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Resistance of Kenyan wheat germplasm to Fusarium head blight and deoxynivalenol contamination

Oliver O. Okumu, James W. Muthomi, Charity K. Mutegi, John M. Wagacha

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Int. J. Agron. Agri. Res.9(2), 22-35, August 2016


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Fusarium head blight (FHB) of wheat causes quantitative and qualitative reduction in yield. Cultivar resistance is the most effective method of managing the disease. This study evaluated the resistance of wheat germplasm currently available in Kenya to Fusarium head blight and deoxynivalenol (DON) contamination. Nine wheat varieties and four CIMMYT lines were evaluated for susceptibility to FHB under two diverse agro-ecologies in Nakuru and Narok Counties, Kenya during the 2013 cropping season and in the greenhouse. The varieties and lines were inoculated at mid-anthesis with mixed inocula of three isolates of F. graminearum. Incidence and severity of FHB were assessed weekly and data on severity used to calculate the area under disease progress curve (AUDPC). After harvest, incidence of F. graminearum in the grain was determined and DON contamination determined by direct competitive enzyme-linked immunosorbent assay (ELISA). Incidence and severity of FHB differed significantly (p ≤ 0.05) among the varieties and lines with variety Kwale showing the least disease while line 10155 had the highest FHB levels. The AUDPC ranged from 69.8 to 120.1 for the least and most susceptible varieties, respectively. All the wheat lines and varieties accumulated DON ranging from 442 to 748 ng/g (Mean = 572 ng/g). There was a positive correlation between FHB severity, AUDPC, re-isolation frequency of F. graminearum and DON accumulation. The assessed wheat varieties and lines could be grouped into two categories: moderately tolerant and susceptible. Wheat varieties and lines available in Kenya are susceptible to FHB and DON contamination implying need for considering other strategies for managing FHB.


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Resistance of Kenyan wheat germplasm to Fusarium head blight and deoxynivalenol contamination

Abate Z, Liu S, Mckendry AL. 2008. Quantitative trait loci associated with deoxynivalenol content and kernel quality in the soft red winter wheat Ernie. Crop Science 48, 1408-1418. http://dx.doi.org/10.2135/cropsci2007.07.0411

Afsah-Hejri L, Jinap S, Hajeb P, Radu S, Shakibazadeh S. 2013. A review on mycotoxin in food and feed: Malaysia case study. Comprehensive Reviews in Food Science and Food safety 12, 629-651. http://dx.doi.org/10.1111/1541-4337.12029

Arseniuk E, Foremska E, Goral T, Chelkowski J. 1999. Fusarium head blight reactions and accumulation of deoxynivalenol (DON) and some of its derivatives in kernels of wheat, triticale, and rye. Journal of Phytopathology 147, 577-590. http://dx.doi.org/10.1046/j.1439-0434.1999.00433.x

Bai GH, Shaner G. 1994. Scab of wheat – prospects for control. Plant Disease 78, 760-766. http://dx.doi.org/10.1094/PD-78-0760

Bai GH, Plattner R, Desjardins A, Kolb F. 2001. Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding 120, 1-6. http://dx.doi.org/10.1046/j.1439-0523.2001.00562.x

Buerstmayr H, Legzdina L, Steiner B, Lemmens M. 2004. Variation for resistance to Fusarium head blight in spring barley. Euphytica 137, 279-290. http://dx.doi.org/10.1023/B:EUPH.0000040440.99352.b9

Chrpova J, Sip V, Matejova E, Sykorova S. 2010. Resistance of winter wheat varieties registered in the Czech Republic to mycotoxin accumulation in grain following inoculation with Fusarium culmorum. Czech Journal of Genetics and Plant Breeding 43, 44-52.

Chrpova J, Sip V, Stockova L, Stehno Z, Capouchora I. 2013. Evaluation of resistance genes to FHB in spring wheat genotypes belonging to various Triticum spp. Czech Journal of Genetics and Plant Breeding 46, 122-134.

Cirlini M,  Generotti S, Dall’erta A, Lancioni P, Ferrazzano G, Massi A,  Galaverna G, Dall’asta A. 2014. Durum wheat (Triticum Durum Desf.) lines show different abilities to form masked mycotoxins under greenhouse conditions. Toxins 6, 81-89. http://dx.doi.org/10.3390/toxins6010081

Cowger C, Patton-Ozkurts J, Brown-Guedira G, Perugini L. 2009. Post-anthesis moisture increased Fusarium head blight and deoxynivalenol levels in North Carolina winter wheat. Phytopathology 99, 320–327. http://dx.doi.org/10.1094/PHYTO-99-4-0320

D’mello JPF, Placinta CM, Macdonald AMC. 1999. Fusarium mycotoxins: A review of global implications for animal health, welfare and productivity. Animal Feed Science and Technology 80, 183-205. http://dx.doi.org/10.1016/S0377-8401(99)00059-0

Desjardins AE. 2006. Fusarium Mycotoxins: Chemistry, Genetics, and Biology. St Paul, MN: American Phytopathological Society.

Geddes J, Eudes F, Tucker JR, Legge WG, Selinger LB. 2008. Evaluation of inoculation methods on infection and deoxynivalenol production by F. graminearum on barley. Canadian Journal of Plant Pathology 30, 66-73. http://dx.doi.org/10.1080/07060660809507497

Gilbert J, Tekauz A. 2011. Strategies for management of Fusarium head blight in cereals. Prairie Soils and Crops Journal 4, 97-104.

Gomez AK, Gomez AA. 1983. Statistical procedures for agricultural research, 2nd edition. New York City, NY: John Wiley and Sons, 680.

Grausgruber H, Lemmens M, Burstmayr H, Ruckenbauer P. 1995. Evaluation of inoculation methods for testing Fusarium head blight resistance of winter wheat on single plant basis. Bodenkultur 46, 39-49.

Hernandes-Nopsa JF. 2010. Fusarium head blight: Winter wheat cultivar responses and characterization of pathogen isolates. PhD thesis, University of Nebraska-Lincoln, USA.

Jia HY, Cho SH, Muehlbauer GJ. 2009. Transcriptome analysis of a wheat nearisogenic line pair carrying Fusarium head blight-resistant and susceptible alleles. Molecular Plant Microbiology 22, 1366-1378. http://dx.doi.org/10.1094/MPMI-22-11-1366

Kazan K, Gardiner MD, Manners MJ. 2012. On the trail of a serial killer: recent advances in Fusarium graminearum pathogenic and host resistance. Molecular Plant Pathology 13, 399-413. http://dx.doi.org/10.1111/j.1364-3703.2011.00762.x

Kubo K, Kawada N, Fujita N. 2013. Evaluation of FHB in wheat and the development of a new variety by integrating type I and type II resistance. Japan Agricultural Research Quarterly 47, 9-19. http://dx.doi.org/10.6090/jarq.47.9

Lacey J, Bateman GL, Mirocha CJ. 1999. Effects of infection time and moisture on development of ear blight and deoxynivalenol production by Fusarium spp. in wheat. Applied Biology 134, 277–283. http://dx.doi.org/10.1111/j.17447348.1999.tb05265.x

Landschoot S, Waegeman W, Audenaert K, Vandepitte J, Baetens JM, De Baets B, Haesaert G. 2012. An empirical analysis of explanatory variables affecting Fusarium head blight infection and deoxynivalenol content in wheat. Journal of Plant Pathology 94, 135–147. http://dx.doi.org/10.4454/jpp.fa.2012.021

Leslie JF, Summerell BA. 2006. The Fusarium laboratory manual. 2006. Ames, Iowa: Blackwell Publishing Professional.

Lindblad M, Borjesson T, Hietaniemi V, Elen O. 2012. Statistical analysis of agronomical factors and weather conditions influencing deoxynivalenol  levels in oats in Scandinavia. Food Additives and Contaminants 29, 1566–1571. http://dx.doi.org/10.1080/19440049.2011.647335

Liu S, Griffey CA, Marla DH, Anne LM, Chen J, Brooks WS, Brown‑Guedira G, Sanford D, Schmale DG. 2013. Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars. Theory of Applied Genetics 126, 2485-2498. http://dx.doi.org/10.1007/s00122-013-2149-y

Mcmullen MS, Halley B, Schatz S, Meyer J,  Jordahl J, Ransom J. 2008. Integrated strategies for Fusarium head blight management in the United States. Cereal Research Communications 36, 563-568. http://dx.doi.org/10.1556/CRC.36.2008.Suppl.B.45

Merhej J, Richard-Forget F, Barreau C. 2011. Regulation of trichothecene biosynthesis in Fusarium: Recent advances and new insights. Applied Microbiology and Biotechnology 91, 519-528. http://dx.doi.org/10.1007/s00253-011-3397-x

Mesterhazy A. 1995. Types and components of resistance to Fusarium head blight on wheat. Plant Breeding 144, 377–386. http://dx.doi.org/10.1111/j.14390523.1995.tb00816.x

Mesterhazy A. 1997. Methodology of resistance testing and breeding against Fusarium head blight in wheat and results of selection. Cereal Research Communications 25, 631-637.

Mesterhazy A, Bartok T, Lamper C. 2003. Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the efficacy of fungicides for control of Fusarium head blight. Plant Disease 87, 1107-1115. http://dx.doi.org/10.1094/PDIS.2003.87.9.1107

Mesterhazy A, Bartok T, Kaszonyi G, Varga M, Toth B, Varga J. 2005. Common resistance to different Fusarium species causing Fusarium head blight in wheat. European Journal of Plant Pathology 112, 267-281. http://dx.doi.org/10.1007/s10658-005-2853-9

Mesterhazy A, Toth B,  Varga M, Bartok T, Szabo-Hever T,  Farady L, Lehoczki-Krsjak S. 2011. Role of fungicides, application of nozzle types, and the resistance level of wheat varieties in the control of Fusarium head blight and deoxynivalenol. Toxins 3, 1453-1483. http://dx.doi.org/10.3390/toxins3111453

Miedaner T, Gang G Geiger HH. 1996. Quantitative-genetic basis of aggressiveness of 42 isolates of Fusarium culmorum for winter rye head blight. Plant Disease 80, 500-504. http://dx.doi.org/10.1094/PD-80-0500

Musyimi SL, Muthomi JW, Narla RD, Wagacha JM. 2012. Efficacy of biological control and cultivar resistance on Fusarium head blight and T-2 toxin contamination in wheat. American Journal of Plant Sciences 3, 599-607. http://dx.doi.org/10.4236/ajps.2012.35073

Muthomi JW. 2001. Comparative studies on virulence, genetic variability and mycotoxin production among isolates of Fusarium species infecting wheat. PhD thesis, University of Nairobi, Kenya.

Muthomi JW, Ndungu JK, Gathumbi JK, Mutitu EW, Wagacha JM. 2008. The occurrence of Fusarium species and mycotoxins in Kenyan wheat. Crop Protection 27, 1215-1219. http://dx.doi.org/10.1016/j.cropro.2008.03.001

Muthomi JW, Ndung’u JК, Chemining’wa GN, Wagacha JM. 2007. Reaction of some Kenyan wheat cultivars to head blight after inoculation with Fusarium graminearum. Asian Journal of Plant Sciences 6, 585-591. http://dx.doi.org/10.3923/ajps.2007.585.591

Muthomi JW, Oerke EC, Dehne, HW, Mutitu EW. 2002. Susceptibility of Kenyan wheat varieties to head blight, fungal invasion and deoxynivalenol accumulation inoculated with Fusarium graminearum. Journal of Phytopatholology 150, 30-36. http://dx.doi.org/10.1046/j.1439-0434.2002.00713.x

Nelson PE, Toussoun TA, Marasas WFO. 1983. Fusarium species: An illustrated manual for identification. University Park, Pa: Pennsylvania State University Press.

Nirenberg HI. 1981. A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany 59, 1599-1609. http://dx.doi.org/10.1139/b81-217

Oliver RE, Cai X, Xu SS, Chen X, Stack RW. 2005. Wheat alien species derivatives: A novel source of resistance to Fusarium head blight in wheat. Crop Science 45, 1353-1360. http://dx.doi.org/10.2135/cropsci2004.0503

Ortega LM, Kikot GE, Andrea LA Teressa MA. 2013. Screening of Fusarium graminearum isolates for enzymes extracellular and DON production. Journal of Mycology 2013, 1-7. http://dx.doi.org/10.1155/2013/358140

Parry DW, Jenkinson P, Mcleod L. 1995. Fusarium ear blight (scab) in small grain cereals-a review. Plant Pathology 44, 125-126. http://dx.doi.org/10.1111/j.1365-3059.1995.tb02773.x

Paul PA, Lipps PE, Madden LV. 2005. Relationship between visual estimates of Fusarium head blight intensity and deoxynivalenol accumulation in harvested wheat grain: A meta-analysis. Phytopathology 95, 1225–1236. http://dx.doi.org/10.1094/PHYTO-95-1225

Payne RW, Harding SA, Muray DA, Soutar DM, Baird DB, Welham SJ, Kane AF, Gilmour AR, Thompson R, Webster R, Tunnicliffe GW. 2008. Genstat® Release 11 Reference Manual. Hemel Hemstead, UK: VSN International Ltd.

Pestka JJ, Zhou HR, Moon Y. 2004. Cellular and molecular mechanisms for immune modulation by deoxynivalenol and other trichothecenes: Unraveling a paradox. Toxicology Letters 153, 61-73. http://dx.doi.org/10.1016/j.toxlet.2004.04.023

Ransom JK, Mcmullen MP. 2008. Yield and disease control on hard winter wheat cultivars with foliar fungicides. Agronomy Journal 100, 1130-1137. http://dx.doi.org/10.2134/agronj2007.0397

Schroeder HW, Christensen JJ. 1963. Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53, 831-838.

Shanner G, Finney RE. 1977. The effect of nitrogen fertilization on the expression of slow mildewing resistance in knox wheat. Phytopathology 67, 1051-1056. http://dx.doi.org/10.1094/Phyto-67-1051

Sip V, Chrpova J, Sykorova S. 2008. Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolates. Czech Journal of Genetics and Plant Breeding 44, 43-59.

Stepien L, Chelkowski J. 2010. Fusarium head blight of wheat: Pathogenic species and their mycotoxins. World Mycotoxin Journal 3, 107-119. http://dx.doi.org/10.3920/WMJ2009.1193

Usele G, Beinarovica I, Mezaka I, Legzdina L. 2013. Comparison of spring barley (Hordeum vulgare) screening methods for FHB resistance breeding. Zemdrbyste-Agriculture 100, 317-324. http://dx.doi.org/10.13080/z-a.2013.100.041

Wegulo SN. 2012. Factors influencing deoxynivalenol accumulation in small grain cereals. Toxins 4, 1157–1180. http://dx.doi.org/10.3390/toxins4111157

Wegulo SN, Bockus WW, Hernandez Nopsa J, De Wolf ED, Eskridge KM, Peiris KHS, Dowell FE. 2011. Effects of integrating cultivar resistance and fungicide application on Fusarium head blight and deoxynivalenol in winter wheat. Plant Disease 95, 554-560. http://dx.doi.org/10.1094/PDIS-07-10-0495

Zadocks JC, Chang TT, Konzak CF. 1974. Decimal code for growth stages of cereals. Weed Research 15, 415- 421. http://dx.doi.org/10.1111/j.1365-3180.1974.tb01084.x

Zhang L, Luo P, Ren Z, Zhang H. 2011. Controlling Fusarium head blight of wheat (Triticum aestivum L.) with genetics. Advances in Bioscience and Biotechnology 2, 263-270. http://dx.doi.org/10.4236/abb.2011.24038

Zhu H, Gilchrist L, Hayes P, Kleinhofs A, Kudrna D, Liu Z, Viva H. 1999. Does function follow form? Principal QTLs for Fusarium head blight (FHB) resistance are coincident with QTLs for inflorescence traits and plant height in a doubled-haploid population of barley. Theoretical and Applied Genetics 99, 1221-1232. http://dx.doi.org/10.1007/s001220051328


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