Quantification of betulinic, oleanolic and ursolic acid as medicinally important triterpenoids in wild and in vitro callus culture of Salvia sahendica (Lamiaceae): a comparative study
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Quantification of betulinic, oleanolic and ursolic acid as medicinally important triterpenoids in wild and in vitro callus culture of Salvia sahendica (Lamiaceae): a comparative study
Abstract
In the present study, quantitative determination of betulinic acid (BA), oleanolic acid (OA) and ursolic acid (UA) as well-known medicinal pentacyclic triterpenoids was simultaneously carried out in wild and in vitro callus culture of Salvia sahendica Boiss. Buhse (Lamiaceae) by reverse-phase high performance liquid chromatography (HPLC). The plant is an endemic medicinal species which is growing in the northwest of Iran. Callus induction (100%) was achieved from young leaf and intermodal explants cultured on Murashige and Skoog (MS) supplemented with 1.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L 6-benzylaminopurine (BAP). Our results revealed that the content of BA, OA and UA in the aerial parts of wild S. sahendica was 15.05, 645.93 and 112.92 mg per 100g dry weight (DW) (standard deviation: 0.73-3.0), respectively while their content in callus culture of the plant were 17.28, 126.27 and 121.59 mg per 100g DW (standard deviation: 1.6-3.2), respectively. Our findings show the merit of in vitro callus culture of S. sahendica for production of medicinally important triterpenoids. It can be also provide an ample opportunity to take this plant for extensive research for mass cultivation on plants and enhanced the production of these compounds through different biotechnological strategies like cell suspension cultures and large scale cultivation in bioreactor system.
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