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Quantitative gene expression analysis of NHX1 and HvPIP2;1 in barley (HordeumvulgareL.) under salinity stress

Sara Ghaffarian, Seyyed Abolghasem Mohammadi, Mahmoud Toorchi, Yadollah Omidi

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J. Bio. Env. Sci.7(3), 207-219, September 2015

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Abstract

Plant sodium transporters activity and plasma membrane intrinsic proteins are the most important salt tolerance mechanisms in plants. In the present study, the expression pattern of genes encoding Na+/H+ exchanger (NHX1) and a plasma membrane intrinsic protein (HvPIP2;1) was investigated in three barley genotypes (Sahara3771 and an Iranian advanced line as salt tolerance and Clipper as salt susceptible) by the quantitative Real-time-PCR. The plants were exposed to 0, 100 and 200 mM NaCl at the seedling stage and root samples were harvested 24 hour, 3 days and 3 weeks after salt treatment. The results indicated that root length, fresh and dry weight were decreased by increase of salt concentration and duration. In response to 200 mM NaCl, mRNA level of NHX1gene showed slight increase in Sahara3771 and about 7-fold increase in advanced line, whereas there was no changes in Clipper compare with control. In all three genotype, expression of HvPIP2;1 decreased during the 24 h of after salt treatment, but increased thereafter. In general, the mRNA levels of the studied genes in Sahara3771 and advanced line as salt tolerant genotypes were higher than Clipper (salt susceptible). Suggesting, this may be related to their greater ability to sequester Na+ into sub-cellular compartments and/or maintain K+ homeostasis and better water adjustment ability.

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Quantitative gene expression analysis of NHX1 and HvPIP2;1 in barley (HordeumvulgareL.) under salinity stress

Aharon R, Shahak Y, Rozalina S, Wininger B, Kapulnik Y, Galili G. 2003. Over expression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress, Plant Cell 15, 439–447.

Alexandersson E, Fraysse L, Sjo¨vall-Larsen S, Gustavsson S, Fellert M, Karlsson M, Johanson U, Kjellbom P. 2005. Whole gene family expression and drought stress regulation of aquaporins. Plant Molecular Biology 59, 469–484.

Bienert GP, Thorsen M, Schussler MD, Nilsson HR, Wagner A, Tamas MJ, Jahn TP. 2008. A subgroup of plant aquaporins facilitate the bi-directional diffusion of As (OH)3 and Sb(OH)3 across membranes, BMC Biology 6, 1-15.

Blumwald E, Aharon GS, Apse MP. 2000. Sodium transport in plant cells. Biochimicaet Biophysica Acta 1465, 140-151.

Brini F, Hanin M, Mezghani I, Berkowitz G, Masmoudi K. 2007. Overexpression of wheat Na+/H+antiporter TNHX1 and H+-pyrophosphatase TVP1 improve salt- and drought-stress tolerance in Arabidopsis thaliana plants. Journal of Experimental Botany 58, 301–308.

Chaumont F, Tyerman SD. 2014. Aquaporins: Highly Regulated Channels Controlling Plant Water Relations. Plant Physiology 164, 1600–1618.

FAO. 2012. Faostat. FAO, Rome. www.faostat.fao.org.

Flowers TJ, Garcia A, Koyama M, Yeo AR. 1997. Breeding for salt tolerance in crop plants, the role of molecular biology. Acta Physiologiae Plantarum 19, 427-433.

Fujiyoshi Y, Mitsuoka K, de Groot BL, Philippsen A, Grubmuller H, Agre P, Engel A. 2002. Structure and function of water channels. Current Opinionin Structural Biology 12, 509–515.

Fukuda A, Chiba K, Maeda M, Nakamura A, Maeshima M, Tanaka Y. 2004. Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+pyrophosphatase, H+-ATPase subunit A, and Na+/H+antiporter from barley. Journal of Experimental Botany 55, 585-94.

Garbarino J, Dupont FM. 1989. Rapid induction of Na+/H+ exchange activity in barley root tonoplast. Plant Physiology 89, 1-4.

Greenway H. 1965. Plant response to saline substrates. Growth and ion uptake throughout plant development in two varieties of Hordeumvulgare. Australian Journal of Biological Science18, 763–779.

Heymann JB, Engel A. 1999. Aquaporins: phylogeny, structure, and physiology of water channels. News in PhysiologicalSciences14, 187–193.

Horie T, Kaneko T, Sugimoto G, Sasano S, Panda SK, Shibasaka M, Katsuhara M. 2011. Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots. Plant and Cell Physiology 52, 663–675.

Kaldenhoff R, Grote K, Zhu JJ, Zimmermann U. 1998. Significance of plasmalemmaaquaporins for water-transport in Arabidopsis thaliana. Plant Journal 14, 121–128.

Katsuhara M, Akiyama Y, Koshio K, Shibasaka M, Kasamo K. 2002. Functional analysis of water channels in barley roots, Plant and Cell Physiology 43, 885–893.

Katsuhara M, Koshio K, Shibasaka M, Hayashi H, Hayakawa T, Kasamo K. 2003. Over-expression of a Barley Aquaporin Increased the Shoot/ Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants, Plant and Cell Physiology 44, 1378–1383.

Leonova TG, Goncharova EA, Khodorenko AV, Babakov AV. 2005. Characteristics of salt-tolerant and salt-susceptible cultivars of barley. Russian Journal of Plant Physiology 52, 774–778.

Lian HL, Yu X, Ye Q, Ding XS, Kitagawa Y, Kwak SS, Su WA, Tang ZC. 2004. The role of aquaporin RWC3 in drought avoidance in rice. Plant and Cell Physiology 45, 481-489.

Ligaba A, Katsuhara M. 2010. Insights into the salt tolerance mechanism in barley (Hordeumvulgare) from comparisons of cultivars that differ in salt sensitivity. Journal of Plant Research 123, 105–118.

Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods 25, 402-408.

Maathuis FJM, Amtmann A. 1999. K+ nutrition and Na+ toxicity: the basis of cellular K+/Na+ ratios. Annals of Botany 84, 123-33.

Mahajan S, Tuteja N. 2005. Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics 444, 139-58.

Maurel C, Chrispeels MJ. 2001. Aquaporins: a molecular entry into plant water relations. Plant Physiology1251, 135–138.

Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Physiology 59, 651-681.

Munns R. 2001. Avenues for increasing salt tolerance of crops. In: Horst J et al. (eds) Plant nutrition: food security and sustainability of agro-ecosystems. Kluwer Dordrecht, 370–371.

Pardo JM, Cubero B, Leidi EO, Quintero FJ. 2006. Alcalication exchangers: roles in cellular homeostasis and stress tolerance. Journal of Experimental Botany57, 1181–1199.

Qiu QS, Guo Y, Quintero FJ, Pardo JM, Schumaker KS, Zhu JK. 2004. Regulation of vacuolar Na+/H+ exchange in Arabidopsis thaliana by the Salt-Overly-Sensitive (SOS) pathway. The Journal of Biological Chemistry 279, 207–215.

Rezaei Mashaeia M, Nematzadeha GA, Askarib H, Mozaffari Nejadc AS, pakdind A. 2014. Quantitative gene expression analysis of some sodium ion transporters under salinity stress in Aeluropuslittoralis. Saudi Journal of Biological Science 21, 394–399.

Rodriguez-Rosales MP, Galvez FJ, Huertas R, Aranda MN, Baghour M, Cagnac O, Venema K. 2009. Plant NHX cation/proton antiporters. Plant Signaling and Behavior 4, 265– 276.

Royo A, Aragüe´s R, Playa´n E, Ortiz R. 2000. Salinity-grain yield response functions of barley cultivars assessed with a drip injection irrigation system. Soil Science Society of America Journal 64, 359–365.

Royo A, Aragüe´s R. 1999. Salinity-yield response functions of barley genotypes assessed with a triple line source sprinkler system. Plant and Soil 209, 9– 20.

Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M. 2005. Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant and Cell Physiology 46, 1568–1577.

Tan WK, Lin Q, Lim TM, Kumar P, Loh CS. 2013. Dynamic secretion changes in the salt glands of the mangrove treespecies Avicenniaofficinalis in response to a changing saline environment. Plant, Cell and Environment36, 1410-1422.

Tester M, Davenport R. 2003. Na+ tolerance and Na+ transport in higher plants. Annals of Botany91, 503–527.

Wu CA, Yang GD, Meng QW, Zheng CC. 2004. The cotton GhNHX1 gene encoding a novel putative tonoplast Na+/H+antiporter plays an important role in salt stress. Plant, Cell and Physiology 45, 600-607.

WidodoPJH, Patterson JH, Newbigin E, Tester M, Bacic A, Roessner U. 2009. Metabolic responses to salt stress of barley (Hordeumvulgare L.) cultivars, Sahara and Clipper, which differ in salinity tolerance. Journal of Experimental Botany 60, 4089-4103.

Xu HX, Jiang XY, Zhan KH, Cheng XY, Chen XJ, Pardo JM, Cui D. 2008. Functional characterization of a wheat plasma membrane Na+/H+antiporter in yeast. Archives of Biochemistry and Biophysics 473, 8–15.

Xue ZY, Zhi DY, Xue GP, Zhang H, Zhao YX, Xia GM. 2004. Enhanced salt tolerance of transgenic wheat (Triticumaestivum L.) expressing a vacuolar Na+/H+antiporter gene with improved grain yields in saline soils in the field and a reduced level of leaf Na+. Plant Science167, 849–859.

Yang Q, Chen ZZ, Zhou XF, Yin HB, Li X, Xin XF, Hong XH, Zhu JK, Gong Z. 2009. Overexpression of SOS (Salt Overly sensitive) genes increases salt tolerance in transgenic Arabidopsis. Molecular Plant 2, 22-31.

Yokoi S, Quintero FJ, Cubero B, Ruiz MT, Bressan RA, Hasegawa PM, Pardo JM. 2002. Differential expression and function of Arabidopsis thaliana NHX Na+/H+antiporters in the salt stress response. Plant Journal 30, 529-39.

Zhang GH, Su Q, An LJ, Wu S. 2008. Characterization and expression of a vacuolar Na+/H+antiporter gene from the monocot halophyte Aeluropuslittoralis. Plant Physiology and Biochemistry46, 117-26.

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