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Quantitative gene expression analysis of NHX1 and HvPIP2;1 in barley (HordeumvulgareL.) under salinity stress

Sara Ghaffarian, Seyyed Abolghasem Mohammadi, Mahmoud Toorchi, Yadollah Omidi

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J. Bio. Env. Sci.7(3), 207-219, September 2015


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Plant sodium transporters activity and plasma membrane intrinsic proteins are the most important salt tolerance mechanisms in plants. In the present study, the expression pattern of genes encoding Na+/H+ exchanger (NHX1) and a plasma membrane intrinsic protein (HvPIP2;1) was investigated in three barley genotypes (Sahara3771 and an Iranian advanced line as salt tolerance and Clipper as salt susceptible) by the quantitative Real-time-PCR. The plants were exposed to 0, 100 and 200 mM NaCl at the seedling stage and root samples were harvested 24 hour, 3 days and 3 weeks after salt treatment. The results indicated that root length, fresh and dry weight were decreased by increase of salt concentration and duration. In response to 200 mM NaCl, mRNA level of NHX1gene showed slight increase in Sahara3771 and about 7-fold increase in advanced line, whereas there was no changes in Clipper compare with control. In all three genotype, expression of HvPIP2;1 decreased during the 24 h of after salt treatment, but increased thereafter. In general, the mRNA levels of the studied genes in Sahara3771 and advanced line as salt tolerant genotypes were higher than Clipper (salt susceptible). Suggesting, this may be related to their greater ability to sequester Na+ into sub-cellular compartments and/or maintain K+ homeostasis and better water adjustment ability.


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Quantitative gene expression analysis of NHX1 and HvPIP2;1 in barley (HordeumvulgareL.) under salinity stress

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