Paper Details

Proteomic analysis of salt -responsive proteins in canola leaves

Mahmoud Toorchi, Maryam Kholgi

DOI: https://dx.doi.org/10.12692/ijb/5.10.68-76

Int. J. Biosci. 5(10), 68-76. November, 2014. (PDF)

Abstract:

Salinity is one of the major abiotic stresses limit agricultural productivity worldwide. To identify the mechanisms of salt responsiveness in canola, the proteinsexpressed  in the leaves of salt-tolerant, Hyola 308 was analyzed. Plants were exposed to 0, 150, and 300mM NaClduring the vegetative stage. An increase in the Na content and a reduction in K contend of shoot and root was observed.  K/Na discriminant ratios were significantly reduced in leaves and roots due to salt stress.Two-dimensional polyacrylamide gel electrophoresis coupled with mass spectrometry analysis could identifyfourteen salt responsive proteins by Coomassie brilliant blue staining. These proteins functionally involved in oxidative stress, photosynthesis, signal transduction, protein kinase, transcription and proteases.These proteins might control the sensitivity of several regulatory genes to short exposure of canola to salt stress.