Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 1, 2016

| Download 1

Protocol on direct organogenesis in Chrysanthemum morifolium Ramat.

Rawnak Jahan Swarna, Dilruba Yeasmin, Md. Mostafizur Rahman, Md. Firoz Alam

Key Words:

Int. J. Biosci.9(4), 123-137, October 2016

DOI: http://dx.doi.org/10.12692/ijb/9.4.123-137


IJB 2016 [Generate Certificate]


An efficient direct organogenesis protocol was developed for Chrysanthemum morifolium Ramat., an ornamental herb. The purpose of this study was to determine the effect of different growth regulators on shoot formation and multiplication over the cultural period and mass propagation in large quantities in a short time using tissue culture techniques. Nodal segments, after being sterilized with 0.1% HgCl2 (mercuric chloride) for eight minutes, were inoculated in MS media (Murashige and Skoog, 1962) with different concentrations of BAP (6-benzyl amino purine) singly and combinations with KIN (6-furfural amino purine), Casein hydrolysate (CH), NAA (α-napthalene acetic acid). All of that, shoot growth and development showed better performance in 1.0 mg/l BAP singly, and 2.0 mg/l BAP with 0.5 mg/l KIN. In vitro rooting was successfully achieved on MS media supplemented with different concentrations of auxins. Better rooting response was obtained in MS media supplemented with 2.0 mg/l and 2.5 mg/l IBA (Indole-3 butyric acid) and 0.5 mg/l IBA with 1.5 mg/l NAA, respectively. The results from the study will enhance rapid multiplication of high quality planting materials of Chrysanthemum morifolium and other valuable plants.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Protocol on direct organogenesis in Chrysanthemum morifolium Ramat.

Akhter N. 1998. In vitro culture and plant regeneration of Chrysanthemum morifolium Ramat. M.Sc, Thesis, Department of Botany, University of Rajshahi.

Ali A, Afrasiab H, Naz S, Rauf M, Iqbal J. 2008. An efficient protocol for in vitro propagation of Carnation (Dianthus caryophyllus).Pakistan Journal of Botany 40(1), 111-121.

Amin MN,  Karim  MZ,  Asaduzzaman, Islam S, Hossain F, Alam R. 2002. Rapid multiplication of Chrysanthemum morifolium through in vitro culture. Pakistan Journal of Biological Sciences 5(11), 1170-1172.

Awal SMA, Alam MJ, Ali MR, Hasan MNU. 2005. In vitro Propagation of Pointed Gourd (Trichosanthes dioica Roxb.) from Shoot Tips. Biotechnology 4(3), 221-224.

Ben-Jaacov J, Langhans RW. 1972. Rapid multiplication of chrysanthemum plants by stem-tip proliferation. Horticultural Science 7(3), 289-290.

Bhattacharya P, Dey S, Das N, Bhattacharya BC. 1990. Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Reports 9(8), 439-442.

Chae SC. 2014. Influence of media on in vitro root regeneration and micropropagation of Chrysanthemum morifolium Ramat cv. Hwiparam. Life Science Journal 11(9), 797-799.

Chakrabarty D, Mandal AKA, Datta SK.  2000.  SFM and light microscopic studies on direct shoot regeneration from ray florets of Chrysanthemum. Israel Journalof Plant Sciences 48(2), 105-107.

Choudhary ML. 1991. Effect of medium components and explants position on in vitro shoot proliferation of Indian rose. Natl. Acad. Lett. 14, 11-11.

Choudhary ML. 1991. Vegetative propagation of carnation in vitro through multiple shoot development. Indian Journal of Horticulture 4(2), 177-181.

Das B, Haque MM, Islam AS, Rahman MH, Hoque MI. 1986. In vitro plantlet development in Jute. Prop. Nayudamma Mem. Sym.On Agricultural Application on Biotechnology. Madras, India. 106-114 p.

Din MSU, Nasir UJK, Zaman S, Reza MA. 2005. Regeneration of Multiple Shoots from Different Explants viz. Shoot Tip, Nodal Segment and Cotyledonary Node of in vitro Grown Seedlings of Peltophorumm pterocarpum (DC.) Backer ex K. Heyne. Biotechnology 4(1), 35-38.

Donato Mde, Perucco E. 1984. Micropropagation of Chrysanthemum by means of lateral meristem stimulation.Annali-della-Facolta-di-Scienze-Agarie-della-universita-degli-st-udi-di-Torino. 13, 103-115.

Earle EP, Langhans RW. 1973. Propagation of Chrysanthemum in vitro. 1. Multiple plantlets from shoot tips and the establishment of tissue culture. Journal of the American Society for Horticultural Science 9, 128-132.

Fatema M. 1995. Micropropagation of Chrysanthemum morifolium Ramat through tissue culture.M.Sc. Thesis, Department of Botany, University of Dhaka.

Gautheret RJ. 1939. Sur Ia possibility de realiserIa culture indefinie des tissue de tubercules de carrotle. C. R.Hebd, Seanc. Comptes Rendus de l’Académie des Sciences 208, 118-121.

Gul A. 2001. Microprpagation of Chrysanthemum. M.Sc. thesis. Department of Botany, University of Peshawar.

Haberlandt G. 1902. Kulturversuche mit isolierten pflanzenzellen. Sitzungsber. Akad.Wiss. Wien. Math-Naturwiss. Kl. Abt. J. 111, 69-92.

Hahn EJ, Boe JH, Lee YB. 1998. Growth and leaf surface characteristics of Chrysanthemum plantlets in micropropagation and microponic system. Journal of Korean Society for Horticultural Science 39, 838-842.

Hodson DJE, Ferero A, Cancina G, Morena AM, Monslave LE, Acero W.  2008. In vitro regeneration of three Chrysanthemum (D. Grandiflora) varieties Via. organogensis and somatic embryogenesis. Univ Sci. 13(2), 118-127.

Hoque MI, Jahan MT, Sarkar RH. 1998. In vitro Shoot Regeneration and Ex vitro Rooting in Chrysanthemum morifolium Ramat. Plant Tissue Culture 8(1), 157-164.

Hoque MI. 1995. In vitro multiple shoot regeneration in Chrysanthemum morifolium Ramat. Plant Tissue Culture 5(2), 153-162.

Jaime A, Teixeiria SD. 2004. Ornamental Chrysanthemums: Improvement by biotechnology. Plant Cell Tissue and Organ Culture. 79, 1-8.

Jha N. 2016. Micro propagation: Technique, Factors, Applications and Disadvantages. http://www.biologydiscussion.com/biotechnology/clonal-propagation/micro-propagation-technique factors-applications-and-disadvantages/10732.

Karim MZ, Amin MN, Azad MAK, Begum F , Rahman MM, Ahmad S, Alam R. 2003. In vitro Shoot Multiplication of Chrysanthemum morifolium as Affected by Sucrose, Agar and pH. Biotechnology 2, 115-120.

Karim MZ, Amin MN, Azad MAK, Begum F, Islam MM, Alam R.  2002. Effect of different Plant Growth regulators on in vitro Shoot Multiplication of Chrysanthemum morifolium. Online Journal of Biological Science 3(6), 553-560.

Kaul V, Miller RM, Hutchinson JF, Richards D. 1990. Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev (Syn. Chrysanthemum morifolium Ramat.). Plant Cell Tissue and Organ Culture 21, 21-30.

Khalid N, Davey MR, Power JB. 1989. The generation of plants of potential commercial value. Scientia Horticulture 38(3-4), 287-294.

Khan MA, Khanam D, Ara AK, Hossain AKMA. 1994. In vitro Plant regeneration in Chrysanthemum morifolium Ramat. Plant Tissue Culture 4(1), 53-57.

Komalavalli N, Rao MV. 2000. In vitro micro-propagation of Gymnemam slyvestre. A multipurpose medicinal plant. Plant Cell Tissue and Organ Culture 61, 97-105.

Kumari M, Varghese TM. 2003. Effect of different growth regulators on fresh and dry weight of callus and regeneration in chrysanthemum cultivars Miss Universe and Snow Ball. Journal of Ornamental Horticulture 6(3), 188-194.

Litz RE, Jaiswal VS. 1990. Micropropagation of tropical and sub-tropical fruits. In: Debergh and Zimmerman RH (eds). Kluwer Academic Publishers, Dordrecht.Micropropagation Technol. Application.  247-266 P.

Lu CY, Nugent G, Wardley T. 1990. Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple). Plant Cell Reports 8(12), 733-6.

Misra P, Datta SK. 2007. Standardization of in vitro protocol in Chrysanthemum cv. Madam E Roger for development of quality planting material and to induce genetic variability using r- radiations. Indian Journal of Biotechnology 16, 121-124.

Moss JP, Dutt NRG, Lingamaneni A. 1988. Root induction of in vitro grown shoots of Arachis species and a hybrid. IAN. 4, 25-26.

Munshi MK, Hakim L, Islam MR, Ahmed G. 2004. In vitro clonal propagation of Banyan (Ficus benghalensis L.) through axillary bud culture. International Journal of Agriculture and Biology 6(2), 321-323.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497.

Nahid JS, Shyamali S, Kazumi H. 2007.  High frequency shoot regeneration from petal explants of Chrysanthemum morifolium In vitro. Pakistan Journal of Biological Sciences 10(19), 3356-3361.

Pant M, Lal A, Jain R. 2015. A simple cost effective method for mass propagation of Chrysanthemum morifolium. JAPS. 5(07), 103-111.

Prasad RN, Chaturvedi HC. 1988. Effect of season of collection of explants on micropropagation of Chrysanthemum morifolium. Biologia Plantarum 30(1), 20-24.

Radojevic LS, Jevremovic, Lazic B. 2000. Stem segment In vitro culture of Chrysanthemum as a method for micropropagation. Glasnik-Sumarskog-Fakulteta-Univerzitet-u-Beogradu. 83, 111-118.

Rajani H, Patil SS. 2009. In vitro response of different explants’ types on shoot and root development of Ginger. ISHS Acta Horticulturae 829: VI International Symposium on In Vitro Culture and Horticultural Breeding.

Roset S, Bokelman GS. 1975. Vegetative propagation of Chrysanthemum morifolium Ramat. In vitro Scientia Horticulturae 3, 317-330.

Rout GR, Das P. 1997. Recent trends in the biotechnology of Chrysanthemum – A Critical Review. Scientia Horticulturae, 69(3-4), 239-257.

Sarker RH, Shaheen I. 2001. In vitro Propagation of Chrysanthemum (Chrysanthemum morifolium Ramat.) Through Callus Culture. Plant Tissue Culture 11(1), 85-91.

Shanti RP, Balachandran S, Anitha C, Chopde M. 2005. In vitro propagation of chrysanthemum.J. Soil and Crops. 15(2), 287-289.

Shatnawi M, Al-Fauri A, Megdadi H, Kair M, Al-Shatnawi, Saead RS, Abu-Romman, Al-Ghzawi AL. 2010. In Vitro Multiplication of Chrysanthemum morifolium Ramat. and it is Responses to NaCl Induced Salinity. Jordan Journal of Biological Sciences 3(3), 101-110.

Teixeira da Silva JA. 2003. Tissue culture and cryo-preservation of chrysanthemum: a review. Biotechnology Advances 21, 715-766.

Trifunovic M, Jevremovic S, Nikolic M, Subotic A, Radojevic LJ. 2006.Micropropagation of Chrysanthemum cultivars-effect of cold storage on plant regeneration In vitro. XXVII International Horticultural Congress IHC2006. International Symposium on Plant Biotechnology. ISHS Acta Horticulturae-764.

Tripepi RR. 1997. Adventitious shoot regeneration. In: (Eds.): R.L.Geneve, J.E. Preece and S.A. Merkle. Biotechnology of Ornamental Plants, Biotechnology in Agriculture Series, No 16. CAB International, Wallingford, U.K. 45-71 P.

Vantu S. 2006. Organogenesis in Chrysanthemum morifolium Ramat. (Cultivar “Romica”) Callus Cultures. Analelestiintifice ale Universitatii “Al. l. Cuza” lasiTomul LII, s. ll a. Biologievegetala. Faculty of Biology. 71 P.

Wang K, Mao Y, Zhang X. 2000. A preliminary study on tissue culture of stem apex of Chrysanthemum morifolium. Zhong Yao Cai. 23(3), 125-7.

Waseem K, Jilani MS, Jaskani MJ, Khan MS, Kiran M, Khan GU. 2011. Significance of different plant growth regulators on the regeneration of Chrysanthemum plantlets (Dendranthema morifolium L.) through shoot tip culture. Pakistan Journal of Botany 43(4), 1843-1848.

Waseem K, Jilani MS, Khan MS, Kiran M, Khan GU. 2011. Efficient in vitro regeneration of Chrysanthemum (Chrysanthemum morifolium L.) plantlets from nodal segments. African Journal of Biotechnology 10(8), 1477-1484.

Waseem K, Jilani MS, Khan MS. 2009. Rapid plant regeneration of chrysanthemum (Chrysanthemum morifolium L.) through shoot tip culture. African Journal of Biotechnology 8, 9.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background