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Protective role of methanolic extract of medicinal plants in paracetamol induced toxicity in laying hens

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Research Paper 01/11/2019
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Protective role of methanolic extract of medicinal plants in paracetamol induced toxicity in laying hens

Muhammad Mushtaq, Naila Chand, Umer Saddique, Sarzamin Khan
Int. J. Biosci.15( 5), 527-539, November 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: Hepato-protectiveKidney histopathologyLiver histopathologyMedicinal plantsNephro protectiveParacetamol

Abstract

The study was designed to assess the hepato-nephro protective role of indigenous medicinal plants in paracetamol induced toxicity in layers. A total of 165 laying birds were reared and alienated into 11 groups with 3 replicates each. The group G-1 was considered as negative control, while G-II was positive control and the other groups were fed with different doses of medicinal plants methonolic extracts@ 1, 100 and 1000 mg/Kg b.w. Toxicity was induced through paracetamol @ 750 mg/Kg b.w. Toxicity was measured by evaluating the hepatic specific biomarkers (ALT, AST, ALP and serum Protein). Gross histopathology of liver and kidney was performed to evaluate the curative potential of medicinal plants against paracetamol toxicity. Results of this study revealed that liver biomarkers in the birds treated with paracetamol showed elevated trend (AST=51.66, ALT=40.00, ALP=4385 and serum protein 4.89 IU/L) indicating severe hepatic damage. However, in G-4 fed with @ 100 mg/kg b.w methanolic extract of Terminalia arjuna showed significant reduction in liver biomarkers (AST=18.00, ALT=15.00, ALP=2350.33 and serum protein 9.03 IU/L). This low trend of liver biomarkers indicating that plant extract help in restoring the normal functional ability of the hepatocytes. Histological section of liver and kidney in G-1 showed significant pathological changes included hydopic degeneration, fatty infiltration, hemorrhages and leukocytic infiltration in liver section. The renal section revealedmild glomerulo-tubular degeneration changes with mild leukocytic infiltration. However, in the birds treated with T. arjuna@ 100 mg/kg b.w positive response with maximum regenerative potential. It was concluded that the methanolic extract of Terminalia arjuna @ 100 mg/kg have a significant hepato-nephroprotective response against paracetamol induce toxicity.

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Abdouli H, Haj-Ayed M, Belhouane S, Emna EH. 2014. Effect of feeding hens with fenugreek seeds on Laying performance, egg quality characteristics, serum and egg yolk cholesterol. Journal of New Sciences 3(1). http://www.jnsciences.org

Abdul-Vahab A, Harindran DJ. 2016. Hepatoprotective activity of bark extracts of terminaliacatappalinn in albino rats. World Journal of Pharmacology and Pharmaceutical Science 5(6), 1002-16.

Adewale OB, Adekeye AO, Akintayo CO, Onikanni A. 2014. Carbon tetrachloride (CCl4)-induced hepatic damage in experimental Sprague dawley rats: Antioxidant potential of Xylopiaaethiopica. Journal of Psychopharmacology. 3, 118-123.

Adewale OB, Adekeye AO, Akintayo CO, Onikanni A. 2014. Carbon tetrachloride (CCl4)-induced hepatic damage in experimental Sprague dawley rats: Antioxidant potential of Xylopiaaethiopica. Journal of Psychopharmacology. 3, 118-123.

Ali R. 2014. Effect of Mint and Fenugreek and Mixture on Production and Immunity of Broilers. Journal ofKerbala University 12(2), 12-15.

Anusha M, Venkateswarlu M, Prabhakaran V, Taj SS, Kumari BP. 2011. Hepatoprotective activity of aqueous extract of Portulacaoleracea in combination with lycopene in rats. Indian Journal of Pharmacology 43, 563.

Bancroft JM, Gamble M. 2007. Theory and practices of histopathological techniques (5th Edition), Churchill Livingestone London. 125-138.

Blantz RC. 1996. Acetaminophen: acute and chronic effects on renal function. American Journal of Kidney Disease 28, S3–S6.

Chand N, Durrani FR, Ahmad S, Khan A. 2011. Immunomodulatory and hepatoprotective role of feed-added Berberis lycium in broiler chicks. Journal of Science Food and Agriculture 91(10), 1737-45.

Chaudhari GM, Mahajan TR. 2016. In Vitro Hepatoprotective Activity of Terminaliaarjuna Stem Bark and Its Flavonoids against CCl4 Induced Hepatotoxicity in Goat Liver Slice Culture. Asian Journal of Plant Sciences and Research 6(6), 10-17.

Criste RD, Panaite T, Bercaru A, Varzaru I, Ropota M, Cornescu MG. 2013. Study on the use of fenugreek in laying hens diets on egg quality, 19th European Symposium on Poultry Nutrition Potsdam, Germany.

Dabur R, Singh H, Chhillar AK, Ali M, Sharma GL. 2004. Antifungal potential of Indian medicinal plants. Fitoterapia journal 75(3-4), 389-391.

El-Nekeety AA, El-Kady AA, Soliman MS, Hassan NS, Abdel-WahhabM A. 2009. Protective effect of Aquilegia vulgaris (L.) against lead acetate-induced oxidative stress in rats. Journal of Food Chemistry and Toxicology 47(9), 2209–2215.

Ghazwan A, Raouf M, Vaibhav K, Khan A, Tabassum R, Ahmed MDE, Javed H, Chander K, Islam F, Siddiqui MS. 2013. Terminaliaarjuna bark extract inhibits histological alterations by mitigating oxidative stress in lead intoxicated mice. Oriental Pharmacology andExperimental Medicine. 13(4), 253–265.

Ghosh S, Gomes A. 2016.Hepatoprotective Efficacy of Gold Nanoparticle Synthesized by Green Method Using TrigonellaFoenum- Graecum Seed Extract. Translational Medicine (Sunnyvale) 6, 190. http://dx.doi.org/10.4172/2161-1025.1000190

Ghosh S, Sengupta J, Datta Pand Gomes A. 2014. Hematopoietic and antioxidant activities of gold nanoparticles synthesized by aqueous extract of fenugreek (Trigonellafoenum-graecum) seed. Advanced Science, Engineering and Medicine 6, 546-552.

Haidry MT, Malik A. 2014.Hepatoprotective and Antioxidative Effects of TerminaliaArjuna against Cadmium Provoked Toxicity in Albino Rats (RatusNorvigicus). Journal of Biochemistry and Pharmacology 3, 130. http://dx.doi.org/10.4172/2167-0501.1000130

Hebbani AV, Reddy VD, Varadacharyulu NCH. 2015. Protective Effect of Aqueous Bark Extract of Terminalia Arjuna against Alcohol-Induced Hepato and Nephrotoxicity in Rats. Int. Journal of Phytomedicine 7(2), 142-153.

Henderson R. 2016. Paracetamol (Acetaminophen) poisoning. https://patient.info/doctor/paracetamol-poisoning

Henrich WL. 1998. Analgesic nephropathy. Transaction of American Clinical and Climatological Association 109, 147– 158.

Jagadish NR, Mahmood R. 2008. Evaluation of Hepatoprotective activity of Echinops EchinatusRoxb Roots, Advances in Pharmacology and Toxicology. 9(2), 145-149.

Kumar P, Prasad R, Singh K, Kand Roy BK. 2009. Hepatoprotective effect of Centellaasiatica against paracetamol induced liver damage in broiler chicken. Indian Journal of PoultrySciences 44(1), 101-104.

Li J, Shi M, Ma B, Zheng Y, Niua R, Li K. 2017. Protective effects of fraction 4a of polysaccharides isolated from Lycium barbarum against KBrO3-induced renal damage in rats. Royal Socity of Chemisitry. Journal of Food Function 8, 2566-2572.

Lindenthal J, Sinclair JF, Howell S, Cargill L, Sinclair PR, Tailor T. 2003. Toxicity of paracetamol in cultured chick hepatocytes treated with methotrexate. European Journal of Pharmacology 228(5-6), 289-298.

Makwana M, Sharma A, Rathore HS. 2012. Shall herbs combat hepatorenal toxicity of paracetamol? Lambert Academic Publishing co. Germany.

Malik TA, Kamili AN, Chishti MZ, Ahad S, Tantry MA, Hussain PR, Johri RK. 2017. Breaking the resistance of Escherichia coli: Antimicrobial activity of Berberis lycium Royle. Journal of Microbial and Pathogen 102, 12-20.

Manna P, Sinha M, Sil PC. 2006. Aqueous extract of Terminaliaarjuna prevents carbon tetrachloride induced hepatic and renal disorders. BMC Complement Alternative Medicine 6, 33. http://dx.doi.org/10.1186/1472-6882-6-33.

Mo EF (Ministry of Environment and Forests).  2006. Proceedings of the International Conference on Vulture Conservation, New Dehli, Gov’t of India.

Moulisha B, Karan TK, Kar B, Haldar PK, Bhattacharya, Sanjib Ghosh K, Ashoke, Kumar, Suresh RB,  Haldar, Pallab. 2011. Hepatoprotective Activity of Terminaliaarjuna Leaf against Paracetamol-Induced Liver Damage in Rats. Asian Journal of Chemistry. 23.

Panaite T, Cornescu MG, Criste R. 2014. Effect of Fenugreek Supplements to High Fatty Acids Diets on Layer Performance. University of Agricultural Sciences and Veterinary Medicine Iasi.

Prasenjit M, Sinha M, Parames CS. 2006. Aqueous extract of Terminaliaarjuna prevents carbon tetrachloride induced hepatic and renal disorders. BMC Complement Alternative Medicine 6, 33.

Prescott LF, Speirs GC, Critchley JA, Temple RM, Winney RJ. 1989. Paracetamol disposition and metabolite kinetics in patients with chronic renal failure. European Journal of Clinical Pharmacology. 36, 291–297.

Ragavan B, Krishnakumari S. 2006.  Effect of T. arjunastem bark extract on histopathology of liver, kidney and pancreas of alloxan-induced diabetic rats. African Journal of Biomed and Research 9, 189–97.

Raj CD, Shabi MM, Jipnomon J, Dhevi R, Gayathri K, Subashini U, Rajamanickam GV. 2012. Terminaliaarjuna’s antioxidant effect in isolated perfused kidney. Journal of Research and Pharmacological Sciences 7(3), 181–188.

Ranganathan V, Punniamurthy D, Ahamad B, Kumar MS. 2013. Hepatoprotective activity of Indigoferaaspalathoides in chicken. Agriculture and Research Communication Center, Thanjavur (TN).

Shankreppa D, Desai, Patil BS, Kanthe PS, Potekar RM. 2015. Effect of ethanolic extract of terminaliaarjuna on liver functions andhistopathology of liver in albino rats fed with hyperlipidemic diet. International Journal of Pharmacology and Pharmaceutical Sciences 7(10), 302-306.

Sharma V, Singh P, Rani A. 2016. Antimicrobial activity of Trigonellafoenum-graecum L. (Fenugreek). European journal of Experimental Biology 7, 1.

Sharma A, Makwana M, Rathore HS. 2008. Can herbal paracetamol combination drug prevent both liver and kidney disease?. Results and possibilities. Ethanobotonical Leaflets, USA, 12, 286-298.

Shihabudeen MH, Priscilla DH, Thirumurugan K. 2010. Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal plants. International Journal of Pharmaceutical Sciences and Research 1, 430-434.

Shirish SP. 2011. Protective effect of Terminalia Arjuna against CCl4 induced liver damage in rats. Scholars Research Library Der Pharmacia Lettre 3(1), 84-90.

Sivalokanathan S, Ilayaraja M, Balasubramanian MP. 2006. Antioxidant activity of Terminaliaarjuna bark extract on N-nitrosodiethylamine induced hepatocellular carcinoma in rats.Molecular and Cell Biochemistry. 281(1-2), 87-93.

Steel RGD, Torrie JH. 1981. Principles and procedures of statistics: A biometrical approach. 2nd. Ed. McGraw-Hill, Singapore.

Sun FY, Chen X P, Wang JH, Qin HL, Yang SR, Du GH. 2008. Arjunic acid, a strong free radical scavenger from Terminaliaarjuna. American Journal of Chinese Medicine 36, 197-207.

Swan G, Naidoo V, Cuthbert R, Green RE, Pain DJ, Swarup D. 2006. Removing the threat of diclofenac to critically endangered Asian Vulture. Public Library of Science Biology 4, 66.

Trumper L, Monasterolo LA, Elias MM. 1998. Probenecid protects against in vivo acetaminophen-induced nephrotoxicity in male Wistar rats. Journal of Pharmacology and Experimental Therapeutics. 284, 606–610.

Vikas K, Sharma N, Prem AS, Deva KKK. 2018. Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminaliaarjunafrom north-western Himalayas, Indian Journal of Traditional and Complementary Medicine 8(1), 100-106.

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