Regulatory mechanisms in the interaction between plants and pathogens – a proteomics approach

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Review Paper 01/08/2014
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Regulatory mechanisms in the interaction between plants and pathogens – a proteomics approach

Atefe Kahe, Mahmoud Toorchi, a Sanaz Adalatzadeh-Aghdam
J. Bio. Env. Sci.5( 2), 192-200, August 2014.
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Plants respond to stresses with inductive responses. Induction responses include chemical and structural defense responses that only activate after pathogen attack. Cellular responses greatly coordinated and with identification of pathogens and transduction pathways cause to minimize contamination. Regulatory mechanisms in the interaction between plants and pathogens are complex and dynamic. Proteomics techniques due to identification of new proteins in relation with their role are useful for understanding these regulatory networks. Proteomics is a careful method to study proteins especially expression, structure and molecular role of them. The goal of this technique is identification and description of all proteins expresses in a biological system.


Abuqamar S, Luo SH, Laluk K, Mickelbart MVT and Mentiste T. 2009. Crosstalk between biotic and abiotic stress responses in tomato is mediated by the AIM1 transcription factor. Plant Journal 58, 347-360.

Afroz A, Ali GM, Mir A, Komatsu S. 2011. Application of proteomics to investigate stress-induced proteins for improvement in crop protection. Plant Cell Repeat 30, 745-763.

Agrawal GK, Jwa NS, Rakwal R. 2009. Rice proteomics: ending phase I and the beginning of phase II. Proteomics 9, 935-963.

Agrawal GK, Rakwal R. 2008. Plant proteomics: technologies strategies, and applications. John Wiley and Sons, Inc, Hoboken New jersy.

Akiyama T, Pillia MA. 2001. Molecular cloning characterization and in vitro expression of a novel endo-1, 3-glucanase up-regulated by ABA and drought stress in rice (Oryza sativa L.). Plant Science 161, 1089-1098.

Bantignies B, Seguin J, Muzac I, Dedaldechamp F, Gulick P, Ibrahim R. 2000. Direct evidence for ribonucleolytic activity of a PR-10 like protein from white lupin roots.Plant Molecular Biology 42, 871-881.

Beckers GJM, Jaskiewicz M, Liu Y, Underwood WR, He SY, Zhang S, Conra U. 2009. Mitogen-activated protein kinases 3 and 6 are required for full priming of stress responses in Arabidopsis thaliana. Plant Cell 21, 944-953.

Benschop JJ, Mohammed S, O’Flaherty M, Heck AJR, Slijper M, Menke FLH. 2007. Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis. Molecular and Cellular Proteomics 6, 1198-1214.

Bera S, Purkayastha RP. 1997. Identification and characterization of some PR-proteins induced by kitazin and Rhizoctoniasolani causing sheath blight of rice. Indian Journal of Experimental Biology 35, 644-649.

Bhatia Y, Mishra S, Bisaria VS. 2002. Microbial β-glucosidases: cloning, properties, and applications. Critical Reviews in Biotechnology 22, 375-407.

Boller T. 1988. Ethylene and the regulation of antifungal hydrolases in plant. Oxford Surveys of Plant Molecular and Cell Biology 5, 145-174.

Bowles DJ. 1990. Defence-related proteins in higher plants.Annual Review Biochemistry 59, 873-907.

De Freitas CDT, Sousa Nogueira FC, Vasconcelos IM, Oliveira JTA, Domont GB. Ramos MV. 2011. Osmotin purified from the latex of Calotropisprocera: biochemical characterization, biological activity and role in plant defense. Plant Physiology and Biochemistry 49, 738-743.

Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, YamaguchiShinozaki K, Shinozaki K. 2006. Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Current Opinion in Plant Biology 9, 436-442.

GarciaBrugger A, Lamotte O, Vandelle E, Bourque S, Lecourieux D, Poinssot B, Wendehenne D, Pugin A. 2006. Early signaling events induced by elicitors of plant defenses. Molecular Plant–Microbe Interaction 19, 711-724.

Glazebrook J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annuaal Reviews of Phytopathology 43, 205-227.

Hennig J, Dewey RE, Cutt JR, Klessig DF. 1993. Pathogen, salicylic acid and developmental dependent expression of a β-1, 3-glucanase/GUS gene fusion in transgenic tobacco plants.Plant Journal 4, 481-493.

Howe, G. A. and Schilmiller, A. L. 2002. Oxylipin metabolism in response to stress. Current Opinion in Plant Biology 5, 230-236.

Huang D, Wu W, Abrams SR, Cutler AJ. 2008. The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. Experimental Botany 59, 2991-3007.

Hwang DH, kim ST, Kim SG, Kang KY. 2007. Comprehensive analysis of the expression of twenty-seven beta 1, 3-glucanase genes in rice (Oryza sativa L.). Molecular Cell 23, 207-214.

Jones JDG, Dangl JL. 2006. The plant immune system. Nature 444, 323-329

Jorrin-Novo JV, Maldonado AM, EchevarriaZomeno S, Valledor L, Castillejo MA, Curto M, Valero J, Sghaier B, Donoso G, Redondo I. 2009. Plant proteomics update (2007-2008): second generation proteomic techniques, an appropriate experimental design, and data analysis to fulfil MIAPE standards, increase plant proteome coverage and expa[nd biological knowledge. Proteomics 72, 285-314.

Jwa NS, Agrawalb Gk, Tamogamic S, Yonekurad M, Hane O, Iwahashif H, Rakwalb R. 2006. Role of defense/stress-related marker genes proteins and secondary metabolites in defining rice self-defense mechanisms. Plant Physiology and Biochemistry 44, 261-273.

Kaku, H., Nishizawa, Y., IshiiMinami, N., AkimotoTomiyama, C., Dohmae, N., Takio, K., Minami, E. and Shibuya, N. 2006. Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor. Proceeding of the National Academy of Sciences of the United States of America 103, 11086-11091.

Kav NNV, Srivastava S, Yajima W, Sharma N. 2007. Application of proteomics to Investigate plant-microbe interaction. Current Proteomics 4, 28-43.

Kim ST, Cho KS, Yu S, Kim SG, Hong JC, Han CD, Bae DW, Nam MH, Kang KY. 2003. Proteomic analysis of differentially expressed protein induced by rice blast fungus and elicitor in suspension-cultured rice cells. Proteomics 3, 2368-2378 (a)

Kim JK, Jang IC, Wu R, Zuo WN, Boston RS, Lee YH, Ahn IP, Nahm BH. 2003. Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight. Transgenic Research 12, 475-484 (b)

Kim ST, Kang YH, Wang Y, Wu J, Park ZY, Rakwal R, Agrawal GK, Lee SY, Kang KY. 2009. Secretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor. Proteomics 9, 1302-1313.

Kim ST, Kim SG, Hwang DH, Kang SY, Kim HJ, Lee BH, Lee JJ, Kang KY. 2004. Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthegrisea. Proteomics 4, 3569-3578.

Konishi H, Ishiguro K, Komatsu K. 2001. A proteomics approach towards understanding blast fungus infection of rice grown under different levels of nitrogen fertilization. Proteomics 1, 1162-1171.

Lee J, Bricker TM, Lefevre M, Mpinson S, Oard J. 2006. Proteomic and genetic approaches to identifying defence-related proteins in rice challenged with the fungal pathogen RhizoctoniaSolani. Molecular Plant Pathology 7, 405-416.

Linthorst HJM, Melchers LS, Mayer A, Van Roekel JSC, Cornelissen BJC, Bol JF. 1990. Analysis of gene families encoding acidic and basic-1,3glucanases of tobacco. Proceedings of the National Academy of Sciences 87, 8756-8760.

MaldonadoCaldern MT, SeplvedaGarc E, RochaSosa M. 2012. Characterization of novel F-box proteins in plants induced by biotic and abiotic stress. Plant Science 185, 208-217.

Maruyama K, Takeda M, Kidokoro S, Yamada K, Sakuma Y, Urano K, Fujita M, Yoshiwara K, Matsukura S, Morishita Y, Sasaki R, Suzuki H, Saito K, Shibata D, Shinozaki K, YamaguchiShinozaki K. 2009. Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated DREB1A and DREB2A. Plant Physiology 150, 1972-1980.

Meyers BC, Kaushik S, Nandety RS. 2005. Evolving disease resistance genes. Current Opinion in Plant Biology 8, 129-134.

Mithfer A, Ebel J, Felle HH. 2005. Cation fluxes cause plasma membrane depolarization involved in glucan elicitor-signaling in soybean roots. Molecular Plant-Microbe Interaction 18, 983-990.

Nishizawa Y, Saruta M, Nakazono K, Nishion Z. 2003. Characterization of transgenic rice plsnt over-expressing th stress-inducible beta-glucanase gene Gns1. Plant Molecular Biology 51, 43-52.

Quirino BF, Candido ES, Campo PF, Franco OL, Kruger RH. 2010. Proteomic approaches to study plant-pathogen interaction. Phytochemistry 71, 351-362.

Rakwal R, Komatsu S. 2000. Role of jasmonate in the rice (Oryza sativa L.) self-defense mechanism using proteome analysis. Electrophoresis 21, 2492-2500.

Rossignol M, Peltier JB, Mock HP, Matros A, Maldonado AM, Jorrin JV. 2006. Plant proteome analysis: a 2004-2006 update. Proteomics 6,5529-5548.

Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, Hunt MD. 1996. Systemic acquired resistance. The Plant Cell 8, 1809-1819

Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J. 2002. Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2, 1131-1145.

Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, Somerville SC, Manners JM. 2000. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proceeding of the National Academy of Sciences of the United States of America 97, 11655-11660.

Singh NK, Bracker CA, Hasegawa PM, Handa AK, Buckel S, Hermodson MA, Pfankoch E, Regnier FE, Bressan RA. 1987. Characterization of osmotin a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiology 85, 529-536.

Srivastava S, Rahman MH, Shah S, Kav NNV. 2006. Constitutive expression of the pea ABA-responsive 17 (ABR17) cDNA confers multiple stress tolerance in Arabidopsis thaliana. Plant Biotechnology 4,529-549.

Sticher L, MauchMani B, Metraux JP. 1997. Systemic acquired resistance. Annual Review of Phytopathology 35, 235-270.

Thomashow MF. 2010. Molecular basis of plant cold acclimation: insights gained from studying the CBF cold response pathway. Plant Physiology 154, 571-577.

Tuteja N. 2011. Chaperones and foldases in endoplasmic reticulum stress signaling in plants. Plant Signaling and Behavior 6, 232-236.

Van Loon LC, Van Kammen A. 1970. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotianatabacum var. ‘‘Smsun’’ and ‘‘Samsun NN’’ II Changes in protein constitution after infection with tobacco mosaic virus. Virology 40,199-206.

Van Loon LC, Van Strien EA. 1999.The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology 55, 85-97.

Velazhahan R, ChenCole K, Anuratha CS, Muthukrishnan S. 1998. Induction of thaumatin-like protein (TLPs) in rhizoctoniasolania infected rice and characterization of two new cDNAclone. PhysiologiaPlantarum 102, 21-28.

Vitamvas P, Kosova K, Prasil IT. 2007. Proteome analysis in plant stress.Czech Journal of Genetics and Plant Breeding 43,1-6.

Wang Y, Kim SG, Kim ST, Agrawal GK, Rakwal R, Kang KY. 2011.Biotic stress-responsive rice proteome. Plant Biology 54, 219-226.

Weckwerth W. 2008. Integration of metabolomics and proteomics in molecular plant physiology-coping with the complexity by data-dimensionality reduction.PhysiologiaPlantarum 132, 176-189.

Wienkoopa S, Baginskyb S, Weckwertha W. 2010. Arabidopsis thaliana as a model organism for plant proteome research. Proteomics 73, 2239-2248.

Yamaguchi T, Nakayama K, Hayashi T, Tanaka Y, Koike S. 2002. Molecular cloning and characterization of a novel β1,3-glucanase gene from rice. Bioscience Biotechnology and Biochemistry 66, 1403-1406.

Yanischperron C, Vieira J, Messing J. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mp 18 and pUC 19 vectors. Gene 33, 103-119.

Zeng LR, VegaSanchez ME, Zhu T, Wang G L. 2006. Ubiquitination-mediated protein degradation and modification: an emerging theme in plant-microbe interactions. Cell Research 16, 413-426.

Zhang X, Fang A, Riley C, Wang M, Regnier F, Buck C. 2010. Multi-dimensional liquid chromatography in proteomics.AnalyticaChimicaActa 664, 101-113.