Resistance Characterization of Cultivated Varieties and Rice Wild Species in Response to Bacterial Blight

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Research Paper 01/12/2015
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Resistance Characterization of Cultivated Varieties and Rice Wild Species in Response to Bacterial Blight

Abdul Waheed, Habib Ahmad, Fida. M. Abbasi, Azhar H. Shah, Hamid Ali, F. S. Hamid, Saqib Mumtaz
Int. J. Biosci.7( 6), 103-110, December 2015.
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Abstract

Bacterial leaf blight (BB) of rice caused by (Xanthomonas oryzae pv oryzae) is converting into a critical threat almost in all rice growing countries of the world. In order to catagorize resistant sources to virulent isolates of BB, an experiment comprising 02 species of wild rice (Oryza sp.) and four most common cultivated varieties i.e., Bas-385 , Swat-1 , JP-5 and Fakhar Malakand of rice in Pakistan was conducted in the green house of Genetic Department Garden campus ,Hazara University in the rice growing season during 2012. Bacterial suspension of concentration 108 CFU/ml was prepared from mixture of (Xanthomonas oryzae pv oryzae) prevailing in Khyber Puktunkhawa,Pakistan i.e., X00-1, X00-2 and X00-3. Clip method of artifical inoculation was used. Both tested wild relatives of rice O. longistaminata and O. rufipogon showed highly resistance to all the isolate. F3 genotypes Bas-385 x O. rufipogon was found highly susceptible to most of the isolates among all others genotypes. The use of resistant wild species O. rufipogon is therefore recommended in rice breeding program for transfer of bacterial blight resistant genes to cultivated varieties to enhance the relative characters.

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