Qualitative analysis of phytoconstituents from Azima tetracantha L. and evaluation of its antimicrobial activity
Paper Details
Qualitative analysis of phytoconstituents from Azima tetracantha L. and evaluation of its antimicrobial activity
Abstract
Azima tetracantha L., a medicinal plant from the Salvadoraceae family, is traditionally recognized for its diverse therapeutic properties, including antimicrobial, anti-inflammatory, hepatoprotective, and hypoglycemic effects. This study aimed to screen the phytochemical constituents and evaluate the antimicrobial potential of its leaf extracts. The phytochemical analysis revealed the presence of key bioactive compounds such as tannins, flavonoids, phenolic compounds, lignin, and terpenoids in ethyl acetate and ethanol extracts. The antimicrobial activity was assessed using the disc diffusion method against various bacterial (Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Salmonella typhi, and Staphylococcus epidermidis) and fungal (Aspergillus niger, Aspergillus flavus, and Candida sp.) strains. The ethanol extract demonstrated the highest inhibition against Escherichia coli (11 mm), Streptococcus mutans (11 mm), and Staphylococcus epidermidis (12 mm). The ethyl acetate extract exhibited notable inhibition against Escherichia coli (8 mm) and Staphylococcus epidermidis (6 mm). The diethyl ether extract showed strong inhibition against Escherichia coli (12 mm) but was less effective against other bacterial strains. Petroleum benzene extract exhibited limited activity, with inhibition observed only against Streptococcus mutans (7 mm). Mixed solvent extracts displayed the highest inhibition against Escherichia coli (20 mm), Streptococcus mutans (20 mm), Staphylococcus epidermidis (15 mm), and Candida sp. (16–20 mm). GC-MS analysis identified several bioactive metabolites, further highlighting the pharmaceutical potential of Azima tetracantha L. The findings support its traditional medicinal use and suggest its potential role in developing novel antimicrobial agents.
Begum TN, Muhammad Ilyas MH, Burkanudeen A, Kalavathy S, Vijaya Anand A, Sampathkumar P, Jaswanth. 2009. Hypoglycemic and antihyperlipidemic activity of ethanolic leaf extract of Azima tetracantha Lam. on alloxan-induced diabetic rats. J. Cell Tissue Res. 9(1), 1681–1685.
Bennett RN, Mellon FA, Rosa EA, Perkins L, Kroon PA. 2004. Profiling glucosinolates, flavonoids, alkaloids, and other secondary metabolites in tissues of Azima tetracantha Lam. (Salvadoraceae). J. Agric. Food Chem. 52(19), 5856–5862.
Bennett RN, Rosa EA, Perkins L, Kroon PA. 2004. Profiling glucosinolates, flavonoids, alkaloids, and other secondary metabolites in tissues of Azima tetracantha L. (Salvadoraceae). J. Agric. Food Chem. 52(19), 5856–5862.
Kekuda PT, Raghavendra HL. 2017. Phytochemistry, traditional uses, and pharmacological activities of Azima tetracantha Lam. (Salvadoraceae) – An updated review. International Journal of Green Pharmacy 10, 217–229.
Mottaleb MA, Sarker SD. 2012. Accelerated solvent extraction for natural products isolation. Methods in Molecular Biology 864, 75–87.
Nandagude TD, Bhojwani AP, Krishna Kinage. 2007. Analgesic activity of various extracts of leaves of Azima tetracantha Lam. Int. J. Green Pharm. 29, 37–38.
Nandgude TD, Bhojwani AP, Krishna Kinage. 2007. Analgesic activity of various extracts of leaves of Azima tetracantha Lam. Int. J. Green Pharm. 1(1), 37–38.
Oman M, Skerget M, Knez Z. 2013. Application of supercritical fluid extraction for the separation of nutraceuticals and other phytochemicals from the plant material. Macedonian Journal of Chemistry and Chemical Engineering 32(2), 183–226.
Prabu K, Samydurai P, Subbaiyan B, Thangapandian V. 2013. Phytochemical constituents and gas chromatography-mass spectrometry analysis of Caralluma diffusa (weight) N.E.BR. Aerial part. International Journal of Pharmacy and Pharmaceutical Sciences 5(3), 602–605.
Raaman N. 2006. Phytochemical Techniques. 1st ed. New India Publishing Agency, New Delhi, p. 19–25.
Rall GJH, Smalberger TM, De Waal HL, Arndt RR. 1967. Dimeric piperidine alkaloids from Azima tetracantha. Tetrahedron Lett. 3465–3469.
Rao VE, Prasada Rao PRS. 1978. Occurrence of triterpenoids in Azima tetracantha. Curr. Sci. 47, 857.
Saxena M, Saxena J, Nema R, Singh D, Gupta A. 2013. Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry 1(6), 168–182.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. 2011. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia 1(1), 98–106.
Williams UV, Nagarajan S. 1988. Isorhamnetin-3-O-rutinoside from leaves of Azima tetracantha Lam. Ind. J. Chem. 27, 387.
J. Thaha Thariq, L. Prince (2025), Qualitative analysis of phytoconstituents from Azima tetracantha L. and evaluation of its antimicrobial activity; IJB, V26, N1, January, P156-162
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