Protein profiles and cross antigenicity analysis for outer surface proteins of two streptococcus species, isolated from hybrid red tilapia (Oreochromis spp.)

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Research Paper 01/08/2020
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Protein profiles and cross antigenicity analysis for outer surface proteins of two streptococcus species, isolated from hybrid red tilapia (Oreochromis spp.)

Muhammad Rahmatullah, Hassan Mohd Daud, Sabri Mohd Yusoff, MY Ina-Salwany
Int. J. Biosci.17( 2), 256-267, August 2020.
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Streptococcosis is one of the most devastating diseases for hybrid red tilapia (Oreochromis spp.). Streptococcus iniae and Streptococcus agalactiae are considered as the most prominent etiological agents that cause streptococcosis in fish, consequently resultant high mortality and contribute big losses towards the farmer’s economy. Outer surface proteins (OSPs) play an important role to induce protective immunity against pathogens. To study antigenicity of S. iniae and S. agalactiae proteins, OSPs of these two bacteria were extracted by sodium lauryl-sarcosinate method and analyzed by SDS PAGE. Protein profiling revealed 40 and 52 kDa for S. iniae, while only 52 kDa was observed for S. agalactiae as major proteins. Our results in Western blotting demonstrated that rabbit anti-serum of S. iniae could able to detect the antigenic proteins for S. iniae and S. agalactiae in both of the homologous and heterologous reactions. Therefore, antigenic gene encoding 52 kDa OSP of S. iniae was PCR-amplified and successfully cloned in pET32Ek/LIC vector. The cloned sequence was identified as 1305 bp nucleotide sequence of sip gene coding for polypeptides of 434 amino acid residues with 99% similarity to other surface immunogenic gene (sip) of S. agalactiae in GenBank. Based on predicted 20 antigenic sites, it can be assumed that sip gene of S. iniae should play a great role in bacterial antigenicity which can be used as an efficient streptococcal vaccine development for tilapia farming.


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