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Quorum sensing-inhibition activities of philippine ethnobotanicals against virulence factors in Pseudomonas aeruginosa

Karmina N. Barrogo, Wilson R. Jacinto, Khristina G. Judan Cruz

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Int. J. Biosci.13(4), 173-182, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.173-182


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The global health issue of antibiotic resistance has brought about the discovery of a promising approach to control bacteria without developing resistant strains through quorum sensing inhibition (QSI) compounds. Among the most promising sources of novel QSI agents are the ethnobotanicals, plants that are utilized by ethnic communities as sources of medicine. Ten ethnobotanicals from the Igorot community of Imugan, Nueva Vizcaya, Philippines were screened to determine QSI activity against P. aeruginosa PNCM 1335.The methanolic extracts were tested for the presence of antibacterial activity using disk diffusion assay. In the absence of antibacterial property, the methanolic extracts were tested for QSI activities in reference to the expression of virulence factors swarming motility and pyocyanin production when the extracts were incorporated into the growth media.  None of the methanolic extracts showed antimicrobial activity against P.aeruginosa PNCM 1335. All extracts manifested effect on swarming motility on the contrary, no methanolic extracts were found to inhibit pyocyanin production in P. aeruginosa PNCM 1335.The ethnobotanicals show considerable potential as sources of QS inhibitory compounds for new therapeutic direction in preventing pathogenicity without the threat for development of bacterial resistance.


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Quorum sensing-inhibition activities of philippine ethnobotanicals against virulence factors in Pseudomonas aeruginosa

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