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Response in chickpea (Cicer arietinum L.) seedling growth to seed priming with iron oxide nanoparticles

Research Paper | March 1, 2019

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V.A. Pawar, J.D. Ambekar, B.B. Kale, S.K. Apte, S.L. Laware

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Int. J. Biosci.14( 3), 82-91, March 2019

DOI: http://dx.doi.org/10.12692/ijb/14.3.82-91


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The increasing application of nanoparticles (NPs) in various fields has made it crucial to study its impact on environment. Considering the positive and negative effect, study of nanoparticles is of concern in crops. It is known to directly influence crop growth and also enter food chain affecting living beings. Therefore, the present study was undertaken with the aim to evaluate the effect of iron oxide nanoparticles on seedling growth in Chickpea (Cicer arietinum L.) variety Digvijay. Seed priming was carried out using different concentrations of iron oxide (Fe2O3) NPs with an increment of 4 µg/ml ranging from 4 to16 µg/ml. Starch (10%) was used as a coating agent. The primed seeds were air dried and further used to study growth of seedling by paper towel method for germination in vitro and in vivo study as well. The grown seedlings were evaluated for growth parameters. Significant observations were noticed for seed germination and growth parameters viz. shoot length, root length, root to shoot ratio, fresh and dry weight that were considered as crucial indicators. These indicators summarises cumulative growth enhanced in seeds primed with lower concentrations up to 12 µg/ml Fe2O3 NPs and inhibits further growth at higher concentrations. Hence, from the results it demonstrates that optimised dose levels of Fe2O3 NPs can be used as co-fertilizer to improve growth in chickpea at lower concentrations.


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Response in chickpea (Cicer arietinum L.) seedling growth to seed priming with iron oxide nanoparticles

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