Rapid in vitro clonal propagation of a hybrid muskmelon (Cucumis melo L.) cultivar from seedling explants

Paper Details

Research Paper 01/01/2012
Views (228) Download (12)
current_issue_feature_image
publication_file

Rapid in vitro clonal propagation of a hybrid muskmelon (Cucumis melo L.) cultivar from seedling explants

Hafizur Rahman, M Shahinozzaman, M Rezaul Karim, M Aminul Hoque, M Monzur Hossain, AKM Rafiul Islam
Int. J. Agron. Agri. Res.2( 1), 47-52, January 2012.
Certificate: IJAAR 2012 [Generate Certificate]

Abstract

An investigation was undertaken to develop a reliable strategy for large scale multiplication of a hybrid cultivar of muskmelon through in vitro clonal propagation. Best in vitro seed germination was observed in MS medium containing 0.5 mg/l GA3. Two explants excised from axenic seedlings were tested for shoot proliferation and leaf node showed better shoot proliferation than shoot tip explants. Maximum shoots (8.22±0.23) per culture were produced from leaf node explants cultured on MS medium augmented with 1.0 mg/l BA. Rooting was found best on 0.1 mg/l NAA containing medium. Rooted plantlets were acclimatized gradually and transferred to the field condition and finally, 82 % plantlets were survived well.

VIEWS 8

Ahmad N, Anis M. 2005. In vitro mass propagation of Cucumis sativus L. from nodal segments. Turkish Journal of Botany 29, 237-240.

Diaz DH, Martin GC. 1971. Peach seed dormancy in relation to inhibitors and applied growth substance. Journal of American Society for Horticultural Science 97(5), 651-654.

Hoque ME, Bhomik A, Khalequzzaman M. 1998. In vitro culture of pointed gourd. Thai Journal of Agricultural Science 31, 369-374.

Keng CL, Hoong LK. 2005. In vitro plantlets regeneration from nodal segments of muskmelon. Biotechnology 4(4), 354-357.

Kumar S, Singh M, Srivastava K, Banerjee MK. 2003. In vitro propagation of pointed gourd (Trichosanthes dioica Roxb.). Cucurbit Genetics Cooperative Report 26, 74-75.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15 (3), 473- 497.

Nerson H. 2007. Seed Production and Germinability of Cucurbit Crops. Seed Science and Biotechnology 1(1), 1-10.

Sarowar S, Oh HY, Hyung NI, Min BW, Harn CH, Yang SK, Ok SH, Shin JS. 2003. In vitro micropropagation of a Cucurbita interspecific hybrid cultivar – a root stock plant. Plant Cell, Tissue and Organ Culture 75, 179-182.

Shalaby TA, Omran SA, Baioumi YA. 2008. In  vitro  propagation  of  two  triploid  hybrids  of watermelon through adventitious shoot organogenesis and shoot tip culture. Acta Biologica Szegediensis 52(1), 27-31.

Sultana RS, Bari MA. 2003. Effect of different plant growth regulators on direct regeneration of watermelon (Citrulus lanatus Thumb.) Plant Tissue Culture 13(2), 173-177.

Tarsem  L,  Raman  S,  Vashisht  VK.  2005. Micro-Propagation of Muskmelon (Cucumis melo L.). Crop Improvement 32(2), 214-218.